The application of entomophages and entomopathogens for biological control of harmful insect pests was the focus of this study, carried out from 2020 to 2024 through field experiments, laboratory analyses, and review of scientific literature in Uzbekistan. In total, four key biological control agents underwent assessment, such as Trichogramma chilonis, Coccinella septempunctata, Beauveria bassiana, and Metarhizium anisopliae, for controlling three major insect pest species (Helicoverpa armigera, Aphis gossypii, and Bemisia tabaci) across Uzbekistan’s multiple agroecological zones. The study comprised diverse environmental and cropping systems, including irrigated farmlands, semi-desert regions, and mountain foothills. Field observations revealed the combined application of entomophages and entomopathogens effectively reduced pest populations (by 65%–80%) compared with untreated control. Statistical analysis (ANOVA) confirmed these reductions were significant at the P < 0.05 level. The highest efficacy was notable during spring and early summer, corresponding with peak pest activity in cotton and vegetable crops. In addition to classical biological control methods, the study integrated precision monitoring and environmentally safe pest regulation techniques to enhance sustainability. The results contribute to the understanding of biological pest control ecology and support the development of modern and eco-friendly plant protection strategies in Uzbekistan and other similar agroecosystems.

Keywords: Biological control, entomophages, entomopathogens, Beauveria bassiana, Trichogramma chilonis, Integrated Pest Management (IPM)

Key findings: The combined use of entomophages and entomopathogens significantly reduced populations of major insect pests by 65%–80% across different agroecological zones of Uzbekistan, with the highest effectiveness observed in spring and early summer. The integrated application of Trichogramma chilonis, Coccinella septempunctata, Beauveria bassiana, and Metarhizium anisopliae proved to be environmentally safe and sustainable. These results confirm the high potential of biologically based and precision-supported pest management strategies within integrated pest management (IPM) systems.

This study evaluated the morphological and biochemical responses of potato genotypes to polyethylene glycol (PEG)-induced osmotic stress conditions. Using different concentrations of PEG solution assessed the adaptive response of genotypes in stress conditions. All potato genotypes exhibited reduced root and shoot length, a smaller leaf size, and decreased levels of photosynthetic pigments (chlorophyll a and b and carotenoids) under PEG treatments. However, an increase was evident in key indicators of oxidative and osmotic stress such as malondialdehyde (MDA), hydrogen peroxide (H₂O₂), and proline levels. Among the tested cultivars, Telman showed the highest sensitivity to PEG-induced stress, while the cultivar Ugur maintained relatively stable productivity and exhibited adaptive traits. Despite considerable initial growth, the cultivar Vagif displayed a weaker adaptive response under prolonged stress conditions. A significant negative correlation was notable among the proline, MDA, and pigment contents, while a positive correlation was between the stem diameter and pigment levels. Among the traits, the highest coefficients of variation revealed genotypic differences in stress responses. The results enunciated that PEG-induced osmotic stress affects the physiological traits differently across the genotypes, providing valuable insights for identifying drought-tolerant potato cultivars. The findings provide a sound base for future breeding programs aimed at developing drought-resistant potato cultivars.

Keywords: Potato (S. tuberosum L.), polyethylene glycol, malondialdehyde, proline, hydrogen peroxide, photosynthetic pigments, morphological and biochemical traits

Key findings: PEG-induced osmotic stress caused significant morphological and biochemical variations in potato (S. tuberosum L.) genotypes and revealed genotypic differences in drought response. The cultivar Ugur exhibited relatively stable adaptive traits, while the cultivar Telman was highly sensitive, and the genotype Vagif showed limited long-term tolerance. The results can provide a greater support in the selection of drought-tolerant potato genotypes

The study comprised a comparative evaluation of 29 accessions of alfalfa (Medicago sativa L.) in the control nursery and 21 accessions in the competitive nursery versus the standard cultivar Damu-12 during cropping seasons of 2023–2025 in Turkestan Region, Kazakhstan. Among the 29 accessions tested in the control nursery, 11 accessions (M-2515, M-2520, M-2521, M-2532, M-2579, M-2584, M-2587, M-2588, M-2562, M-2572, and M-2589) significantly exceeded the standard cultivar Damu-12 in green and dry matter yields. These accessions excelled the standard genotype by 110.2%–115.5%, achieving dry hay and green mass yields of 22.7–23.8 t/ha and 127.1–131.9 t/ha, respectively. In the competitive nursery with 21 accessions, 11 accessions (M-2572, M-2584, M-2532, M-2566, M-2537, M-2522, M-2579, M-2552, M-2582, M-2881, and M-2545) revealed the highest green and dry mass yields. Their green and dry mass yields outperformed the standard cultivar Damu-12 by 110.2%–122.9%. Cluster analysis categorized the accessions into three distinct groups: Cluster 1 (high-yield intensive type), Cluster 2 (high forage quality and dry matter), and Cluster 3 (stable/low performance). Clusters 1 and 2 were distinct as primary genetic resources for developing transgressive hybrids that combine rapid biomass accumulation with enhanced nutritional values.

Keywords: Alfalfa (M. sativa L.), breeding nursery, competitive varietal testing, stem height, leafiness, green mass yield, dry matter yield

Key findings: The relevant study identified the high-yielding irrigated alfalfa (M. sativa L.) genotypes through control and competitive nurseries. These promising accessions can be useful in future breeding programs for developing high-yielding cultivars for the Turkestan Region, Kazakhstan.

In 2024, at the emergence stage, the productive moisture reserves with chisel tillage (30–35 cm) were 241.8 mm vs. 232.4 mm with traditional plowing (20–22 cm). In the dry conditions of 2025, these indicators decreased; however, the chisel tillage provided higher values at the emergence stage (203.4 vs. 197.6 mm). The soil density was lower with chisel tillage (1.13–1.28 g/cm³) than with plowing (1.15–1.29 g/cm³), revealing a more stable agrophysical condition. With chisel tillage and plowing and no fertilizers in 2024, the barley yield was 2.83 and 2.61 t/ha, respectively, while in 2025, the yield was 1.31 and 1.21 t/ha, respectively. Application of mineral fertilizer (N₆₀P₆₀) provided an increase of 0.62–0.95 t/ha in 2024 and 0.36–0.44 t/ha in 2025, while the growth stimulator (Beres Amino Max) further enhanced grain yield (4.34 and 4.03 t/ha). The determination of grain yield formation came primarily from crop seasons (80.0%) and fertilizers (16.6%), with a lesser influence of soil cultivation (2.07%).

Keywords: Tillage methods, soil water-physical properties, spring barley (H. vulgare L.), grain yield, soil density, climate change, drought stress conditions

Key findings: The integration of chisel tillage with mineral nutrition and growth stimulator provided higher and more stable yields of spring barley (H. vulgare L.) than in traditional plowing, especially under water-deficit conditions and climatic instability.

In rice production, salinity is a major limitation requiring sustainable mitigation strategies to get the optimum yield. Practical approaches, such as the use of rice husk ash (RHA) as a silica-rich soil amendment and ascorbic acid as an antioxidant, are essential to enhance plant tolerance under saline conditions. This study aimed to evaluate the effects of salinity, RHA, and ascorbic acid on the morphophysiological and yield-related traits of the red rice cultivar Pamelen, carried out in 2024 at the Growth Center LLDIKTI I, Medan, North Sumatra, Indonesia. The experiment layout was in a factorial split-plot design with salinity levels (0, 4, and 8 dS/m), RHA dosages (0, 20, 40, and 60 g/plant), and ascorbic acid concentrations (0, 500, and 1000 ppm). Salinity stress levels significantly reduced rice growth and yield components, while chlorophyll content remained relatively stable. The application of RHA at 60 g/plant significantly improved vegetative growth, while the recorded highest grain weight per panicle was 40 g/plant. The treatment of ascorbic acid notably increased proline content, with the highest accumulation observed at 1000 ppm. Overall, RHA and ascorbic acid proved an effective ameliorant for reducing salinity-induced yield losses in red rice.

Keywords: Red rice, salinity stress, rice husk ash, ascorbic acid, morphophysiological traits, grain yield

Key findings: Salinity considerably reduced vegetative growth and development and grain yield of red rice. However, RHA substantially improved vegetative performance and grain formation, while ascorbic acid aided in proline-based stress adjustment. Chlorophyll remained stable across treatments, indicating maintained photosynthetic integrity.

This study aimed to identify irrigation strategies that optimize water use while maintaining favorable growing conditions for apple production and providing a reliable agronomic background for varietal evaluation. Field experiments took place during 2016–2018 on mature Golden Delicious trees grafted on MM106 rootstock. Comparing four irrigation treatments included drip irrigation, sprinkling, combined drip–sprinkling irrigation (drip at air temperatures ≤25 °C and sprinkling at >25 °C), and surface furrow irrigation (control). During the experiments, the soil moisture maintenance in the root zone was within the optimal limit. The study recorded data on microclimatic parameters (air temperature and humidity), plant water-regime indicators (leaf water content, transpiration intensity, relative turgidity deficit), yield, fruit size, sugar content (°Brix), and irrigation water productivity. The highest apple fruit yield emerged in the combined irrigation (about 19.7 t ha⁻¹), followed by sprinkling and drip irrigation, while surface irrigation produced the lowest yield. Drip irrigation required the least water and resulted in the highest fruit sugar content, whereas surface irrigation showed the lowest water-use efficiency and fruit quality. Overall, combined irrigation proved the most effective method in hot, low-humidity environments, providing a balance between water savings, yield, and fruit quality.

Keywords: Irrigation methods, water productivity, apple fruit yield and quality, sugar content, microclimatic conditions, water regime of plants

Key findings: Combined drip-sprinkling irrigation most effectively mitigated heat and humidity stresses, resulting in the highest water productivity and fruit yield of the apple cultivar Golden Delicious under arid conditions. Drip irrigation minimized water use and produced fruits with the highest sugar content, while surface irrigation was the least efficient.

The tomatillo (Physalis philadelphica) is a newly developed crop, gaining popularity as a tasty fruit with numerous medicinal properties in Vietnam. For tomatillo long-term cultivation, this study aimed to determine the effects of fertilizer combinations, plant spacing, and pruning on growth, yield, and fruit quality traits of tomatillo grown in An Giang Province, Vietnam. The results revealed the optimal fertilizer formula for promoting plant growth and development and quality traits of tomatillos, including the mineral-organic fertilizer ‘Dau Trau HCMK4,’ NPK (16-16-8+TE), and urea. The plant spacing of 70 cm × 60 cm helped increase stem diameter, leaf length and width, and fruit quality; however, it reduced plant height. Although plant pruning improved fruit diameter and size, it did not significantly affect the growth and yield-related traits. These data will aid in designing experiments and selecting appropriate cultivation methods for tomatillo to achieve higher and better-quality yields.

Keywords: Tomatillo (P. philadelphica), fertilizer combinations, plant spacing, pruning, growth and development, fruit yield and quality

Key findings: In cultivating tomatillo (P. philadelphica), the combination of organic and inorganic fertilizers considerably enhanced plant growth and development and fruit yield with better quality. Additionally, proper plant spacing and pruning also showed positive effects on plant growth and fruit yield.

Brassica juncea L. is one of the major oilseed crops in Pakistan and globally, with productivity sensitive to environmental variation. Forty-five B. juncea genotypes, evaluated across four locations over two years in Khyber Pakhtunkhwa, Pakistan, used a randomized complete block design with two replications. Significant (p ≤ 0.01) genotype and genotype × environment interaction (GEI) differences for seed yield and oil content led to genotype plus genotype × environment (GGE) biplot analysis to identify high-performing and stable genotypes across test environments. The GGE biplot view for mean vs. stability identified AUP-618, AUP-619, and AUP-623 for seed yield and AUP-625, AUP-626, and AUP-653 for oil content as high-yielding and stable genotypes. The polygon view revealed two mega-environments per trait, indicating crossover GEI. For seed yield, Peshawar and Mardan (2017-18) formed one possible mega-environment with AUP-600 and AUP-619 as winning genotypes, while Kohat (2016–17) and Bannu (2017–18) constituted the second with AUP-645. For oil content, Peshawar and Bannu comprised one potential mega-environment with AUP-645, whereas Mardan created the second with AUP-632. Furthermore, Mardan (2016–17) for seed yield and Peshawar (2017–18) for oil content emerged as the tentative ideal environments. GGE biplot analysis effectively identified high-yielding and relatively stable B. juncea genotypes for diverse Khyber Pakhtunkhwa environments.

Keywords: B. juncea, genotype-by-environment interaction (GEI), stability analysis, GGE biplot, seed yield, oil content

Key findings: The B. juncea genotypes AUP-618, AUP-619, and AUP-623 for seed yield and AUP-625, AUP-626, and AUP-653 for oil content succeeded in their identification as high-yielding and relatively stable genotypes. Two possible mega-environments, detected for both traits, indicated crossover GEI, with Mardan (2016–17) and Peshawar (2017–18) identified as the ideal environments for seed yield and oil content, respectively.

The succeeding study aimed to evaluate the influence of vanadium salt-based treatments on sugar accumulation, technological quality, and storability of sugar beet (Beta vulgaris L.) roots under the conditions of the Western Forest-Steppe of Ukraine. Field and storage experiments proceeded during 2022–2024, and the study included four different treatments of vanadium, i.e., control (no vanadium), low-dose foliar vanadium (0.25 L ha⁻¹, 50 g V L⁻¹), medium dose (0.5 L ha⁻¹), and high dose (1.0 L ha⁻¹). The vanadium application took place at the 6–8 leaf stage, and repeated treatment ensued before canopy closure. Roots harvested at full maturity sustained storage in field clamps for 120 days. The results showed sucrose concentration at harvest increased by 0.8%–1.5% in low and medium doses compared with the control (17.2% vs. 16.4%), while the higher vanadium dose caused a slight depression (16.0%). Storability showed a significant improvement, with losses of root mass and sucrose during 120 days of storage reduced from 14.5% (control) to 11.0% (medium dose). The excessive dose of vanadium caused a reduction in technological quality and increased root respiration rate. The results suggested that vanadium at optimal rates can enhance the sucrose yield and storability of sugar beet, although overdosing is detrimental.

Keywords: Sugar beet (B. vulgaris L.), vanadium salt, root mass, sucrose content, storability, Western Forest-Steppe of Ukraine

Key findings: The vanadium salt-based low dose (0.25 L ha⁻¹) significantly increased sugar beet (B. vulgaris L.) root yield and sucrose content compared with the control. Vanadium also improved storability and reduced sugar losses during postharvest storage.

The succeeding study comprised a comparative analysis of three-year research (2020–2022) on the fauna and ecological distribution of orthopteroid insects (Orthoptera, Mantodea, and Dermaptera) in the Korgalzhyn State Nature Reserve, Central Kazakhstan. Field surveys proceeded at 23 monitoring sites representing major habitat types, including wetlands, coastal zones, steppe, wormwood-grass communities, solonchaks, and stony biotopes. Overall, the recorded species totaled 46 belonging to eight taxonomic groups. The highest species richness resulted in the locust family Acrididae (25 species) and the bush-cricket family Tettigoniidae (12 species). Species distribution and abundance displayed considerable association with vegetation structure, moisture conditions, and the degree of anthropogenic impacts. In some habitats, population density reached up to 300 individuals per hour. The most widespread and dominant species were Chorthippus karelini, Euchorthippus pulvinatus, Chorthippus biguttulus, and Eremippus comatus, while the species Myrmeleotettix pallidus and Omocestus petraeus were dominating locally. An increase in the population of the potentially harmful species (Calliptamus italicus, Locusta migratoria, and Oedaleus decorus) also emerged. However, the documentation of several species occurred for the first time in the Korgalzhyn Reserve, including Roeseliana roeselii, Phaneroptera falcata, and Conocephalus dorsalis.

Keywords: Orthopteroid insects (Orthoptera, Dermaptera, Mantodea), Korgalzhyn reserve, ecological distribution, biodiversity, population, steppe stations, fauna of Kazakhstan

Key findings: With three years of study, identifying 46 species of orthopteroid insects succeeded in the Korgalzhyn Reserve, demonstrating the highest biodiversity across steppe and wetland habitats. Dominant species, such as Chorthippus karelini and Euchorthippus pulvinatus, revealed considerable adaptability to variations in vegetation structure and moisture conditions.