PRODUCTION OF MICROSHOOT USING THE TEMPORARY IMMERSION SYSTEM (TIS) IN POTATO CULTIVARS

PRODUCTION OF MICROSHOOT USING THE TEMPORARY IMMERSION SYSTEM (TIS) IN POTATO CULTIVARS

L.A.M. SIREGAR, I. SAFNI, S. ANDRIANI, and S.P. HERNOSA

Citation: Siregar LAM, Safni I, Andriani S, Hernosa SP (2023). Production of microshoot using the Temporary Immersion System (TIS) in potato cultivars. SABRAO J. Breed. Genet. 55(6): 2227-2238. http://doi.org/10.54910/sabrao2023.55.6.33.

Summary

The Temporary Immersion System (TIS) Bioreactor’s performance in cultivation for several commercial potato cultivars became the prevailing study’s review focus. The experiment in a split-plot design had two factors. The first factor comprised the type of culture system (conventional and TIS Bioreactor) treatment used as main plots. The second factor was the four potato cultivars (Atlantic Malang, Dayang Sumbi, Granola L., and Maglia) used as subplots. The TIS Bioreactor culture has the highest average in all studied variables compared with conventional cultures at the multiplication stage. In the TIS Bioreactor, potato cultivar Dayang Sumbi excelled in the number of axillary branching and number of primary roots. At the same time, genotype Granola L. surpassed the height of the plantlets of other cultivars. Meanwhile, the cultivar Atlantic Malang shone in the number of nodes, with Maglia in the number of leaves. Using the TIS Bioreactor produces microshoots with more nodes and can be beneficial as a propagation organ. The Dayang Sumbi cultivar was superior to all other potato cultivars.

Potato (Solanum tuberosum L.), cultivars, conventional in vitro, TIS Bioreactor, microshoots, axillary shoots, number of nodes

The Temporary Immersion System (TIS) Bioreactor can be advantageous as an alternative to the cultivation system to maximize the productivity of plantlets and microtubers of several potato cultivars, which is beneficial. The success of increasing the axillary branching and the number of internodes through the TIS system will encourage an increase in the production of microshoots as one of the primary seed sources.

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SABRAO Journal of Breeding and Genetics
55 (6) 2227-2238, 2023
http://doi.org/10.54910/sabrao2023.55.6.33
http://sabraojournal.org/
pISSN 1029-7073; eISSN 2224-8978

Date published: December 2023

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