A.I. POPOV, V.N. ZELENKOV , M.V. MARKOV, O.T. ZHILKIBAYEV, O.V. ROMANOV, E.V. SAZANOVA, G.D. KHOLOSTOV, K.I. TSIVKA, E.P. SHALUNOVA, J.V. SIMONOVA, and SONG GE
SUMMARY
The latest research proposed a new point of view about the material composition of so-called ‘humic substances,’ isolated from natural objects (soils, peats, sapropels, and composts) by alkaline solutions, and presented the conceptual model of direct influence of ‘humic substances’ on biochemical and biophysical processes into plants. ‘Humic substances’ are essentially black liquor, i.e., a product artificially obtained because of the alkaline hydrolysis of organic material from natural objects. Black liquor consists of a complex mixture of a variety of organic compounds. According to the proposed model, the biological activity of black liquor connects with accelerating the circulation of nutrients within plants, increasing the permeability of cell membranes, exhibiting de-toxicological properties, optimizing the ratio of organic and mineral anions in plants, and using some HS components as organic nutrients, inducing gene expression. Therefore, humic substances solutions have multifaceted effects on green vascular plants due to their direct influence on biochemical and biophysical processes.
Keywords: Humic substances, black liquor, alkaline hydrolysis, higher green plants, plant production process, colloidal systems of natural polymers, organo-mineral compounds, plant organic nutrients
Key findings: ‘Humic substances’ are the same as black liquor, artificially produced from the alkaline hydrolysis of organic material from natural objects. Black liquor composes a complex mixture of a variety of organic compounds. Hence, ‘humic substances’ solutions exhibit multifaceted effects on green vascular plants due to their direct influence on biochemical and biophysical processes.
Date of publication: October 2022
DOI: http://doi.org/10.54910/sabrao2022.54.4.21
E.M. GINS, S.M. MOTYLEVA, V.K. GINS, I.M. KULIKOV, and M.S. GINS
SUMMARY
The recent study aimed to investigate the composition of monosaccharides and their derivatives in the leaves of vegetable species Amaranthus tricolor L. cv. ‘Valentina’ of different ages, as well as, their vital role in enhancing the adaptive potential of the plant. Forty-eight monosaccharides and 28 of their derivatives have been identified in the composition of amaranth leaves of different ages. The maximum number of hydrophilic carbohydrate metabolites clustered in the young leaf. However, in older leaves, the number of water soluble metabolites showed similarities to that of the soluble in ethyl alcohol. Along with the general carbohydrate metabolites, the old leaves also contained specific monosaccharides, such as, lixopyranose, glucose, sorbose, mannobiose, cellobiose, and monosaccharide derivatives, i.e., methyl galactoside, glucopyranosiduronic and glucuronic acids, and alcohol erythritol. However, in Amaranthus tricolor L., the young leaf is characterized by arabinofuranose and carbohydrate derivatives, i.e., alpha-ketogluconic, arabinohexane, glucaric, galactaric, xylonic-D, lactone acids, and alcohol pentatriol. The leaves of different ages’ composition showed polyhydric alcohols (glycerin, ribitol, and myo-inositol). It also showed osmoprotective and antioxidant properties.
Keywords: Amaranthus tricolor L., cv. ‘Valentina,’ mono- and disaccharides and their derivatives, gas chromatography-mass spectrometry (GC-MS), antioxidants
Key findings: The gathered results show that the metabolism of Amaranthus tricolor L. leaves in different ages included general and specific monosaccharides. Their derivatives also exhibit antioxidant and osmoprotective properties.
Date of publication: October 2022
DOI: http://doi.org/10.54910/sabrao2022.54.4.20
S.A. ELSHAHAWY, S.A. NOMIER, F.S. MOHSEN, M.M. GAD, and R.A. KELANI
SUMMARY
Mango (Mangifera indica L.) is Egypt’s third major fruit crop. The latest study aimed to evaluate 11 foreign mango cultivars Kent, Palmar, Yasmina Rose, Shelly, Nam Doc Mai, Osten, Glenn, Sensation, Kensington Pride, Heidi, and Joa, in two successive seasons of 2018 and 2019, under Egyptian environmental conditions. The experiment comprised a randomized complete block design (RCBD) with 11 treatments and three replications to analyze the mango cultivars for floral aspects, fruit yield, its components, and fruit quality characteristics. Results indicated that cultivars Sensation and Yasmina Rose produced the highest fruit yield per tree. Cultivars Osten, Yasmina Rose, and Kent gained the maximum fruit weight. The study noted the uppermost value of fruit retention (%) for cultivars Palmar, Osten, and Joa. As for the initial fruit set, the highest value appeared in cultivars Heidi and Yasmina Rose, while cultivars Heidi and Kensington Pride revealed the highest value of fruit pulp firmness at the early stage of ripening in July. The Shelly cultivar recorded the highest percentage of pulp per fruit, while the lowest fruit fibers and total acidity percentage came from the Glenn cultivar. The fruits of Nam Doc Mai cultivar contained the highest total soluble solids and total sugar percentages, whereas fruits of the Heidi cultivar contained the highest value of vitamin C. Cultivars noted with the alternate bearing habit consisted of Kensington Pride, Palmer, and Shelly, while all other cultivars exhibited regular bearing. Mango cultivars Glenn, Nam Doc Mai, Osten, Kensington Pride, Shelly, Joa, Yasmina Rose, Sensation, Kent, Palmer, and Heidi (first mentioned, most recommended in descending order) received high recommendations for successful cultivation under the Egyptian environmental conditions based on relatively better fruit setting and quality.
Keywords: Mango (Mangifera indica L.), cultivars, evaluation, flowers, fruit yield, retention, fruit quality, fruit fibers and acidity
Key findings: The 11 mango cultivars exhibited varied differences in their characteristics. Reasons for these variations can be mainly due to their varied genetic makeup, as well as, the interaction between mango genotypes and the environment.
Date of publication: October 2022
DOI: http://doi.org/10.54910/sabrao2022.54.4.17
S.V. REZVYAKOVA, A.V. TARAKIN, N.I. BOTUZ, and E.V. MITINA
SUMMARY
This study comprises the selection of winter-hardy to highly winter-hardy pear (Pyrus CommunisL.) hybrid seedlings procured from various genetic origins. The development of pear hybrids resulted from intraspecific, interspecific, and distant hybridization. The simulation of the most damaging factors in the artificial climate chamber ensued according to the second and third components of winter hardiness – maximum frost resistance in a hardened state and stability of resistance to frost during the thaw. Critical temperatures affected the same seedlings, successively applying the specified modes, i.e., hardening at -10°C, freezing at -37°C (12 h), hardening at -10°C, thaw at +2°C (5 days), and freezing at -25°C (12 h). Combining genetically related variants to the highly winter-resistant cultivar, Phorun established the high breeding value for the yield of hybrid seedlings resistant to frost on the 2 + 3 components of winter hardiness. Thus, in the family 17-43-30 (Phorun – free pollination) × Shihan, 5.0% of transgressive genotypes came up, which withstood the given modes without damage to the buds and central tissues, showing 48.3% of highly winter-resistant forms. In a hybrid combination of 24-45-45 (Phorun × Olivier de Serre) × (Gervais + Olivier de Serre + Dekanka Zimnyaya), 33.3% of seedlings with frost resistance at the level of the highly winter-resistant control cultivar Tonkovetka attained selection. Remote hybridization opens up new opportunities in the pears’ selection for frost resistance. In total, and as a result of distant hybridization, 5.47% of the genotypes (with frost resistance for the 2 + 3 components of winter hardiness above Tonkovetka), 18.24% (at the level of Tonkovetka with freezing to 1.0 point), and 20.73% types (at the level of winter-resistant control cultivar Bessemyanka with freezing to 2.0 points) gained distinction.
Keywords: Pear, breeding, hybrid seedlings, frost resistance, winter hardiness components
Key findings: A comparative assessment was done on the winter hardiness of pear (Pyrus Communis L.) cultivars and their hybrids obtained through intraspecific, interspecific, and distant hybridization. New donors of winter hardiness and their hybrids received identification. Pear populations with highly winter-hardy traits gained selection from the genotypes of different genetic origins, i.e., Pyrus communis, P. ussuriensis, and P. bretschneideri.
Date published: October 2022
DOI: http://doi.org/10.54910/sabrao2022.54.4.16
V.N. GUSEV, SH.O. BASTAUBAYEVA, A.E. KHIDIROV, E.K. ZHUSUPBEKOV, and L.K. TABYNBAYEVA
SUMMARY
South and Southeast Kazakhstan underwent soil nitrogen forecasting and diagnosing for nutrition to establish the requirement of nitrogen fertilizers for field crops. The study established the possible use of alkaline hydrolyzable nitrogen as an indicator to provide field crops with available soil nitrogen. Managing the soil organic matter more accurately as the main source of nitrogen helps determine the spatial and temporal variability of its content in each specific field and to use a narrower (oriented to the prevailing soil types) scale of humus availability. The recent studies began in several stages, during 2014–2016, 2015–2017, and 2020–2021, on the irrigated light chestnut calcareous medium loamy non-saline and non-alkaline soil formed at the foothill loess plain of the Zailiyskiy Alatau, Kazakhstan. The nitrogen content of nitrates varied significantly over the years of research. In the control variants (without fertilizers), the recording of the minimum content of N-NO3 took place in 2016 compared with 2014 and 2015. In two consecutive years (2020–2021), 2,816 ha of arable land underwent testing in Almaty, Zhambyl, and Zhetysu regions, Kazakhstan. A total collection of 1,015 soil samples went through analysis, revealing a close correlation between the content of humus and alkaline hydrolyzable nitrogen in the upper soil layer of 0-30 cm. The hydrothermal conditions from a long multi-factorial field experiment data largely affected the intensity of mineralization and immobilization processes of the available nitrogen compounds. The rational use of nitrogen fertilizers makes it possible to increase the field crop yield with good quality and enhance the payback of applied fertilizers, maintaining and improving soil fertility.
Keywords: Light chestnut soil, humus, alkaline hydrolyzable nitrogen, the need for nitrogen fertilizers, the planned harvest
Key findings: Rational use of nitrogen fertilizers and the determination of spatial and temporal variability of nitrogen fertilizer consumption fully control the state of provision of plants.
Date published: October 2022
DOI: http://doi.org/10.54910/sabrao2022.54.4.15
S.K. SUDIRGA, I.M.S. WIJAYA, and A.A.K. DARMADI
SUMMARY
Anthracnose forms a group of fungal diseases that affect a variety of crop plants in warm and humid areas. Anthracnose causes the wilting, withering, and dying of tissues of plants, commonly infecting the developing shoots, leaves, and fruits. It critically affects the quality and production of Carica papaya fruits in Bali, Indonesia. The pathogenic fungi belonging to the genus Colletotrichum cause anthracnose fungal disease. Research on morphology and molecular identification of pathogenic fungi is very important to determine the type of pathogenic fungus that causes anthracnose disease in the papaya fruit. The latest study aims to identify the pathogen that caused anthracnose on papaya fruits in Bali, Indonesia, carried out during July – December 2021 at the Belok Village, District of Petang, Badung Regency, and in Laboratory of Biochemistry, Udayana University, Badung Regency, Bali, Indonesia. The study used Koch’s postulates to confirm the pathogenicity and continued with macro- and micro-morphological characterization. Based on macro- and micro-morphological characterization, the pathogen has shown similar traits to the genus Colletotrichum. Molecular identification took place using the ITS sequences to increase data robustness. Based on the molecular analysis, the DNA sequences of the genus Colletotrichum isolated from papaya fruits showed the symptoms of anthracnose disease. Based on the latest findings, the pathogen causing anthracnose disease in Carica papaya fruits showed as the species Colletotrichum magnum in Bali, Indonesia.
Keywords: Carica papaya, Colletotrichum magnum, anthracnose, molecular identification
Key findings: The latest study identified the pathogenic fungi (species Colletotrichum magnum) causing anthracnose disease on Carica papaya fruits through morphological and molecular approaches.
Date published: October 2022
DOI: http://doi.org/10.54910/sabrao2022.54.4.14
A.I. POPOV1, V.N. ZELENKOV, and M.V. MARKOV
SUMMARY
Drought is a severe causal factor of reduced crop yields than other abiotic stresses. Therefore, four sorghum genotypes underwent evaluation for their drought tolerance under three irrigation levels (100%, 70%, and 40% from evapotranspiration) under three sowing dates at the Higher Institute for Agricultural Cooperation Farm, Regwa region, Alexandria Desert Road, Egypt, to study the mean performance of grain yield plant-1 and transcriptomic analysis. The performance results reported that Shandaweel-6 and Hybrid-306 revealed the highest drought-tolerant hybrids, while Dorado and Giza-113 cultivars showed the lowest. The transcriptomic profiling of sorghum under normal and drought stress used the RNA-Seq method. Two differentially-expressed genes (DEGs) in leaves respond to drought. In the DREB2 case, the greatest average fold change showed 10.7 and 9.3 for the tolerant hybrids, Hybrid-306 and Shandaweel-6, respectively. Both genotypes performed significantly higher than the average fold change calculated for the sensitive genotypes Dorado and Giza-113 cultivars. This study contributes to a better understanding of the molecular basis of drought tolerance of sorghum and promotes sorghum improvement.
Keywords: Sorghum bicolor DREB2, CBF4, transcriptomic, cDNA, transcription factors, drought stress
Key findings: Sorghum hybrids Shandaweel-6 and Hybrid-306 received classification as drought-tolerant because of their best performance under drought stress and normal conditions. Both hybrids possess resistance genes for drought and other abiotic stresses. However, the cultivars Dorado and Giza-113 proved susceptible to drought stress due to their weak performances under drought-stress conditions.
Date published: October 2022
DOI: http://doi.org/10.54910/sabrao2022.54.4.13
A.M. HASSAN, M.F. AHMED, and M.A. RASHED
SUMMARY
Drought is a severe causal factor of reduced crop yields than other abiotic stresses. Therefore, four sorghum genotypes underwent evaluation for their drought tolerance under three irrigation levels (100%, 70%, and 40% from evapotranspiration) under three sowing dates at the Higher Institute for Agricultural Cooperation Farm, Regwa region, Alexandria Desert Road, Egypt, to study the mean performance of grain yield plant-1 and transcriptomic analysis. The performance results reported that Shandaweel-6 and Hybrid-306 revealed the highest drought-tolerant hybrids, while Dorado and Giza-113 cultivars showed the lowest. The transcriptomic profiling of sorghum under normal and drought stress used the RNA-Seq method. Two differentially-expressed genes (DEGs) in leaves respond to drought. In the DREB2 case, the greatest average fold change showed 10.7 and 9.3 for the tolerant hybrids, Hybrid-306 and Shandaweel-6, respectively. Both genotypes performed significantly higher than the average fold change calculated for the sensitive genotypes Dorado and Giza-113 cultivars. This study contributes to a better understanding of the molecular basis of drought tolerance of sorghum and promotes sorghum improvement.
Keywords: Sorghum bicolor, DREB2, CBF4, transcriptomic, cDNA, transcription factors, drought stress
Key findings: Sorghum hybrids Shandaweel-6 and Hybrid-306 received classification as drought-tolerant because of their best performance under drought stress and normal conditions. Both hybrids possess resistance genes for drought and other abiotic stresses. However, the cultivars Dorado and Giza-113 proved susceptible to drought stress due to their weak performances under drought-stress conditions.
Date of publication: October 2022
DOI: http://doi.org/10.54910/sabrao2022.54.4.12
N.G. TUMANYAN, M.A. ТKACHENKO, T.B. KUMEIKO, and S.S. CHIZHIKOVA
SUMMARY
Rice grain damage due to dark spots was noted more than 10 years ago in Russia. However, this phenomenon has long existed in other rice-growing countries. The factor limiting high rice yields are insect pests and pathogenic microorganisms, the vital activity of which leads to the occurrence of dark spots on the grain shells. This study hopes to assess the effects of dark spots on rice grains of six rice cultivars bred in Russia, i.e., Rapan, Khazar, Romans, Favorit, Trio, and Prestige. The study was carried out in 2020–2021 at the Federal State Budgetary Scientific Institution, Federal Scientific Rice Centre, Krasnodar, Russia. With an increase in the content of damaged grains from 5% to 10% and 20%, the mass of 1000 absolutely dry grains, vitreosity, and head rice content decrease, the filminess increased, and the content of damaged grains negatively affecting the quality traits of rice grains. If the content of damaged grains is up to 5%, grain quality decreases giving no impact of the parameter. The need to predict rice grain yield and quality based on grain damage intensity caused by dark spots ensures the profitability of growing various cultivars in rice production.
Keywords: Rice germplasm, seed dark spots, grain damage, grain yield, grain quality traits
Key findings: Damaged rice grains increase in the grain mass, decrease quality, grain size, vitreosity, and head rice content, and increase filminess. Overall, the content of damaged grains significantly affects the rice grain quality traits.
Date published: October 2022
DOI: http://doi.org/10.54910/sabrao2022.54.4.11
D.M. MAJEED, E.N. ISMAIL, F.R. AL-BURKI, A.S. ABED , and A.M.J. AL-JIBOURI
SUMMARY
Salinity is an abiotic stress factor and a major challenge that has significant negative effects on wheat production. It is also a source of concern for plant breeders leading them to reach reliable screening criteria for salt tolerance in wheat genotypes. The physiological analysis showed that the three salt-tolerant wheat genotypes viz., Dijla, 2H, and 3H showed the highest rate for the physiological traits i.e., chlorophyll content (38.9, 39.5, and 42.1, respectively),carbohydrates (600.14, 590.6, 560.8: 2H, 3H, and Dijla, respectively), proline acid (24.30, 23.14, and 21.87: Dijla, 3H, and 2H, respectively) under salt stress conditions, except protein percentage (3.8% and 3.3%: Rabia and Ibaa99, respectively) and K+/Na+ ratio (6.3 and 5.9: 2H and Dijla, respectively). The salt-tolerant wheat genotypes 2H, Dijla, and 3H enunciated an increased rate of expression of salt-related genes (TaOPR1 gene and β-actin gene) with values of 6.498, 4.0, and 3.768, respectively compared to two other salinity-sensitive cultivars i.e., Ibaa99 and Rabia under salt stress conditions. The salinity-sensitive cultivars i.e., Ibaa99 and Rabia showed no gene expression and significant difference with the control treatment after being treated with salinity stress conditions.
Keywords: wheat (Triticum aestivum L.), gene expression, TaOPR1 salt tolerance gene, salt-tolerant genotypes, chlorophyll, protein, carbohydrates, proline acid, K+/ Na+
Key findings: Under saline stress conditions, the genotypes of Dijla, 2H, and 3H showed the highest content of chlorophyll, protein (%), carbohydrate, and proline acid, except for K+/Na+ ratio, and excelled other wheat genotypes in gene expression (6.498, 4.0, and 3.768), respectively at the salt level of 16 dS/m. However, the salinity-sensitive wheat genotypes Ibaa99 and Rabia provided relatively low values for the expression of TaOPR1 gene expression associated with salt tolerance.
Date published: October 2022
DOI: http://doi.org/10.54910/sabrao2022.54.4.9