Genetic diversity and genetic gain are valuable parameters in plant breeding for assembling new cultivars. The presented study sought to identify the genetic diversity of the black soybean (Glycine soja L. Merrill) populations from crossbreeding and identify the broad sense heritability and genetic gain in yield-related traits. The set experiments proceeded in a randomized complete block design with three replications at two different environments/locations—Indramayu and Majalengka, Indonesia. The genetic material comprises 19 black soybean genotypes. The results showed that the genetic diversity in black soybean genotypes for grain yield and its attributes were broad, based on the principal component analysis, with a cumulative value of 80.55%. The environment greatly influenced heritability and genetic gains. High heritability and genetic gain were notable for seed length in the Majalengka location. In the Indramayu location, the demonstration of high heritability and genetic gains appeared for the number of fertile nodes and seed length. This information can serve as a basis for plant breeders to develop black soybean high-yielding cultivars in the future.

Keywords: Black soybean (Glycine soja L. Merrill), heritability, genetic gain, principal component analysis, yield related traits

Key findings: Information on genetic diversity, heritability, and genetic gain in yield-related traits is necessary to develop cultivars in crop plants. In black soybean populations, the genetic diversity for planting at two locations based on the principal component analysis was 80.55%. Heritability and genetic gain values among the populations for traits in two locations showed moderate to high values. This information can be beneficial in further selection to develop black soybean high-yielding cultivars.

The research investigates the diversity, bioprospecting, and conservation status of Pteridophyta in diverse habitats, focusing on the Bukit Tigapuluh National Park, Riau, Indonesia. The current research probes the diversity of pteridophytes and the conservation efforts of the Talang Mamak community in Riau, Indonesia, particularly in bioprospecting. It integrates ethnobotany and field exploration, utilizing ethnobotanical data as the primary database. Ethnobotanical data analysis used Microsoft Excel spreadsheets, incorporating respondent consensus factors and preference ratings. Conservation assessments adhere to the rigorous standards of the International Union for Conservation of Nature (IUCN) Red List Categories and Criteria. It uncovers 39 distinct Pteridophyta species belonging to 19 families. Engaging with the Talang Mamak community reveals nine plant species holding substantial bioprospecting potential, including Selaginella sp., Angiopteris evecta (G.Forst.) Hoffm., and Lygodium microphyllum (Cav.) R.Br. A comprehensive literature review underscores the scientific importance of these findings, highlighting bioactive compounds in 50% of identified species. Conservation assessments, rooted in the IUCN Red List Categories and Criteria, designate five Pteridophyta species as endangered. Pteridophytes play a vital role in the daily lives of the community, from traditional medicine to spiritual and cultural practices. This research underscores the imperative of holistic conservation strategies integrating scientific knowledge with community engagement.

Keywords: Polypodiaceae, species, biodiversity, bioprospecting, conservation, IUCN, medicinal plants

Key findings: The study achieved to compile the species diversity of ferns in Bukit Tigapuluh National Park (TNBT), Riau, Indonesia, comprising 39 species belonging to 19 families. In the Pteridophyta potential, nine identified species comprised Angiopteris evecta, one of the most prominent species. The latest findings provide a profound understanding of the potential of Pteridophyta and a solid foundation for further biosystematics and bioactive compounds exploration.

Climate change and enhanced air temperatures adversely affect the wheat’s (Triticum aestivum L.) morphological, physiological, biochemical, and molecular properties and grain yield. Therefore, developing high-yielding and resistant bread wheat cultivars to abiotic factors is vital, along with their primary source under varied environmental conditions. In the presented study, monitoring the influence of high temperatures (30 °С and above) continuously during the grain maturation period, especially in grain filling, on grain quality and productivity has undergone thorough research in the southern parts of Uzbekistan. These Southern regions mainly grow winter bread wheat cultivars. In determining the heat resistance of winter wheat cultivars typically planted in the Republic and selecting the best ones, the experiments and observations materialized with optimum and late-sowing conditions. The study determined the temperature during the growth period and daily temperatures during the grain-filling period. The research was able to evaluate the influence of air temperatures on growth phases, plant height (cm), spike length (cm), spikelets per spike, grain weight per spike, 1000-grain weight, grain nature, and protein and gluten content and compare the results under optimum and late periods.

Keywords: Bread wheat (T. aestivum L.), cultivars, climate change, high temperature, heat resistance, growth phases, grain-filling period, grain yield and quality

Key findings: In wheat (T. aestivum L.) cultivars with late sowing, the spiking stage began 9–10 days later than the optimum sowing. Grain yield decreased by 1.53– 2.76 t/ha and 1000-grain weight by 0.25–9.85 g due to a rise in temperature during maturation. Heat-susceptible cultivars manifested a sharp decline in their productivity.

Increased consumption of edamame soybean (Glycine max L.) needs supplementation by production boosts through crop area and productivity expansion utilizing high-yielding varieties. This research aimed to identify the genetic diversity of germplasm soybeans. This research transpired in Leuwikopo Experimental Farm, IPB University, using a single-factor randomized complete block design with three replications. This research evaluated 28 soybean genotypes consisting of edamame and grain soybean. The results showed a diversity in morphological characters at a value of 84.62% dissimilarity level with two main groups. The edamame soybean genotype, G.AGS 439 x L Tegal-1, and grain soybean Dega-1 gave the highest weight of pods (79.677 g and 75.596 g, respectively) and the best agronomic characters, making them potential parents in crosses for yield improvement. BioMax-1 and Grobogan genotypes could serve for quality improvement with high sugar, protein, and low-fat contents.

Keywords: Dissimilarity, edamame, nutritional analysis, quality

Key findings: This study provided a high genetic variation in the soybean’s morphological, agronomic, and nutritional properties. It nominated the potential genotypes of edamame soybean (Glycine max L.) with a high fresh pod weight, namely, G.AGS 439 x L Tegal-1 and Dega-1 as grain soybean, and the genotype of BioMAX 1 and Grobogan has favorable nutritional properties.

In the presented research, four recorded species of the genus Silene belonging to the family Caryophyllaceae attained as newly added species to science in the Iraqi flora. The current work involved the study of the morphological features of the vegetative and floral parts of these new species recorded in Iraq. The newly identified species were S. muscipula, S. nocturna, S. latifolia, and S. sedoides, belonging to the family Caryophyllaceae. The collected samples of the different landraces came from separate locations in the Northern Iraqi Mountains. The study also recorded information on locations, longitude, latitude, and collection dates. The diagnosis of four species and their naming relied on flora from neighboring countries, such as the Flora Iranica and Flora of South Africa. After confirmation, the description assessment of vital parts of the new plant species included the shape, leaf venation, length, shape, and calyx tube venation, the modification shapes, appendages of coronal scales petals, and pistil structures. The plant part images of the different landraces also enhanced the morphological characteristics of these new species under study.

Keywords: Caryophyllaceae, Silene, taxonomy, morphology

Key findings: The latest investigation studied the morphological characteristics of four newly registered species belonging to the genus Silene L., with the record of these new species also added to the Flora of Iraq.

Durio testudinarius is an indigenous durian species classified under the Malvaceae family, primarily distributed within the Borneo region. It has a specific feature of bearing fruit from the main trunk. Understanding its genetic diversity is crucial for its utilization. This study aimed to analyze the genetic diversity and phylogenetic relationships of five D. testudinarius accessions from West Kalimantan. A sample of D. testudinarius from five West Kalimantan populations, six Durio species (D. graveolens, D. acutifolius, D. lanceolatus, D. dulcis, D. kutejensis, and D. oxleyanus) and 17 Durio species NCBI dataset underwent genetic diversity and phylogenetic analysis using DNA barcoding markers from three chloroplast regions (matK, rbcL, and trnL-trnF intergenic spacer) and a nuclear DNA Internal Transcribed Spacer (ITS) region. The ClustalW alignment of the five D. testudinarius accessions revealed limited nucleotide variation in the chloroplast regions but significant variation in the ITS region, relating to genetic variation in the nuclear genome. Phylogenetic analysis showed that D. testudinarius is genetically more similar to D. beccarianus, which confirms that they flower on the trunk. This DNA barcoding data improves genetic libraries and assists conservation and breeding programs by revealing D. testudinarius and related species’ genetic variation.

Keywords: Chloroplast marker, ITS spacer, nucleotide variation, species phylogenetic

Key findings: This research offers valuable insights into genetic variation by providing DNA barcoding sequence data on four barcodes for D. testudinarius from West Kalimantan. This contribution enriches existing DNA libraries, supporting conservation efforts and plant breeding programs.

The oil palm (Elaeis guineensis. Jacq) is classifiable into two types based on its fruit color pigmentation—virescens, and nigrescens. Virescent fruits are green at the early stage, and then turn bright orange after ripening. Meanwhile, the nigrescent fruit color is dark purple during fruit formation and soon becomes red and purple after ripening. The heredity of virescent traits is favorable in detecting the ripeness of oil palm fruit bunches. The presented study determined the genetic structure characteristics of oil palms in the Nigerian and Angolan populations based on the Virescens (Vir) gene sequence. The 202 palm landraces used included 172 from Angola and 30 from Nigeria (1X and 5X). The young tissues of each palm served for DNA extraction. DNA analysis used a single fragment of a Vir gene, totaling 180 bp of DNA sequence. The results showed that a specific haplotype was prominent in the Nigerian and Angolan populations. The average genetic distance of the population was 0.0031 ± 0.0010. However, the highest genetic distance resulted in the Nigerian population 1X (0.0058 ± 0.0022). The lowest one was in the Angolan population (0.0024 ± 0.0012). The landraces were similar in several haplotypes; however, the Angolan population had more haplotypes than the Nigerian population. The Neighbor-Joining phylogenetic tree showed that landraces clustered based on their haplotypes, but this classification did not match the population.

Keywords: Nigerian and Angolan oil palm (E. guineensis), diversity, fruit color, genetic distance, Vir gene, DNA sequencing

Key findings: The results indicated that fruit color-specific single nucleotide polymorphisms (SNP) characterized the oil palm (E. guineensis) diversity; however, it did not form a cluster in the population.

The development of sorghum breeding must continue with an assessment of genetic parameters in developing sorghum lines. Hence, the presented study sought to estimate the genetic variation, heritability, and gain in the selected F3 populations obtained by crossing the sorghum parental genotypes ‘Bioguma-1’ and ‘Gando keta.’ The study transpired from June to October 2022 in the experimental field of Citayam Depok, Indonesia. Plant materials consisted of 160 lines from 18 selected genotypes in F2 using an augmented experimental design, with ‘Bioguma-1’ and ‘Gando keta’ as checks and parents. Based on performance, the selection of sorghum lines G01 (4-1), G02 (1-5), G03 (3-3), G04 (3-4), G05 (3-6), and G06 (3-8) proceeded for evaluation in the next generation for seed weight traits and similarity traits to cultivar Bioguma-1. The predicted genetic gain in the F4 populations will further enhance seed weight compared with the F3 populations and parental lines by 33.3% and, consequently, the superior sorghum genotypes for releasing as new cultivars with high yield components, broader stem and panicle diameter, and shorter plant.

Keywords: Bioguma-1, Gando keta, genetic gain, segregating populations, Sorghum bicolor

Key findings: The sorghum’s promising F3 lines revealed wide genetic variations and high heritability for plant height, wet and dry panicle weights, and seed weight traits. Several F3 lines selected for further studies in the next generation were on seed weight traits and their similarity to the parental cultivar Bioguma-1. In F4 populations, the expected genetic gain will further enhance seed weight compared with the F3 populations and parental lines and release them as superior sorghum cultivars.

Potato virus M (PVM) is one of the most widespread and economically damaging viruses worldwide, along with X, Y, S, A, and L. Therefore, outcomes of the monitoring surveys carried out in potato fields during this study showed numerous disease symptoms visible on potato plants, such as boat-shaped curling of leaf tips, mosaic twisting, leaf swelling, striped mosaic along the veins, and spotted mosaic. The diseased sample collections from potato plants and the molecular and genetic identification progressed based on the PVM protein coat (CP) gene. The results revealed that the boat-shaped curling and mosaic symptoms were PVM’s typical signs. The isolates’ identification continued through PCR product sequencing, and their submission in the NCBI database received a PP235793.1 tag and the name PVM-Uz-AY1. The phylogenetic analysis of the identified isolates showed that the said isolate was 98% related to the isolate GQ469609.1, and the rest to isolates, such as VF-Nevsky2 (LC511899.1), FE-Queen_anne10 (LC511893.1), and KC479342.1. Those located on other phylogenetic branches were KF561611.1, KF561603.1, and KF561608.1, which appeared to share 99% homology. In addition, the study identified the virus using the Real Time-PCR method, obtaining reliable results.

Keywords: Potato (Solanum tuberosum L.), PVM, isolate, RT-PCR, CP gene, PVM-Uz-AY1, primer, phylogenic analysis, Uzbekistan

Key findings: PVM-Uz-AY1 isolate of the PVM infecting potato (Solanum tuberosum L.) succeeded in identification using RT-PCR. The nucleotide sequence study of the CP gene responsible for this virus’ protein coat was intensive. The construction of a phylogenetic tree of the PVM-Uz-AY1 isolate occurred based on the bioinformatic analysis, determining the evolutionary origin of the virus.

The Barley Yellow Dwarf Virus (BYDV) is one of the most economically significant viruses from the Luteoviridae family that infects most crop plants of the family Poaceae. The presented study sought to molecularly identify BYDV in proso millet (Panicum miliaceum L.) and evaluate the genetic diversity of its CP (coat protein) gene concerning other strains worldwide. Plant samples collected from proso millet fields in the Navoiy Region, Uzbekistan, incurred BYDV-PAV RT-PCR for the CP gene. The results authenticated the presence of a BYDV-PAV strain in the collected samples. The identified nucleotide sequence of BYDV-PAV isolate T-UZB2 proceeded to deposit in the GenBank database. BLASTN analysis of this sequence showed the highest genetic similarity (98.75%) with the Pakistan PAV-type isolate PK3 (JX473288.1). Phylogenic analysis and multiple sequence alignment revealed that four mutation events (268G>A, 440A>C, 450A>C, and 464C>T) involving the BYDV-PAV isolate Pk3 Pakistan have considerably contributed to the evolutionary history of PAV T-UZB2 Uzbekistan haplotype. SMV identification can help control pathogens and reduce their impact on the soybean crop in Uzbekistan.

Keywords: Proso millet (Panicum miliaceum L.) survey, barley yellow dwarf virus (BYDV), RT-PCR, CP gene, phylogenic analysis, Navoiy Region, Uzbekistan

Key findings: The BYDV-PAV isolate detection in Uzbekistan occurred by RT-PCR in proso millet (Panicum miliaceum L.). The nucleotide sequence of the coat protein gene of this isolate reached recognition. Phylogenic analysis revealed its close relationship with the Pakistan PAV-type isolate PK3 (JX473288.1).