The search for novel components with antibacterial potential has recently gained increasing significance due to growing worldwide bacterial infections by antibiotic-resistant bacterial strains. Therapeutic plants are crucial to human health due to their antiseptic potential against bacterial pathogens. Botanical sources have benefitted healthcare for many years due to the various active compounds present, such as tannins, terpenoids, alkaloids, and flavonoids. This study seeks to report the antibacterial potential of Cuminum cyminum against Escherichia coli, Bacillus subtilis, Bacillus cereus, and Staphylococcus aureus. The research proceeded with the use of methanolic extract of C. cyminum preparation using the Soxhlet extraction technique. Formulating five concentrations (150, 180, 200, 220, and 250 mg/ml) used deionized water and incurred testing at varying temperatures (40 °C, 60 °C, 80 °C, 100 °C, and 121 °C) and pH (3, 5, 7, 9, and 11 pH) ranges. The well-diffusion assay and minimum inhibitory concentration helped analyze the antimicrobial properties of C. cyminum methanolic extract as observed against selected bacterial strains. The results’ analysis engaged the one-way ANOVA. It was evident that the maximum effect of plant extract against E. coli, B. subtilis, and B. cereus was at 200 mg/ml and against Staph. aureus, a maximum zone emerged at 180 mg/ml. At varying temperatures, the maximum inhibition of E. coli, Staph. aureus, and B. cereus occurred at 121 °C, with B. subtilis inhibited at 80 °C. For pH changes, it revealed that all strains were sensitive to acidic pH (3) at both concentrations (150 and 250 mg/ml).

Keywords: Cuminum cyminum, Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Bacillus cereus

Key findings: This study concluded that methanolic extracts of C. cyminum showed growth inhibition in all test strains. This inhibition might be due to cell membrane damages (caused by C. cyminum extract) leading to the restricted growth of the bacterial strain. Further studies need pursuits to validate this damage. This study also reported that C. cyminum methanolic extract proved effective against B. cereus compared with the other strains. The plant extract’s characterization also appeared at various ranges of temperature and pH.

Cysteine and thiourea represent natural antioxidants (compounds containing sulfur) that can protect plants against a broad spectrum of environmental stresses. A field trial directly assessed the probable influence of foliar treatment of cysteine (50, 75, and 100 mg L^-1) and thiourea (200, 400, and 600 mg L^-1) on growth parameters, photosynthetic pigments, seed yield quantity and quality, and some biochemical characteristics of flax plants grown in sandy soil. A foliar spray of cysteine and thiourea markedly increased growth characters, concurrently with an increase in Indole acetic acid content and photosynthetic pigments. Compatible osmolytes and yield components also improved compared with untreated plants. All treatments increased seed yield, oil content, and its components. The ratio of unsaturated fatty acids to saturated fatty acids bore enrichment in the yielded seeds. It was also noticeable that the 400 mg L^-1 thiourea was the most pronounced in increasing to maximum the tested parameters of the flax plant. It could be a conclusion that foliar sprays of cysteine and thiourea were active in ameliorating flax performance through increasing antioxidant compounds and enzymes.

Keywords: Flax, cysteine, thiourea, growth, osmoprotectant, antioxidants, yield, fatty acids

Key findings: The flax seed variety (Sakha-3) exhibited its maximum growth and yield by foliar spraying with 400 mg L^-1 thiourea or 75 mg L^-1 cysteine when cultivated in sandy soils, and this effect came as a response to improving the antioxidant defense system.

The experiment commenced in the crop season of 2021–2022 at two experimental locations—first at the Department of Field Crops, College of Agriculture and Forestry, University of Mosul, Iraq, and the second was at a farmer’s field in Dohuk Governorate, Iraq. The presented study assessed the two wheat cultivars (Rasheed and Buhouth1) and their response to different concentrations of bread yeast (0, 4, and 8 g L^-1), as the wheat plants incurred spraying with yeast powder. The experimental layout used the randomized complete block design (RCBD) with three replications. According to the results, cultivar Rasheed showed significant superiority for plant height (49.4 and 50.2 cm), spike length (6.9 and 7.3 cm), and spikes plant^-1 (5.7 and 6.1 spikes plant^-1), and Buhouth1 cultivar was substantially superior for grain yield (18.6 and 20.6 g plant^-1) and harvest index (34.77% and 40.26%) for both locations. Also, from the results, the time of adding bread yeast in the booting stage was superior for characteristics: spikes plant^-1 (5.9 and 5.7), grain yield (19.1 and 20.9 g plant^-1), and harvest index (34.85% and 40.42%), whereas the time of addition in the tillering stage was superior for the 1000-grain weight (36.97 and 36.66 g) and biological yield (58.4 and 56.0 g) for both locations. The bread yeast concentration findings revealed a considerable advantage of the concentrations at 4 and 8 g L^-1 (they did not differ significantly from one other) over the absence of bread yeast concentration of 0 g L^-1 in the tillers plant^-1, spike length, spikes plant^-1, and biological yield. In the harvest index %, the concentration above 4 g L^-1 was best for the two locations. Most binary and triple interactions of the components in this investigation revealed substantial changes, particularly when their part involved spraying with bread yeast for the two experimental locations.

Keywords: Wheat (Triticum aestivum L.), yield-related traits, environmental locations, Saccharomyces cerevisiae, yeast

Key findings: Cultivar Rasheed showed significant superiority for plant height, spike length, and spikes plant^-1, while Buhouth1 cultivar superiority was in grain yield and harvest index for both locations. On adding time of bread yeast, the time of addition in the booting stage was superior in the characteristics of spikes plant^-1, grain yield, and harvest index, whereas the time of addition in the tillering stage was superior in the characteristics of 1000-grain weight, biological yield, and the conc. of 4 and 8 g L^-1 compared with 0 g L^-1.

Tomatoes are low in calories, however, they provide the precious content of vitamins, minerals, organic acids, carbohydrates, and, especially, carotenoids (β-carotene and lycopene), as essential requirements for the normal functioning of the human body. The latest study aimed to quantify the various compounds that exhibit antioxidant activities in different tomato cultivars. Tomato landraces with the supreme content of individual chemicals and complex of varied traits can serve as genetic sources in breeding for obtaining the tomato genotypes with the highest content of carotenoids, ascorbic acid, phenols, dry matter, and soluble sugars. Comparative analysis of tomato cultivars showed that to obtain genetic material with an enhanced content of secondary metabolites, the following tomato cultivars are recommendable for the desired hybridization to make possible cross combinations, i.e., Black Jack, Shirley, Bosare blue, and Christmas Blueberry and can suggest more precisely for high carotenoids content. Tomato cultivars VS169-19 and Paul Robeson can benefit as sources of soluble sugars and antioxidants, such as ascorbic acid and polyphenols.

Keywords: Tomato (Solanum lycopersicum L.), vitamins, minerals, carbohydrates, carotenoids, antioxidants, phenolic compounds, ascorbic acid, soluble sugars

Key findings: The presented study determined the accumulation of secondary metabolites in the tomato fruits, with promising genotypes identified and selected for further probe through a hybridization program. A significant (P ≤ 0.05) positive correlation between the β-carotene and lycopene content and between ascorbic acid and total phenolic content attained establishment.

Egypt suffers from limited water resources, which threatens food security and reduces the chances of horizontal expansion by reclaiming new desert lands. Therefore, it was necessary to achieve this target for the productivity and quality of crops under harsh conditions. Field experiments proceeded at the Nubaria research station under sandy soil conditions to evaluate the effects of different silicon sources (silica gel, algae rich in silicon, and potassium silicate) on sugar beet yield and quality under different irrigation regimes (100%, 75%, and 50%) of water requirement (WR) during the growing seasons of 2020 and 2021. Results showed that potassium silicate was most effective for increasing chlorophyll content and growth parameters compared with other sources under water stress conditions. Also, improved nutrient contents in the root and shoot of sugar beet gave the highest values on N (0.58%, 2.54%), P (0.132%, 0.318%), K (0.42%, 1.05%), Fe (67.18, 83.28 ppm), and Zn (11.29, 12.73 ppm) content, respectively, when applied with K₂SiO₃ compared with deficit irrigation conditions. Stimulating rich-Si remains the most effective for enhancing the growth, quality, and yield of sugar beet grown under deficit irrigation regimes, which makes plants more resistant to weather conditions and water stress.

Keywords: Silicon sources, sugar beet, yield, sugar quality, water stress

Key findings: The application of stimulate rich-Si has been found to alleviate the harmful effects of water stress on sugar beet crops. Among the different sources of silicon, the application of stimulates rich-Si K₂SiO₃ and algae containing Si is the most effective in enhancing sugar beet yield by reducing water stress effects and improving growth, quality, and sugar yield under deficit irrigation conditions.

Potato (Solanum tuberosum L.) is a promising source of antioxidants with health benefits. The presented study evaluated 15 white and colored potato cultivars, comparing each for total antioxidant compounds. In this research, the skin samples showed higher levels of total phenolic, flavonoid, and anthocyanin compounds than tuber flesh samples. However, in tuber flesh samples, the total phenolic content ranged from 23.55 to 48.39, and the total flavonoid content varied from 9.54 to 21.24 mg 100 g^-1 of fresh weight. The highest total phenolic content occurred in the flesh of the purple potato cultivar Monakh (48.39 mg 100 g^-1 of fresh weight), and the highest total flavonoid content surfaced from the Udacha cultivar (21.24 mg 100 g^-1 of fresh weight). According to these results, potato cultivars with yellow and white tubers have demonstrated total phenolic and flavonoid contents comparable with colored tuber cultivars. However, tubers’ total phenolic and flavonoid contents showed no correlations with the total anthocyanin content (r = 0.3872 and r = 0.1947, respectively). The established results could be beneficial in developing potato cultivars with high concentrations of substances with high antioxidant activities, such as anthocyanins. Potato cultivars with the highest concentration of phenolics and anthocyanin can be alternative functional foods for human nutrition.

Keywords: Potato (Solanum tuberosum L.), white and colored potato cultivars, pigmented potato, phenolics, flavonoids, anthocyanin compounds

Key findings: The total phenolic content (TPC) and the total flavonoid content (TFP) showed moderate correlations with the color of potato tubers. Potato cultivars with white and yellow skin had relatively high phenolic and flavonoid content levels in the skin and flesh. Potato genotypes with high levels of phenolic can benefit breeding programs for developing new cultivars. Such varieties represent an important natural source of antioxidants with potentially beneficial effects on human health.

The presented manuscript deals with the issues of genesis and evolution of soil structure and crop plants in the Lower Amu Darya Region. The pertinent investigation also elaborates on wild plant characteristics in the desert zone and details of cultivated plants grown in the Lower Amu Darya region. The paper also discusses soil-forming processes and the structure of soils, which is the external appearance involved in specific changes from the vertical direction of its layers. Horizons always differ in color, structure, composition, and other morphological features. The article also describes the mechanical composition of the soils of these regions, including the formation of plants grown on highly saline soils, the morphology and characteristics of these plants, and the effective use of arable land to ensure food security in the country. Likewise, the most urgent problems today are increasing soil productivity and its protection and raising economic efficiency to obtain high-quality and ecologically clean products from every agricultural crop. The soils distributed in the lower Amudarya regions, including Karakalpakstan and Khorezm regions, are more or less saline. For the soils’ future effective use, salt-resistant and phytomeliorative crops, especially alfalfa, white corn, beets, melons, watermelons, and pumpkins, and oil crops, such as sesame, will give a high and high-quality harvest. As a result of the sand and dust-salty mixture rising from the dry bottom of the Aral Sea under the influence of the wind in the spring months, crops are drying up. Therefore, to prevent this, it is most practical and scientifically imperative to carry out phytomeliorative work in these areas to establish saxovull farms.

Keywords: Strategy, plant, land, soil mechanical elements, structure, natural meliorator, endemic, food, agriculture, Aral Sea Region, environmental protection

Key findings: The article discusses the issues of the genesis and evolution of plant and soil cover in the lower reaches of the Amu Darya River and the zoning of agricultural crops to develop ecological conditions and reclamation soils.

Developing lowland tomatoes (Solanum lycopersicum L.) is a better strategy to resist global warming. Tomato expansion also requires an efficient and organized selection process, including breeding the advanced segregating populations. Using selection indices in combination with biometrical analysis, such as principal component analysis, path analysis, and the Smith-Hazel index, can further improve the effectiveness of the selection concept. Therefore, the presented research aimed to evaluate the lowland F5 tomato lines through various selection indices and select the potential genotypes for further preliminary yield tests. The latest study proceeded in an augmented design, divided into four blocks, with a randomized complete block design as an environmental scheme. For comparison, the five standard tomato cultivars, consisting of Chung, Gustavi, Mawar, Tymoty, and Karina, underwent repeated planting in each block to compare with 45 F5 lines. All the tomato genotypes’ analysis used correlation, path, and principal component analyses, and the Smith-Hazel index. Based on the results, the selection indices with a multivariate approach and genetic analysis proved effective in selecting tomato lines in the F5 populations. The PCA, Smith-Hazel, and path analyses were the best approaches for creating selection indices formed by three characteristics, namely, yield, the number of fruits per bunch, and the number of branches. The selection indices recognized and recommended the 23 tomato F5 strains for further studies in the preliminary yield testing.

Keywords: Path analysis, PCA, selection criteria, Smith-Hazel index, Solanum lycopersicum

Key findings: The Smith-Hazel, PCA, and path analysis proved better approaches in forming the selection indices in the presented tomato research. Based on these indices, the selection index formed was 0.22, the yield + 0.1 numbers of fruits per bunch + 0.06 number of branches. The selection indices recognized 23 lowland tomato lines, recommended for inclusion in the preliminary yield tests. However, the five promising tomato strains were MC 74.12.8, KM30.5.2, MC 74.12.5, MC 29.4.6, and MC 29.4.5.

Rodent tuber (Typhonium flagelliforme Lodd.) has anticancer bioactive compounds, including beta-sitosterol, stigmasterol, octadecanoic, and hexadecenoic acids. Its mutant plants showed a higher cytotoxic effect on breast cancer cells than its wild-type plants. In rodent tuber mutants, the genebased molecular markers associated with anticancer compounds have not reached identification. Designing the primer pairs at SNP sites observed in rodent tuber mutant lectin genes led to the latest study developing SNAP molecular markers. The exon part region of previous lectin gene sequences at 500 bp long also underwent analysis. In such a sequence, a three-point mutation helped analyze the amino acid transformation. A successful design of a pair of primers based on non-synonymous SNP sites was specifically for SNP sites that cause variations in amino acids. A non-synonymous SNP at the base of 183 bp changes threonine to arginine. Lec183 distinguished rodent tuber mutant plants better from their wild type in the amplification. The Lec183 marker detected a lectin gene SNP’s G allele and analyzed rodent tuber plants, which produced an amplification that determined the G allele level. The Lec183 is an effective marker for selecting rodent tuber-lectin mutation sites in large populations. This way helped obtain the rodent tubers with the highest anticancer bioactive compound more precisely and rapidly.

Keywords: Rodent tuber (Typhonium flagelliforme Lodd.), lectin gene, anticancer bioactive compound, specific primer design, SNAP, allele-specific marker

Key findings: Specific molecular markers designed with gene-based markers associated with anticancer bioactive compounds in rodent tuber (Typhonium flagelliforme Lodd.) plants are yet for study. Through primer lec183, the specific alleles in lectin genes can help differentiate the mutant and wild-type plants. The SNAP marker based on the lectin gene sequence could probably improve the accuracy of the selection of lectin anticancer compounds in rodent tuber mutant plants.

Amylose content (AC) and percentage of chalkiness (PC) are the two most important quality parameters of rice (Oryza sativa L.). Breeding for high-quality medium-grain rice has been a decisive demand for the current market and consumption. This research aimed to study the crossing populations and the selection of early generations of promising combinations for evaluation in the next generations. The crossing populations’ assessment ensued for genetic variability (PCV and GCV), heritability (h2BS), and genetic advance (GAM) for the related traits. The promising rice population’s election for pure lines employed marker-assisted selection (MAS). The result showed three promising maternal cultivars (PY2, Sieu Ham Chau, and ML202) and paternal cultivars (M-202, Saturn, and Palmyra). Through the analysis of genetic parameters in the rice F₂ populations, the cross combination of ML202/M-202 was the choice because of its valuable genetic parameters expected with PCV and GCV ≥ 20%, h2BS ≥ 60%, and GAM ≥ 20% for both traits of AC and PC. The Chi-square test of the variables AC and PC in the cross-population ML202/M-202 indicated the segregated ratios of 3:1 in the phenotype and 1:2:1 in the genotype. Applying MAS to select the promising rice F₂ populations resulted in 92 individuals carrying genes identified with low AC and PC simultaneously. These individual rice genotypes became options for evaluation in the next generations.

Keywords: Rice (Oryza sativa L.), amylose content, chalkiness percentage, chi-square, genetic parameters, medium grain rice, molecular-assisted selection (MAS)

Key findings: In rice (Oryza sativa L.) F₂ populations, the parameters with PCV and GCV ≥ 20%, h2BS ≥ 60%, and GAM ≥ 20% were foreseeable for the rice quality traits, i.e., AC and PC for the selected cross. The Chi-square test of the AC and PC in the cross-population showed the segregated ratios of 3:1 in the phenotype and 1: 2: 1 in the genotype. The genotyping strategy employed in the study also proved cost-efficient and effective in terms of identifying the F₂ individuals carrying genes with low AC and PC.

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SABRAO Journal of Breeding and Genetics
56 (2) 681-694, 2024
http://doi.org/10.54910/sabrao2024.56.2.20
http://sabraojournal.org/
pISSN 1029-7073; eISSN 2224-8978

Date published: April 2024

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