SUMMARY
Against the background of global climate change, drought stress has become one of the environmental limiting factors that can significantly influence the growth and development of crop plants. Drought stress conditions also cause changes in plant physiological and metabolic processes. The influence of soil drought on the mineral composition of the leaves of two Actinidia species with С3-type photosynthesis, namely, Actinidia argutа (Siebold & Zucc.) Planch. ex Miq. cultivar ‘Taezhny Dar’ and Actinidia kolomikta (Maxim. & Rupr.) Maxim. cultivar ‘Narodnaya’, was studied through energy dispersive spectrometry. The investigations were carried out during 2020 to 2021 at the Department of Genofonde and Bioresources of Plants, Federal Scientific Center for Horticulture, Moscow. The present research revealed that actinidia leaves contained the following major elements: K (11.19 mass% to 13.84 mass%), Ca (7.83% to 12.08 mass%), Cl (6.20 mass% to 7.33 mass%), and Mg (2.98 mass% to 3.44 mass%). Low values were recorded for Mo (1.19 mass% to 4.49 mass%) and P (0.83 mass% to 1.25 mass%). In both species, the mineral elements K and Ca were present at high levels. A positive correlation was observed between Mg–P, KMn, Mn–Se, Cu-Se, P–Si, Na–Mo, and Si–Mn in the leaves of A. argutа and between Cl–Ca, Mo; P–Si, Mo; and K–Ca in the leaves of A. kolomikta. Under stress conditions, the ratios of K/Ca and K/P declined to 0.9 and 6.3, respectively, whereas those of K/Cl, K/Mg, and K/Mo increased to 3.8, 4.4, and 2.7, respectively. The present studies confirmed that actinidia leaves contained high concentrations of minerals, especially K, Ca, P, and Mg, and that the accumulation of mineral elements in actinidia plant leaves under drought conditions varied depending on the species.
Keywords: Mineral composition, leaves, drought stress, EDS analysis, Actinidia argutа, Actinidia kolomikta
DOI: https://doi.org/10.54910/sabrao2021.53.4.13
SUMMARY
Mangroves are well-adapted halophytes that thrive in coastal saline environments. They live under difficult environmental conditions, such as high light intensity and external salt concentrations, as well as low-oxygen environments, such as water-logged muck, that are typically inappropriate for the survival of other plants. Salinity is a major abiotic factor that affects plant growth, productivity, and dispersal in tropical and semitropical intertidal areas. Furthermore, it affects approximately 20% of all cultivable land and 50% of all irrigated land on the planet. Mangroves have developed a sophisticated salt filtration mechanism and a complicated root structure to withstand salty water exposure and tidal movement. The expression patterns of five salt tolerance genes (amFer1, amDhna, amSod1, amCat1, and amUbc2) in the Egyptian gray mangrove (Avicennia marina Forssk.) grown under different environmental conditions in South Sinai protectorates (Nabq, Ras Mohamed, Safaga, and Wadi El-Gemal), Egypt, were investigated in this study. This study aimed to assess and examine the genetic behavior of mangroves in response to salinity by using quantitative real-time PCR. Findings revealed differences in the expression patterns of the investigated genes under various conditions, showing that salinity influences plant genetic response. Ferritin gene expression was high in all locations, indicating that ferritin represents an essential component of the mangrove response mechanisms.
Keywords: Mangrove (Avicennia marina), (amFer1, amDhna, amSod1, amCat1, and amUbc2), salt tolerance genes, quantitative real-time PCR
DOI: https://doi.org/10.54910/sabrao2021.53.4.11
SUMMARY
Rice is not just a staple food; but it is at the center of culture, politics, and economy of Bangladesh. The determinants affecting the adoption of modern rice cultivars and their adaptability in the Aman season (June to November) at the Faridpur region in Bangladesh were discussed. A total of 500 farmers from five different districts such as, Faridpur, Madaripur, Shariatpur, Rajbari, and Gopalganj through multistage sampling technique participated in the survey. Before the survey, 15 farmers were selected for the cultivation of eight modern T. Aman rice cultivars in their fields during three consecutive years 2018 to 2020 in the mentioned five districts of Bangladesh. A structured questionnaire was used to identify the determinants associated with the adoption of modern rice cultivars by replacing the popular old ones. Results revealed that beyond preferences, some other determinants (age, education, risk aversion, land size, yield, and perception of modern rice cultivars and their seed availability) directed the decision to adopt or not. Considering the average grain yield of the tested rice cultivars, the genotypes BRRI dhan72 (G-6) and BRRI dhan87 (G 8) were the high yielding cultivars for the above five districts. Despite having a comparatively low yield, the cultivars BRRI dhan71 (G-4) and BRRI dhan49 (G-3) were more stable compared to other cultivars in the Faridpur region of Bangladesh.
Keywords: Adoption, adaptability, heritability, GGE, Aman rice
DOI: https://doi.org/10.54910/sabrao2021.53.4.9
SUMMARY
The biochemical compounds of red raspberry (Rubus idaeus L.) fruits cultivated with conventional growing technology and on a nutrient substrate were studied during 20192020 at the Federal Horticultural Research Center for Breeding, Agrotechnology and Nursery, Moscow, Russia. The antioxidant activity, phenolic compounds, and ash constituents of the fruits and the metabolites of the alcoholic extract of the raspberries were determined. The effect of growing technologies, i.e., conventional vs. nutrient substrate, on the accumulation of macro- and microelements in raspberry fruits was established. In red raspberries grown on nutrient substrate, the antioxidant activity decreased by 25 times (aqueous extract) and 1.5 times (alcoholic extract). The K and Na contents and Se contents of red raspberries grown on nutrient substrate were 1.5 and 3 times higher than those of raspberries of grown with conventional technology. Raspberries grown with conventional technology contained 2 times more Ca, Ni, and Mn and 7.4 times more Fe than raspberries grown on nutrient substrate. The total amount of elements in raspberries grown through soilless cultivation was 5.5% higher than that in berries grown conventionally. A total of 48 compounds were identified in the alcoholic extracts, and only 29 substances were found in berries grown on a nutrient substrate. Sugar and citric acid constituted the largest share of red raspberry components. Fructose and turanose disaccharide synthesis in raspberries grown on nutrient substrate was 20% higher than that in conventionally grown raspberries. A total of 48 organic compounds with different biological activities were identified. They included five substances with antimicrobial activity, three phenolic substances, eight organic acids, four sugar acids, nine amino acids, and 19 sugars and their derivatives. At the same time, 42 compounds were found in raspberries grown with traditional technology, and 21 compounds were identified in raspberry fruits grown on nutrient substrate. Three fatty acids, namely, ɑ-linoleic acid (polyunsaturated omega-6 fatty acid), palmitic acid, and stearic acid (saturated fatty acid), along with cinnamic acid, shikimic acid, and chrysin were found in berries grown conventionally.
Keywords: Rubus idaeus L., conventional growing, nutrient substrate, nutrients, antioxidant activity, ash constituents of fruits, metabolites, bioactive compounds
DOI: https://doi.org/10.54910/sabrao2021.53.4.8
SUMMARY
Submergence stress due to unpredictable soil flooding is one of the mainconstraints encountered in rainfed growing areas, especially in Southern Sumatran riparian swamps. The development of submergence-stress-tolerant cultivars through the introgression of Sub1 via marker-assisted backcrossing (MABC) is an ideal solution. This study was carried out during 2020 at Sriwijaya University, Palembang, Indonesia, with the aim to select Sub1-introgressed lines in BC3F1 generations on the basis of MABC and to screen out the SSR markers that were unlinked to the target gene for application in subsequent background selection studies. Results revealed that almost all the backcrossed progenies segregated from the rice parental cultivars ‘FR13A’ and ‘Pegagan’. The backcrossed lines showed significantly improved submergence stress tolerance and recovery rates compared with their parents. Sub1 introgression into the BC3F1 generation was confirmed by the tightly linked Sub1 marker SUB1C173, and marker RM23915 was used for recombinant selection. These markers followed the expected marker segregation ratio in accordance with the Mendelian single gene model. In the parental polymorphism survey, 84 out of 237 SSR markers that were unlinked to the target loci were found to be available for background study. Twenty-seven backcrossed lines were selected on the basis of foreground selection. Seven plants were selected on the basis of the recombinant marker RM23915. Five backcrossed lines were further selected on the basis of submergence stress tolerance and agronomic performance.
Keywords: Backcrossing, SSR markers, Sub1 gene, submergence tolerance, Oryza sativa L.
DOI: https://doi.org/10.54910/sabrao2021.53.4.3
SUMMARY
Potato (Solanum tuberosum L.) is one of the most important vegetable crops and the fourth most important edible crop after the three major cereal crops. It is considered as an approximately complete diet food because of its nutritional value. Its center of origin is Peru, South America. In Punjab, Pakistan, the districts of Sahiwal and Okara serve as the core areas of potato cultivation. Thirty-three potato genotypes were collected from the core areas of Punjab for characterization. The genetic diversity of potato germplasm was assessed on the basis of morphophysiological traits. This experiment was conducted with a randomized complete block design and three replications. The data on 14 morphological and physiological traits were recorded. Analysis of variance indicated the presence of highly significant variation for each physiological and morphological trait. Correlation analysis showed that plant yield was highly correlated with the number of tubers per plant (0.484), tuber weight (0.648), and chlorophyll contents (0.365). By contrast, tuber dry matter exhibited a significant highly negative association with tuber moisture content (−0.753). Algorithmic hierarchical cluster analysis allocated the genotypes into four distinct clusters. Cluster 2, which was the largest cluster, comprised 18 genotypes. By contrast, cluster 4 was the smallest cluster and contained only two genotypes. The results of diversity analysis obtained through hierarchical clustering were further validated through principal component analysis (PCA). PCA provided five significant principal components that contributed 72.39% of the total variation. The principal components of the biplot explained 41.95% of the total variation, with tuber moisture content and tuber dry matter as distinct traits. Cultivars ‘SH5’, ‘SH 7-18’, ‘Simply Red,’ and ‘Ruby’ were the vertex genotypes in the biplot. Results indicated the prevalence of significant variation in the tested germplasm. Furthermore, the assessment of diversity at the molecular level is recommended for the further validation of genetic diversity.
Keywords: Genetic diversity, Punjab, morphophysiological traits, principal component analysis, hierarchical clustering, Solanum tuberosum L.
Nihad T.A. AL-JANABI, and Mushtaq T.H. AL-ZURFI
SUMMARY
The latest study focused on snapdragon (Antirrhinum majus L.) plants during the fall season of 2021–2022 in a greenhouse at the Agricultural Division, University of Kufa, Najaf, Iraq. The study aimed to determine the response of snapdragon plants to foliar application of arginine (0, 20, 40, and 60 mg L-1) and nano-iron (0, 30, 60, and 90 mg L-1). The experiment comprised a randomized complete block design with a factorial arrangement and three replications. The arginine (60 mg L-1) foliar spray led to a significant enhancement in the flower and physiological traits, i.e., flower carrier length (19.62 cm), age of the flowering inflorescence (25.01 days), leaf content of carbohydrates (12.75 mg g.-1) and iron (48.93 mg kg-1), and flower content for anthocyanin dye (21.40 mg 100 g-1) compared with the lowest values of the control treatment viz., 13.88 cm, 16.70 days, 11.25 mg g-1, 15.22 mg kg-1, and 9.82 mg 100 g-1, respectively. The nano-iron (90 mg L-1) also significantly improved the flower and physiological traits, i.e., the flower carrier length (18.06 cm), age of the flowering inflorescence (22.75 days), leaf content for carbohydrates (12.16 mg g-1); iron (38.08 mg kg-1), and flower content for anthocyanin dye (16.72 mg 100 g-1) compared with the control, i.e., 15.79 cm, 20.02 days, 11.71 mg g-1, 29.61 mg kg-1, and 13.05 mg 100 g-1, respectively. The interaction of arginine (60 mg L-1) and nano-iron (90 mg L-1) provided a positive effect of their individual application for flower and physiological traits (22.55 cm, 27.93 days, 12.93 mg g-1, 50.04 mg kg-1 ,and 23.06 mg100 g-1) compared with the control (12.84 cm, 15.43 days, 10.84 mg g-1, 13.0 mg kg-1 ,and5.13 mg 100 g-1), respectively.
Keywords: Snapdragon (Antirrhinum majus L.), arginine, nano-iron
Key findings: The single and dual interaction foliar application of arginine (60 mg L-1) and nano-iron (90 mg L-1) significantly improved the flower and physiological traits of snapdragon (A. majus L.) plants, followed by the individual application of arginine (60 mg L-1) and nano-iron (90 mg L-1).
Date of publication: October 2022
DOI: http://doi.org/10.54910/sabrao2022.54.4.23
A.J. MOHAMMED and Ali.S.A. AL-JANABI
Citrus production faces many problems; a major one consists of irrigating citrus seedlings grown at Agricultural Research Stations and various nurseries with saline well water, which determines the growth, reproduction, and spread of citrus in Iraq. The said problem needs addressing to reduce its effects on the growth and reproduction of citrus fruits. Therefore, the latest research aimed to determine the effects of regular liquefied water and saline well water, in addition to a foliar spray of aspartic and ascorbic acids, on the growth and physiological properties of the grafted orange seedlings. The local citrus seedlings of the same age and size were selected and used as the original and grafted with local orange buds. They were shifted and tested for the above three factors at the Horticulture and Forestry Division, Najaf Agriculture Directorate, Iraq. The findings showed that irrigation with regular liquefied water had improved the growth traits, i.e., plant height, leaves plant-1, leaf area plant-1, and dry biomass weight of sweet orange seedlings, compared with saline well water. Results further revealed that foliar application of aspartic acid (100 mg L-1) positively affected the leaves plant-1 and dry biomass weight compared with the control. The ascorbic acid (4000 mg L-1) application gave highly superior and well-responsive reactions for most of the traits, i.e., leaves plant-1 (69.3 leaves plant-1), leaf area plant-1 (3888 cm2), dry biomass weight (0.3629 g), and total chlorophyll content (7.03 mg 100 g-1 fresh weight) in orange seedlings.
Keywords: Sweet orange transplants, aspartic, ascorbic acids, water quality
Key findings: Salinity impacts agriculture and crop plants must be induced for salt tolerance and sustained economic output. Antioxidants, such as, aspartic acid and ascorbic acid have auxinic action and also have a synergistic effect on plant growth and salinity tolerance.
Date of publication: October 2022
DOI: http://doi.org/10.54910/sabrao2022.54.4.22
T. HUSSAIN, Z. AKRAM, G. SHABBIR, A. MANAF, G. RABBANI, J. IQBAL, U. JAVED, R. AHMAD and J. AKHTAR
SUMMARY
Chickpea (Cicer arietinum L.) is negatively affected by drought stress at all the growth stages, including germination and seedling emergence. The recent study aimed to investigate the drought-tolerant Kabuli chickpea genotypes at the seedling stage under osmotic stress conditions. An in vitro screening technique evaluated the 120 different genotypes of Kabuli chickpea for drought tolerance by using different concentrations of polyethylene glycol (8000) solution, viz., T0: Control, T1: -0.19 MPa, and T2: -0.47 MPa, arranged in a completely randomized design. Data were recorded on germination percentage, root length, shoot length, seedling length, root/shoot ratio, dry matter, seedling vigor index, and proline content. The results of a pooled analysis of variance revealed significant variability among genotypes, different polyethylene glycol treatments, as well as, between genotypes and treatments. The increase of polyethylene glycol levels negatively affected most of the parameters under study, except for proline content, which increased with an increase in polyethylene glycol concentration. The study results indicated that the advanced lines, 15KCC-106, 13KCC-114, 6KCC-103, GP-37, FS-10, and 12KCC-106, performed better under different osmotic stress conditions and gained selection as drought-tolerant advanced lines at early seedling stage. These advanced lines suitably combine with their desirable traits to cope with the drought condition and can serve as a baseline for the improvement of Kabuli chickpea breeding material for drought tolerance.
Keywords: Kabuli chickpea,drought tolerance, polyethylene glycol, proline
Key Findings: The breeding lines, 15KCC-106, 13KCC-114, 6KCC-103, GP-37, FS-10, and 12KCC-106 were identified as drought tolerant at the seedling stage and will be used in future breeding programs for the development of drought-tolerant Kabuli chickpea genotypes.
Date of publication: October 2022
DOI: http://doi.org/10.54910/sabrao2022.54.4.19
T.H. TAMRAZOV
SUMMARY
The present research discusses the dynamics of changes in the surface of assimilation organs of different durum and bread wheat genotypes differing in the maturation period due to drought conditions. Under drought conditions, the soil water potential decreases, and in the later stages of the plants, the turgor pressure decreases, the stomata close, and a sharp decrease in photosynthetic activities. The situation creates stress in the crop plant, and various biochemical, physiological, and molecular reactions arise to overcome and protect itself from stress, allowing the plant to develop resistant mechanisms to adapt to the external environment. The existent research aimed to determine drought effects on assimilating surface areas and productivity traits of durum and bread wheat genotypes differing in their maturation periods grown under various climatic and soil conditions, with the comparison of the physiological indices. The experiments proceeded on wheat genotypes with contrasting maturation periods (early, medium, and late) during 2021–2022 at the Research Institute of Crop Husbandry, Absheron, Azerbaijan. The assimilating surface area of various organs underwent comparative studies in two durum (Garagylchyg-2 and Alinja-84) and two bread wheat (Nurlu-99 and Gobustan) cultivars under normal water supply and drought conditions. Post anthesis water stress caused a 34% and 27% reduction in grain yield and 1000-grain weight, respectively, while no significant effect on the grains per spike and spikes per m2. The averages of grain yield and 1000-grain weight of different wheat cultivars in the controlled condition showed 696±36g/m2 and 43.1±0.8 g/m2, respectively, while under water-stress conditions, these values significantly decreased to 452±57 g/m2 and 31.6±1.4 g. The significant reduction in grain yield due to post-anthesis water stress might be due to a reduction in photo-assimilates production.
Keywords: wheat genotypes, the drought factor, duration of ripening, assimilation area
Key findings: Resistant cultivars’ determination resulted from the effect of drought on productivity indicators against the background of changes in the main physiological characteristics of durum and bread wheat genotypes that differ in terms of maturity.
Date of publication: October 2022
DOI: http://doi.org/10.54910/sabrao2022.54.4.18