E. PRASETYA, LAZUARDI, A. HASAIRIN, Y. RACHMAWATI, M. JANNAH, and T. HARSONO
SUMMARY
Cananga odorata (Lam.) Hook.f. & Thomson, a member of the Annonaceae family, has become widespread from the Indo-Malayan region to northern Australia and Malaysia. This plant has economic value because it produces essential oils used in the perfume and food industries. No research existed yet related to DNA barcoding to see intraspecific variations in C. odorata. This study aimed to analyze the potential of chloroplast genes maturase K (matK)-trnK, ribulose 1,5-biphosphate carboxylase (rbcL), and trnL-F intergenic spacer as DNA barcodes on C. odorata from North Sumatra, Indonesia. The research sample obtained from five areas included Tanjung Balai, Langkat Regency (Tangkahan Conservation Forest), Medan, Tanjung Morawa (Deli Serdang Regency), and Sukarasa (Deli Serdang Regency). Phylogenetic analysis using the three markers showed that C. odorata was monophyletic grouped from a common ancestor. The results of the phylogenetic tree construction also showed that the three markers used were able to group each genus in the Annonaceae family and form separate branches from the outgroup (Magnolia, Sarcandra, and Litsea). The barcoding gap on the genetic distance could not be observed in this study, although phylogenetic results showed that the three markers were discriminatory enough to separate species. The results showed that the matK-trnK, rbcL, and trnL-F markers could be effectively used as DNA barcodes to identify C. odorata.
Keywords: Cananga odorata, DNA barcoding, chloroplast genes, North Sumatra
Key findings: There are no barcoding gaps in the matK-trnK, rbcL, and trnL-F markers, although phylogenetic results show that three barcode markers are quite discriminatory to separate the Annonaceae species. DNA barcode matK-trnK, rbcL, and trnL-F revealed as efficient tools to identify C. odorata.
Date published: December 2022
DOI: http://doi.org/10.54910/sabrao2022.54.5.9
E. KURUCZ, A. ZS. ANDRÉ, M.G. FÁRI, M. SIPOS, and G. ANTAL
SUMMARY Random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR), and UPOV phenotype markers were used to study the DNA polymorphism in gamma-ray induced morphological mutants of Celosia argentea var. plumosa Hungarian variety ‘Arrabona.‘ In the experiments, the study determined the radio sensitivity and the genetic diversity of gamma radiation of C. argentea var. plumosa ‘Arrabona.‘ Seeds of C. argentea var. plumosa ‘Arrabona‘ were irradiated with gamma rays to increase their genetic diversity. The irradiation doses consisted of 0, 75, 150, 300, 450, and 600 Grays (Gy). The germination percentage, survival rate, and phenotype of irradiated plantlets underwent evaluation in the first (M1) and second (M2) generations. The investigation of genetic diversity used the ISSR and RAPD primers. Based on the results, the first-generation genetic distance increased as the doses increased. But the trend changed considerably through the generation due to the low condition and fertility of the high doses of gamma-irradiated plants. These individuals did not show at the next mutant generation, changing the population gene pool. In addition, open pollination has also changed genetic diversity. The RAPD and ISSR primers proved proper to evaluate the genetic diversity, nonetheless fewer direct connection occurred between the appearance and the used RAPD or ISSR markers. The LD50 dose between 150 and 300 Gray treatments and the radiation between 300450 Gray induced the median growth reduction in the mutant ‘Arrabona‘ population. Based on these results, the study concluded that both UPOV-based phenotyping and molecular marker analysis revealed appropriate for determining genetic divergence, but detecting greater genetic distance resulted in molecular markers.
Keywords: Celosia argentea var. plumosa, gamma irradiation, genetic variability, RAPD and ISSR primers, UPOV
Key findings: This paper determined the optimal gamma-radiation dose range to generate new C. argentea var. plumosa varieties. Results defined the LD50 and GR50 values as the best adequate data for predicting the irradiation effectiveness for mutant variant induction. The UPOV phenotypic determination method and the RAPD and ISSR primers determined the genetic distance within the M1 and M2 generations.
Date published: December 2022
DOI: http://doi.org/10.54910/sabrao2022.54.5.8
E.M. GINS, A.S. EGOROVA, A.B. SIVOLAPOVA, A.ZH. SEMENOV, KH.KH. APSHEV, A.A. MELESHIN, E.A. MOSKALEV, O.B. POLIVANOVA, G.L. BELOV, and S.V. GORYUNOVA
The wide occurrence of potato genotypes characterized by male sterility considerably complicates the realization of breeding programs. Therefore, male fertility in potato germplasm requires assessment as an important task. In the fresh study, 57 breeding lines from the Russian Potato Research Center and 85 cultivars of different origins underwent acetocarmine staining and in vitro germination assessment. With acetocarmine staining, 22 samples exhibited with tetrad sterility. In the remaining 120 samples, stained pollens vary from 0% to 88.7%, and germination varies from 0% to 19.4%. The pollen fertility assessment data obtained through acetocarmine staining and in vitro germination differed significantly. In the studied potato accessions, acetocarmine staining provided 18.3% sterile samples, while 50.7% by in vitro germination. The highest stained pollens (>80%) appeared in five potato accessions, i.e., 3004-7 (88.7%), 3000-32 (86.5%), 21.32-1 (81.8%), 17.32-1 (80.3%), and cultivar Garant (86.8%). In most potato accessions, the colored pollen grains ranged from 40% to 70%. The maximum percent of pollen germination occurred in the sample, 21.32-1 (19.4%). However, seven accessions (21.32-1, 18.5-17, 20.3-6, 20.33-8, and cultivars Edison, Queen Anne, and Garant) showed more than 10% pollen germination. During the growing season of 2022, the weather was hotter and drier compared with past long-term data, which could affect a decrease in the ability of pollens to germinate. The obtained results on male fertility will help in the study of the genetic control of pollen fertility and a further improvement in the breeding of Solanum tuberosum L.
Keywords: Potato (Solanum tuberosum L.), pollen fertility, male sterility, acetocarmine staining, in vitro germination, breeding techniques
Key findings: The results showed significant differences among the potato samples for pollen fertility. The 15.5% potato accessions characterized by the presence of tetrads were completely sterile. The pollen fertility assessment data obtained through acetocarmine staining and in vitro germination differed significantly. However, the acetocarmine staining can serve more useful for preliminary screening and validation of Solanum tuberosum accessions with tetrad sterility.
Date published: December 2022
DOI: http://doi.org/10.54910/sabrao2022.54.5.7.
A.N. FADHILAH, M. FARID, I. RIDWAN, M.F. ANSHORI, and A. YASSI
SUMMARY
Despite the increasing consumption of tomato (Solanum lycopersicum Mill.) in Indonesia, its yield capacity is lower than its demand. However, establishing high-yielding tomato varieties can overcome this. Strain in F2 populations is the first step in assembling high-yielding tomato genotypes through systematic selection, one through using a selection index. The latest study aimed to identify the genetic diversity and the effectiveness of the selection index for high-yielding F2 tomato population selection. The research took place from September to December 2021 at the Faculty of Agriculture Experimental Field, Hasanuddin University, Makassar, South Sulawesi, Indonesia. The study used an augmented design consisting of four blocks with complete randomization. Nine experimental units were used in this study, consisting of three F2 lines plotted into four blocks with no repetition and three cultivars that repeated in each block as genotype check. The study of 15 growth parameters used analysis of variance, correlation, and path analysis. Results revealed that the selection index proved efficient in selecting the F2 generation of tomato strain populations. Almost all the characters have the highest genetic diversity and showed potential for selection criteria usage. The total number of fruits (0.52), fruit diameter (0.32), and fruit weight (0.29) showed a direct influence on yield, and can serve as selection criteria for yield. The selection criteria were formulated into a selection index, producing 75 tomato strains potentially suitable as families in the F3 generation.
Keywords: correlation, genetic parameters, path analysis, selection criteria, selection index tomato (Solanum lycopersicum Mill.)
Key findings: Lines selection in F2 generation is critical in cultivar development, including the tomato (Solanum lycopersicum Mill.) crop. The study comprised the selection of promising tomato lines in F2 and consecutively in the F3 generation. The combination of several parameters to form a selection index on yield helped increase the effectiveness of selection. The selection index indicated 75 potential tomato lines for development in the F3 generation.
Date published: December 2022
DOI: http://doi.org/10.54910/sabrao2022.54.5.6
M. BABAR, M. ISHAQ, F. AKBAR, G. SUBKHAN, Z. ALI, M. ALI, B. ALI, K. KHAN, S. ALI, OBAIDULLAH, J. ALI, M.A. Qureshi, and H. KHAN
The research carried out under irrigated conditions during 2017–2018 estimated stress selection indices for morphological studies in wheat. Twenty wheat genotypes along with two checks (Pakhtunkhwa-2015 and Pirsabak-13) planted at Cereal Crops Research Institute (CCRI), Pirsabak Nowshera, Pakistan, had two sowing dates, i.e., regular plantation made on 24 November and late plantation on 25 December 2017, in a randomized complete block design with three replications. Nine stress tolerance indices, viz., tolerance index (TOL), mean productivity (MP), harmonic mean (HM), stress susceptibility index (SSI), stress intensity (SI), geometric mean productivity (GMP), stress tolerance index (STI), yield index (YI), and yield stability index (YSI) served as the basis for computation on each trait and every genotype under stress and non-stress conditions. Grain yield demonstrated a positive relationship with MP, GMP, HM, and STI under stress and non-stress conditions, which can serve as a better indicator for testing tolerant wheat genotypes. Overall, the maximum value of MP, HM, GMP, and STI, for days to heading displayed in genotype CDRI-PV-1: 113.00, 112.12, 112.56, 1.30; days to maturity in genotype PS-28 with 151.00, 149.24, 150.11, 1.28; for plant height in genotype PS-28 with 108.00, 107.94, 107.88, 1.27: for grain yield in cultivar PS-34 with 3983.89, 3958.96, 3934.18, 2.25, and for biological yield in genotype PS-23 with 11250.00, 10999.44, 10754.46, and 1.80, which specify that they are most stable and tolerant genotypes across both the planting conditions. Analysis of variance revealed highly significant differences among genotypes for most traits. Based on stress selection indices and mean performance in this experiment, PS-23, PS-33, PS-34, and PS-28 genotypes showed the best performance, and genotype Inq-91/FS(f8) resulted as the most stable performance and tolerance in stress conditions compared with other genotypes in stress selection indices. These genotypes could be beneficial in future wheat breeding programs to enhance the grain yield stability in bread wheat.
Keywords: Bread wheat, stress selection indices, stress and non-stress environments, optimum and late sowing, abiotic stress conditions.
Date published: December 2022
DOI: http://doi.org/10.54910/sabrao2022.54.5.5
W.U. KHAN, S.M.A. SHAH, H. ULLAH, I.H. KHALIL, S.A. JADOON, and D. WANG
SUMMARY
Water scarcity is a global dilemma, and rice crop needs plenty of water for optimum growth and yield. In the current climate change scenario, developing a broad-based gene pool of rice to help the crop breeders develop high-yielding cultivars needs dire action. This study assessed the genetic variation among 10 diversified parents and their 15 F5 populations developed under limited water supply for various morphological traits. Parents and F5 populations showed significant differences at 5% and 1% for most of the studied maturity and yield traits. F5 population ‘Dokri-Basmati/DR-92’ exhibited earliest for days to heading (93) with the highest culm length (85.5 cm). Maximum flag leaf area (34.67 cm2) resulted for the F5 population ‘DR-83/NIAB-IRRI-8.’ On the other hand, the F5 population ‘DR-83/DR-92’ excelled in performance for the number of primary branches panicle-1 (11). F5 population ‘IR-8/NIAB-IRRI-9’ displayed the longest panicle (28.70 cm) with the highest number of secondary branches panicle-1 (38). Three of the F5 populations ‘DR-92/DR-83,’ ‘DR-83/NIAB-IRRI-8,’ and ‘NIAB-IRRI-9/IR-8,’ displayed maximum heritability for panicle length (0.82), the number of primary branches (0.80), and secondary branches panicle-1 (0.94), respectively, offering the prospects for development of potentially high-yielding variety. The highest genetic advance for panicle length (9.87%) emerged from the F5 population ‘DR-92/DR-83,’ which also had the highest heritability for this trait. F5 population ‘DR-83/DR-92’ manifested maximum genetic advance (3.32%) for primary branches panicle-1, while ‘NIAB-IRRI-9/IR-8’ revealed the highest genetic advance (6.26%) for secondary branches panicle-1. Both of these populations may be suitable for developing the spreading type of rice germplasm with the potential water stress. F5 populations displayed differential responses for the studied traits, with none of the segregating populations excelling for studied maturity and yield traits. However, the germplasm pool created can serve as a better collection for improving existing populations from a production traits perspective under water-stress conditions or developing new cultivars focusing these traits for the target water stress region(s).
Keywords: Broad sense heritability, F5 populations, genetic advance, genetic variations, maturity traits, panicle traits, rice
Key findings: The study suggested that several genotypes have the potential for use in a breeding program for abiotic stresses, even if no genotypes showed as best for all the traits because of their diverse background. The germplasm can serve as material in the indigenous breeding program of rice and could also be available to other researchers as per a material transfer agreement for secondary breeding.
Date published: December 2022
DOI: http://doi.org/10.54910/sabrao2022.54.5.3
H. MATNIYAZOVA, S. NABIEV, А. АZIMOV, and J. SHAVKIEV
Summary
Water scarcity during the flowering and ripening stages disrupts physiological processes in crop plants. The recent study on cotton genotypes and their F1 hybrids under two different water regimes (nonstress and stressed conditions) took place in 2018–2020 at the Institute of Genetics and Experimental Biology, Academy of Sciences, Tashkent, Uzbekistan. In optimum and controlled water regimes, the cotton genotypes received irrigation four times, using 4800-5000 m3/ha water. However, under stress conditions, the genotypes received only two irrigations, using 2800-3000 m3/ha water. Comparing the optimum water regime with the water stress conditions, chlorophyll ‘b’ in plant leaves decreased by 3.0% to 46.7% and 1.1% to 26.2% in the parental cultivars and their F1hybrids, respectively. With water deficit conditions, the carotenoid content increased from 8.5% to 39.1% and 2.1% to 44.2% in plant leaves of parental cultivars and their F1hybrids, respectively, compared with the optimal water condition, which indicates how cotton genotypes protect themselves from water scarcity by varying magnitudes of carotenoids in plant leaves. The use of decreased levels of chlorophyll ‘a’ and ‘b’ and increased levels of carotenoids aided the inhibition of oxidants during photooxidation under drought conditions. The F1 hybrids viz., Listopad × Farovon (62.2±0.9 g.), F1 Kupaysin x Elastik (55.8±1.2 g.), F1 Listopad × Kupaysin (55.7±0.2 g.) produced higher seed cotton yield, which might be due to their resistance to drought conditions, as well as heterosis. These promising populations proved suitable for developing drought-tolerant cotton genotypes in future breeding programs.
Keywords: Gossypium hirsutum L., water regimes, drought conditions, physiological traits, chlorophyll, carotenoids, yield-related parameters
Key findings: The F1 promising populations, i.e., Listopad × Farovon, Kupaysin x Elastik, and Listopad × Kopaysin, showed resistance to drought conditions and gave higher heterosis and productivity under water deficit conditions.
Date published: December 2022
DOI: http://doi.org/10.54910/sabrao2022.54.5.2
M.F. ABBASI, A.U. DIN, and F.M. ABBASI
SUMMARY
A new rice strain that grows six feet tall with four times higher potential yield than the conventionally bred variety, JP5, was developed using the MAGIC approach. This new rice type underwent analysis on sink size and source capacity traits, including superior and inferior spikelets, vascular bundles of panicle neck and stem internode, tillering pattern, grain filling pattern, yield, and other morphological attributes. Results indicated that the new strain had more vascular bundles of the stem (42) and panicle neck (35), primary rachis branches of panicle (16.1), superior spikelets, and greater grain weight than the conventionally bred variety, JP5. The panicle measured 45 cm long, with fertile grains of 500 per panicle and a stem diameter of 1.2 cm. During the grain filling duration, the spikelets of this strain and superior spikelets of JP5 gained maximum weight earlier than the inferior spikelets of JP5. Six feet tall plants of this new strain with long and heavy panicles had greater stem wall thickness. There occurred a positive and significant correlation (0.97) between yield and small vascular bundles of the panicle neck, lumen diameter (0.98), leaf length (0.99), leaf width (0.99), flag leaf length (0.99*), flag leaf width (0.97<), panicle length (0.97), fertile grains per panicle (0.98), and plant height (0.97*). The study noted that improving sink size, source capacity, and transportation of assimilation contributed positively toward yield. This novel strategy for grain yield enhancement in rice proved beneficial for other cereals to get significant breakthroughs in their production for ensuring food security.
Keywords: Rice, sink size, source capacity, Abbasi strain, yield traits
Key findings: A new crop breeding methodology focusing on improved sink size, source capacity, and enhanced transportation of assimilate contributed toward the increased potential yield of newly developed rice strains. The strain could grow six feet tall with four-fold higher production than the conventionally bred variety, JP5. Com
Date published: December 2022
DOI: http://doi.org/10.54910/sabrao2022.54.5.1
SUMMARY
Increasing wheat production has become an urgent requirement to cope with rapid population growth and abrupt climate change. The management of phosphorus (P) and nitrogen (N) is one of the most important factors for sustaining and increasing wheat production, particularly in semiarid environments. This 2-year field study, which aimed to investigate the effect of P levels (0, 35, and 70 kg P2O5 ha−1) and N forms (urea, ammonium sulfate, and ammonium nitrate) on the yield attributes of three diverse highyielding commercial wheat cultivars (‘Shandawel-1’, ‘Sids-14’, and ‘Sakha-95’), was carried out at Om-Elzain Village, Zagazig, Egypt. The results indicated that increasing P levels was accompanied by a substantial increase in all evaluated traits, except spike length, and that high P level (70 kg P2O5 ha−1) was superior. Considerable genetic variation was detected among the evaluated cultivars for all studied traits. Cultivar ‘Sakha-95’, followed by ‘Sids14’, presented the most vigorous growth and enhancements in most yield components, grain yield, and biological yield. Furthermore, ‘Sakha-95’ recorded the highest agronomic P use efficiency, followed by cultivar ‘Sids-14’. N forms did not significantly affect all of the tested traits except plant height, spike number m−2, and 1000-grain weight, during both seasons. Ammonium-containing fertilizer (ammonium sulfate and ammonium nitrate) resulted in the highest values for these traits when compared with urea. Accordingly, ‘Sakha-95’ and ‘Sids-14’ are recommended for commercial use under high P levels. N forms had a marginally substantial effect on grain yield and its attributes.
Keywords: Phosphorus levels, nitrogen forms, wheat cultivars, grain yield and its attributes, agronomic phosphorus use efficiency, principal component analysis
DOI: http://doi.org/10.54910/sabrao2021.53.4.4
SUMMARY
Genetic engineering is one of the strategies for developing nitrogen (N)-use-efficient rice (Oryza sativa) varieties. One gene that plays an indirect role in N metabolism is alanine aminotransferase (AlaAT). It can efficiently increase N content and crop yield. In a previous study, the tomato AlaAT gene (LeAlaAT) was successfully isolated and introduced into ‘Mekongga’ rice. The present research was conducted during 2018 and 2019 at the Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), Bogor, Indonesia. The objectives of the present study were to perform the molecular characterization of LeAlaAT ‘Mekongga’ rice lines on the basis of the hpt marker gene, the direct PCR of the LeAlaAT fragment, and the phenotypic evaluation of the selected LeAlaAT T1 ‘Mekongga’ rice lines in response to different N fertilizer rates (0 kg ha−1 [control] and 60, 90, and 120 kg ha−1). This research involved three activities, namely (1) Southern blot analysis, (2) direct PCR, and (3) N use efficiency (NUE) test of ‘Mekongga’ transgenic lines. Southern blot analysis revealed that in T0 transgenic lines, the copy number of the hpt marker gene varied from 1 to 3. Direct PCR confirmed the presence of the AlaAT fragment in the T1 generation of five ‘Mekongga’ transgenic lines. The five transgenic lines showed high panicle number, biomass weight, shoot dry weight, and total grain weight under 120 kg ha−1 nitrogen. The high agronomical NUE of transgenic lines under 120 kg ha−1 N implied that the transgenic rice lines have the potential for efficient N use at a certain minimum level of N (120 kg ha−1 of nitrogen) and should be further evaluated at high N levels.
Keyword: Ma Alanine aminotransferase, LeAlaAT transgenic rice lines, Mekongga, Southern blot, direct PCR, N use efficiency
DOI: https://doi.org/10.54910/sabrao2021.53.4.14