Author Archive Kaye1214

SABRAO JOURNAL of BREEDING and GENETICS Volume 50 Number 3 SEPTEMBER 2018

Phenotypic variability in cowpea (Vigna unguiculate L. Walp) genotypes assessed with quantitative and qualitative characters

Onwubiko NC

Phenotypic variation among six Nigerian cowpea genotypes was established on the basis of quantitative and qualitative characters. Except for leaf area and harvest index, quantitative characters showed significant differences. Stem and pod color and leaf and seed color showed variation among genotypes. Seed size had the least variation. Quantitative and qualitative characters were useful for assessing morphological variability and discriminating cowpea accessions.

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Onwubiko NC (2020). Phenotypic variability in cowpea (Vigna unguiculate L. Walp) genotypes assessed with quantitative and qualitative characters. SABRAO J Breed Genet 52(2):191–201.

Genetic Diversity of Cucumis and Mukia (Cucurbitaceae) based on ISSR markers

Pratami MP, Chikmawati T, Rugayah

Published: June 2020

A total of 53 Cucumis cultivars and 43 Mukia accessions were verified through genetic diversity analysis based on inter simple sequence repeat markers. The amplification of Cucumis and Mukia DNA by using 20 ISSR primers produced 246 bands and 245 polymorphic bands. Mukia javanica (Miq.) C. Jeffrey (0.101) showed the highest diversity, whereas Mukia maderaspatana L. (0.037) showed the lowest diversity. Principal component analysis grouped Cucumis and Mukia accessions into four groups.

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Pratami MP, Chikmawati T, Rugayah (2020) Genetic diversity of Cucumis and Mukia (Cucurbitaceae) based on ISSR markers. SABRAO J Breed Genet 52(2):127–143.

Agronomic characters and seed protein content of soybean (Glycine max [l.] Merr.) Lines across environments

Kuswantoro H, Ginting E, Yuniarti N, Artari R, Rahajeng W, Purwatoro, Nugrahaeni N

Published: June 2020

The agronomic performance and seed protein contents of 49 soybean lines were investigated in two environments. Location, genotype, and genotype × environment interactions influenced the investigated characteristics. The agronomic characteristics and protein content of the 49 soybean lines varied between locations, and protein and methionine content varied across genotypes.

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Kuswantoro H, Ginting E, Yuniarti N, Artari R, Rahajeng W, Purwatoro, Nugrahaeni N (2020). Agronomic characters and seed protein content of soybean (Glycine max [l.] Merr.) Lines across environments. SABRAO J Breed Genet 52(2):91–108.

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SABRAO JOURNAL of BREEDING and GENETICS Volume 50 Issue 2 June 2018

Evaluation of Cowpea Cultivars from Southwet Maluku-Indonesia under Different Environments based on Morphological Characteristics

R.L. KARUWAL1,2, SUHARSONO3*, A. TJAHJOLEKSONO3, and N. HANIF4

      1Study Program of Plant Biology, Graduate School, Bogor Agricultural University, Bogor 16680 Indonesia
      2Study Program of Biology Education, Pattimura University, Ambon 97233 Indonesia
      3Departement of Biology, Bogor Agricultural University 16680, Indonesia
      4Departement of Chemistry, Bogor Agricultural University, Bogor 16680, Indonesia
      *Corresponding author’s email: sony-sh(at)apps.ipb.ac.id
      E-mail addresses of coauthors: karuwalritha8(at)gmail.com; aristj(at)apps.ipb.ac.id, nhanif(at)apps.ipb.ac.id

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Summary

Cowpea is one of the crops that have a lot of potential to be utilized as raw food resource in the Southwest Maluku district. The objective of this study was to evaluate local cowpea cultivars from Southwest Maluku under different locations based on morphological characters. The research was conducted in Ambon and Bogor using randomized complete block design (RCBD) with three replications. As many as seven local cultivars and three cultivars from ILETRI were evaluated. Observations were conducted on 18 morphological characters. The results showed that morphological characters were significantly affected by cultivars on each location. In general: KM3, KM4, and KM6 cultivars have the highest value in some morphology characters. Correlation analysis showed a significant correlation between the number of nodes with number of leaves followed by seed number plant-1 with seed weight plant-1 and loci number pod-1 with seed number pod-1 . Principal component analysis showed that there were nine principal components and clustering in two clusters. The local cultivars have the highest genetic potential in this study and could be selected as plant materials in future plant breeding program.

Key words: Different environments, local cowpea, Southwest Maluku, morphological characters

Key findings: Cowpea cultivar KM3 was the best genotype at both locations i.e., Ambon and Bogor, Indonesia. It also showed similar stability in some characters and almost equal with cultivars from ILETRI in these locations.

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Comparison of Yield Components of Sugarcane Varieties Grown under Natural Short- and Long-term Water-logged Conditions in Thailand

CH. PALACHAI1, P. SONGSRI1,2 and N. JONGRUNGKLANG1,2*
1Department of Agronomy, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand
2Northeast Thailand Cane and Sugar Research Center, Khon Kaen University, Khon Kaen 40002, Thailand
*Corresponding author’s email: nuntawootjrk(at)gmail.com
Email addresses of coauthors: patcharinso(at)kku.ac.th, chuleeporn_p(at)kkumail.com

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Summary

Against the background of a changing climate, water logging is a major problem for sugarcane production and results in reduced productivity. Various sugarcane genotypes cultivated in waterlogged areas show different acclimations of yield component traits. Understanding such traits under these conditions could be useful in the selection of suitable sugarcane cultivars. Therefore, the objectives of this study was to evaluate cane yield and millable cane, stalk weight, stalk length, and sugar yield of 12 sugarcane varieties in upland (short water logging period) and lowland (long water logging period) areas. Yield, sugar yield, stalk length, stalk diameter, millable cane, and weight per stalk were measured at 12 months after planting. Under both conditions, the tested sugarcane genotypes were differed in terms of yield, millable cane, single stalk weight, stalk length, stalk diameter, and sugar yield. Long water logging periods induced cane yield reduction and decreased single stalk weight. KK3, Kps01-12, and TBy28-0941 showed consistently high productivity across short and long water logging conditions. A positive correlation between single stalk weight and cane yield was existed, and this trait could be used as criteria selection for high productive cultivars under flooding conditions. Millable stalk number could also be used as a surrogate trait under these conditions.

Key words: Single stalk weight, millable cane, stalk length, flooding, sugar yield

Key findings: The twelve sugarcane genotypes used in this study were significantly differed in terms of yield, yield components, and sugar yield under short-term and long-term water logging conditions. Long periods of water logging result in lower cane yield, sugar yield, single stalk weight and stalk length compared to those obtained under short-term water logging. Single stalk weight contributes to high cane yield and could be used as a selection characteristic for improving cane productivity under water-logged field conditions.

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Genetic Analysis of Grain Yield of F4 Populations for Developing New Type of Upland Rice

R. HERAWATI*, MASDAR and ALNOPRI
Crop Production Department, Faculty of Agriculture, University of Bengkulu, Indonesia
*Corresponding author’s email: reny.herawati70(at)gmail.com, reny.herawati(at)unib.ac.id
Email addresses of coauthors: masdar.msdr(at)gmail.com, alnopri_bkl(at)yahoo.co.id

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Summary

High production of rice is closely related to high yield component characters namely the number of filled grains per panicle or the density of grains per panicle. These characters are complex and greatly determine yield. These traits are also controlled by many genes whose expression is influenced by environmental conditions. This research aims to study genetic diversity and inheritance patterns of rice yield characteristics in the F4population and to obtain the best genotypes from the selection. The materials in this research were 190 F3 generation seed numbers from the pedigree selection which consisted of 24 field numbers resulting from single crosses between local varieties (Bugis and Sriwijaya) with both IR7858-1 and IR148 (N22) that are tolerant to drought. Our research used an augmented design with four parents as check varieties. The research showed that the grain yield of F4 populations was polygenic and controlled by additive gene actions. The heritability value and coefficient of genetic diversity for grain yield were classified as moderate and high. The intensity of differentials selection by 10 percent based on the grain weight/hill, increased the middle value of other observed characters, like panicle length by 20.9%, the total number of grain by 48.4%, the filled grain number per panicle by 59.7%, and grain weight/hill by 40.9%. However, it decreased percentage of empty grain/panicle by 87.6%. Selection on higher filled grains and dense panicles, regardless of panicle length should be considered for developing the new plant type for upland rice with high yield.


Key words: Grain yield, F4 population, heritability, genetic variability

Key findings: Studying the inheritance systems of characters to form the desired character is an important step in segregating populations. Selection based on high grain yield in the F4 generation is expected to be significant in the development of high yield new type of upland rice.

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Genetic Diversity in Jackfruit based on Molecular Characters in Indonesia

D. Palupi1, S.S.B. Rahayu2 and B.S. Daryono2*

      1Faculty of Biology, Jenderal Soedirman University, Indonesia
      2Faculty of Biology, Gadjah Mada University, Indonesia
      *Corresponding author’s email: bs_daryono(at)mail.ugm.ac.id
      Email addresses of coauthors: dianpalupi08(at)gmail.com, ssbudirahayu7(at)yahoo.com

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Summary

Jackfruit (Artocarpus heterophyllus Lam.) is one of the most widely cultivated fruit in Indonesia. The Faculty of Forestry, Gadjah Mada Universtity, Indonesia has 11 provenances where jackfruitis widely grown in Indonesia. The aim of this study was to assessed variation injackfruit based on molecular marker RAPD (Random Amplified Polymorphic DNA). Seven RAPD primers namely A-13, A-27, B-4, H-15, L-1, OPB-1, and OPD-19 generated 70 DNA bands, of which 40 were polymorphic. In this study H-15 primers produced more polymorphic fragments than the other six primers and had the highest percentage of polymorphic fragments, namely 91.7%. Similarity matrix was calculated using coefficient of Jaccard. Unweighted Pair Group Method Using Arithmetic Mean (UPGMA) cluster analysis was performedto develop a dendogram. This data analysis was performed by NTSYS software ver. 2.1. In the present studies, jackfruit tress accessions from the eleven provenances were divided into two clusters with similarity coefficient ranging from 63.33 to 85.71%, provenance of East Java and West Java were the most closely similar (85.71%) based on molecular characters.

Key words: Jackfruit, molecular characters, genetic diversity, RAPD

Key findings: Detection of genetic differences and relationship between jackfruit from 11 provenances of Indonesia for sustainable cultivation and for conservation of plant genetic resources.

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Morphological Characters Variability of Taro in Kalimantan, Indonesia based on Phenetic Analysis Approach

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L. Oktavianingsih1,2,* E. Suharyanto3 B.S. Daryono4 and Purnomo5

      1Postgraduate Student, Faculty of Biology, Universitas Gadjah Mada, Indonesia
      2Department of Biology, Faculty of Mathmetics and Natural Science, Universitas Mulawarman, Indonesia
      3Laboratory of Plant Structure and Development, Faculty of Biology, Universitas Gadjah Mada, Indonesia
      4Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada, Indonesia
      5Laboratory of Plant Systematics, Faculty of Biology, Universitas Gadjah Mada, Indonesia
      *Corresponding author’s email: oktavianingsihlinda(at)yahoo.com, linda.oktavianingsih(at)ugm.ac.id
      Email addresses of coauthors:haryantobiougm(at)ugm.ac.id bs_daryono(at)mail.ugm.ac.id, purnomods(at)ugm.ac.id

Summary

Taro (Colocasia spp.) is an aroid plant which has long been cultivated in Kalimantan. This plant is considered as one of the oldest forms of cultivated plants in the region. Information about its morphological variability is needed to develop taro breeding population as future alternative crop. The aim of this research was to determine the morphological characters variability of taro in Kalimantan, Indonesia based on phenetic analysis approach. Leaf, petiole, stolon and corm samples were collected in all Indonesian provinces of Kalimantan. Similarity index was measured using the gower general similarity coefficient and cluster analysis was carried using the unweighted pair group method using arithmetic average (UPGMA). A dendrogram and principal component analysis (PCA) were constructed using Multivariate Statistical Program v.3.1 software. Around 123 accessions had been collected and evaluated using 39 morphological traits. Taro in Kalimantan consists of C. esculenta, C. affinis, Colocasia sp and Colocasia wild type. C. esculenta is the most widely used as staple food by local residents in Kalimantan. It has two botanical varieties. Fifty two from all the accessions are local cultivars that developed from C. esculenta varieties. Cluster analysis revealed Coefficient Similarity of taro ranging from 0.615 to 0.974, and divided into two major clusters. The first cluster has Colocasia sp as its single subgroup, while the second cluster has six subgroups including C. esculenta, C. affinis and Colocasia wild type. In Kalimantan, taro leaf especially petiole colour has significant influent in taro grouping.

Key words: Colocasia spp, Kalimantan, morphology, phenetic, taro, variability

Key findings: This research provides information about the characteristics of taro that are useful for plant breeding programmes and for developing taro as an alternative crop.

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