Maman is one of the food plants growing in Riau Province, Indonesia. It has the names joruk maman and African cabbage, typically eaten as a fermented vegetable. Its verification as Cleome gynandra L. synonym Gynandropsis gynandra by the matK and trnL-trnL-trnF intergenic spacer (IGS) used the DNA barcode sequences. The latest research aimed at molecular authentication of maman based on rbcL and ITS sequences and the evaluation of locus combinations (rbcL+ITS and rbcL+ITS+matK+trnL-trnL-trnF IGS). The DNA extraction utilized a modified CTAB method and phylogenetic analysis using MEGA11. The rbcL and ITS sequences were 839 and 672 bp in length, respectively. The results revealed the maman plant from Riau was distinct as C. gynandra / G. gynandra based on single- and multi-locus analyses. The results suggested a unique nucleotide was evident in the rbcL sequence in the maman from Riau. All the single and multilocus can be applicable as DNA barcodes for C. gynandra and G. gynandra.
Maman plant from Riau, Cleome gynandra, Gynandropsis gynandra, ITS, rbcL, DNA barcode, phylogenetic analysis
DNA barcoding is a useful technique for molecular verification in crop plants. The presented results could prove vital for researchers in recognizing maman plants (C. gynandra and G. gynandra) from Riau in the future.