Tag Archive maize

SABRAO JOURNAL of BREEDING and GENETICS Volume 50 Number 3 SEPTEMBER 2018

This September 2018 issue covers articles on rice, maize, soybean, mango, shallots, olive, and cereals.

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VOLUME 49 ISSUE 3; September 2017

On this volume, studies are on maize, rice, wheat, peanut, Karanda, and mangosteen.

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SABRAO Journal Volume 48 Issue 2 June 2016

In this issue, articles are on wheat, cassava, maize, indian mustard, eggplant, rice, rape seed, etc.

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SABRAO Journal Volume 47 Issue 4 December 2015

This final issue for 2015 contains articles on tomato, jatropha, rice, maize, linseed, pea, lentil, fenugreek, rice, etc

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SABRAO Journal Volume 47 Issue 3 September 2015

This issue for 2015 has articles on sesame, okra, cotton, rice, barley, bitter gourd, maize and Indian mustard in Sudan, Myanmar, and India.

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SABRAO Journal Volume 47 Issue 1 March

This first issue for 2015 has articles on cabbage, wheat, corn, tomato and coriander.

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Genetic diversity among the Philippine traditional maize (Zea mays L.) populations based on SSR markers.

P.R. GUEVARRA, J.F. PARIL, R.R. GARDOCE, A.M. SALAZAR, and A.O. CANAMA-SALINAS

Summary

This paper reports the first genetic diversity analysis of Philippine traditional maize populations performed through a cost-effective DNA pooling strategy. The diversity among selected 100 traditional maize populations collected from Luzon, Visayas, and Mindanao was evaluated using twenty simple sequence repeats (SSR) markers at the Institute of Plant Breeding, University of the Philippines Los BaƱos, Laguna, Philippines. A total of 138 bands ranging from two to 12 bands per primer were detected. The average number of polymorphic alleles, polymorphism rate, effective multiplex ratio, marker index, resolving power, and expected heterozygosity are 6.283, 87.17%, 5.798, 4.104, 15.897, and 0.658, respectively. The polymorphism information content (PIC) varied between 0.141 to 0.848, with an average value of 0.620. A dendrogram was constructed with a dissimilarity coefficient ranging from 0.14 to 0.55 and a mean dissimilarity index of 0.425. Cluster analysis revealed 13 groups based on the result of Approximately Unbiased (AU) p-values from 10,000 bootstrap iterations. The cluster analysis enabled the classification of populations with ambiguous places of origin. Analysis of molecular variance (AMOVA) showed higher within-population diversity (70%) than among-population diversity (30%) with PhiPT (pairwise genetic differentiation metric) of 0.298 (P = 0.001). These results revealed the significant diversity of traditional maize populations in the Philippines and the power of SSR markers in diversity and cluster analyses despite the age of this marker technology. These findings will aid plant breeders in developing approaches towards knowledgeable and efficient execution of breeding programs using traditional maize populations.

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Date published: September 2022

Keywords: Traditional maize, Philippine germplasm, cluster analysis, SSR markers, AMOVA, bootstrapping

DOI: http://doi.org/10.54910/sabrao2022.54.3.1

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