Tamarix L. (Tamaricaceae) is a taxonomically challenging genus. Six Iraqi species of Tamarix include T. amplexicaulis L., T. aphylla L. Karst., T. arceuthoides Bunge, T. gracilis L., T. mascatensis Bunge, and T. ramosissima Ledeb, which incurred evaluation for genomic diversity and taxonomic identities. The following study presents an analysis of the taxa from all previously described Tamarix species using nuclear and plastid DNA markers on fresh samples of Tamarix species. DNA genomic isolation proceeded by the ABIO pure extraction protocol. This study amplified one specific PCR fragment partially covering transposable pBS ISS to assess the pattern of genetic polymorphism in Tamarix species. Building a specific tree assessed the accurate genotyping of the observed variants and their phylogenetic distribution. Researchers focused on GenBank-searched sequences as DNA barcodes for a meta-analysis. Rectangular and circular cladograms explored displayed the different representations of integrated Tamarix sequences. The presented investigations revealed many interesting facts. This study pursued sequencing to reconstruct the phylogeny by integrating the sequencing results with robust evidence for the species classification and developing molecular markers based on ISS analysis of Tamarix conducted in Iraq.

Keywords: DNA barcodes, DNA sequences, phylogenetic analysis, ISS analysis, Tamarix, species, taxonomy

Key findings: According to ISS, the Iraqi Tamarix species’ isolation occurred by matching with GenBank-searched sequences as DNA barcodes for a meta-analysis. The highlighted details of their sequences had the total length of the amplified amplicons determined. These results were greatly analogous to earlier observations based on the phylogenetic analyses that supported the accurate placement of these isolates within their respective clades and correcting past phylogenetic findings on the relationships among Iraqi Tamarix species.