The presented study comprehensively assessed barley (Hordeum vulgare L.) hybrid populations of F₃–F₅ generations, comparing with the standard barley cultivars, Karagaydinckiy-5 and Astana-2000. The crossing of isolated barley cultivars of the international collection (obtained from Australia) proceeded under intense continental climatic conditions of Northern and Central Kazakhstan. Barley promising selected populations, i.e., Macguarie × Arna, Flinders × Tselinniy golozerniy, and Flinders × Omskiy golozerniy, showed early maturity (79–83 days), superior plant height (34.4–69.5 cm), and enhanced 1000-grain weight (56.6 g, 56.4 g, and 58.0 g, respectively), and populations, viz., Buloke × Karagandinckiy-6, Fathom × Donezckiy-9, and Onslow × Karabalykckiy-43, for productivity (1 m²) at 184 g, 116.4 g, and 140.1 g, respectively. Identified in the study were the correlation of productivity and its structural elements, particularly the grain weight per ear (r = 0.486) and grain weight per plant (r = 0.828), mainly determining grain productivity. The determination of structural features variation showed a significant excess (more than 20%) with varying levels. The level of variability of grain mass per plant has shown in hybrid lines, i.e., Fathom × Karagandinckiy-5, Onslow × Karagandinckiy-10, Admiral × Karabalykckiy-150, and Admiral × Donezckiy-9. In grains, the protein content ranged from 10.45% to 16.63%, and the excess over the standard cultivar resulted in the hybrid lines Franklin × Sabir (16.63%), Anodolu-86 × Donezckiy-8 (16.04%), and Flinders × Omskiy golozerniy (15.31%). Based on an average of the study years, the drought-resistant and high-productivity hybrid lines were Buloke × Karagadinckiy-6, Fathom × Donezckiy-9, Onslow × Karabalykckiy-43, Onslow × (Karagandinckiy-5 × Аrna), Bass × Karabalykckiy-150, Granal × CMB93H-805-F-1Y-1M-OY-17TRS-OAP, and Granal × CMB89A-380-1M-OGH-105GH-1B-1OY-OAP-19AP-OAP. These promising genotypes can benefit the development of drought-resistant and high-yielding barley cultivars through future breeding programs under prevailing environmental conditions.

Keywords: Barley (Hordeum vulgare L.), hybrids, breeding, drought, traits association, yield-related traits, protein content

Key findings: The study based on hybrid populations of world genetic resources of barley (Hordeum vulgare L.) under existing environmental conditions makes it possible to select and develop drought-resistant and high-yielding spring barley cultivars through the conventional breeding program and provide food security to the Republic of Kazakhstan.

Cassava leaves’ consumption as vegetables are common in several Asian countries. They contain various nutrients, such as, anthocyanins, carotene, minerals, and vitamins. New varieties with high mineral contents in leaves need development to increase the leaves’ quality as vegetables. This study aimed to identify and characterize cassava genotypes of the MV10 generation resulting from gammaray-induced mutations based on the leaves’ morphology, growth characteristics, and mineral contents, using two cultivars and 10 cassava genotypes. All genotypes planted in a randomized complete block design had three replications. The leaf’s morphology and mineral contents underwent scrutiny on the third to fifth leaf below the apical shoot. The mineral content analysis used the Atomic Absorption Spectrophotometry method. Data analysis comprised the ANOVA test and descriptive analysis. This study revealed petiole length, leaf lobe length, and leaf lobe width were significantly different between genotypes at four months after planting (MAP). However, no meaningful difference showed for the plant height, stem diameter, and number of leaves among genotypes. In general, the leaf morphology consisted of purplish green apical leaves with present pubescence; the shape of the central leaflet is lanceolate; the petiole color is reddish to greenish; leaves are dark green with five to seven leaf lobes; leaf veins are reddish-green; smooth lobe margins; and horizontal petiole orientation. The genotypes affect mineral contents, as the genotype with the highest Mg contents was G6-2-15-5-3, and the one with the highest Zn contents was G2D1-422. Fe contents showed more variations between genotypes, and no genotype showed consistently high Fe contents. This research produced promising genotypes for Mg or Zn contents in leaves for future cassava varieties for vegetable production.

Keywords: magnesium, mutant putative potential, iron, leaves, zinc

Key findings: This study nominated the potential mutant genotype with a high Mg content, G6-2-15-5-3, and a high Zn content, G2D1-422. Genotype G3D2-413 may have a higher Fe content that still needs further confirmation.

Studies of the photosynthetic activity of perennial grass mixtures allow for describing the peculiarities of the formation of perennial grass species forage productivity in the dry steppe zone. The study aimed to evaluate the cumulative effect of perennial cereal and legume grasses as part of complex grass mixtures on photosynthetic activity, productivity, and nutritional value of mowing and grazing phytocenoses. Adapting multicomponent grass created from various species of perennial grasses to the continental climate of the steppe zone of Northern Kazakhstan commenced, capable of resisting biotic and abiotic stresses due to biological characteristics and positively influencing other species in the phytocenoses. The study determined photosynthetic activity, the yield of green and dry masses, dry matter content, crude protein, metabolizable energy of natural pasture, single-species sowing of awnless brome, and multicomponent grass mixtures. The communal herbage obtained as a result of the study contained a large amount of crude protein (20.6%–24.7%), crude fat (2.0%–4.2%), crude fiber (18.0%–22.7%), crude ash (7.1%–7.7%), and nitrogen-free extractive substances (35.0%–44.1%). The results further enunciated that the perennial grass herbage in question had an average photosynthetic potential of 1,450,330 m²×day ha-¹ in single-species awnless brome crops, while in grass mixtures the said potential was higher, ranging from 1,510,250 to 1,815,250 m²×day ha-¹. The authors concluded that it was necessary to create mowing and grazing fields composed of productive and stable perennial grass mixtures on degraded pasture lands to increase available forage for farm animals and improve the system’s resistance to adverse conditions.

Keywords: Perennial grasses and legumes, grass mixtures, pastures, hayfields, photosynthesis, green and dry yield, nutritional value, chemical composition

Key findings: Multicomponent grass-and-legume concoctions of the first and second year of life ensure the formation of higher yields, the nutritional value of forage, and photosynthetic potential (PP) compared with single-species crops. Multicomponent grass mixtures with the participation of drought-, salt-, and frost-hardy crops (alfalfa, sainfoin, brome, wheatgrass) have high ecological adaptivity, grow and overwinter well, and have high viability, which allows for the successful use in improving old degraded hayfields and pastures.

Soybean (Glycine max [L.] Merr.) is an economically important oilseed crop with an annual increase in growing grain demand. Soybean is a moderately salt-tolerant crop; however, salt stress conditions can affect its growth and yield-related traits and, eventually, reduce productivity. In saline soils, one of the techniques to increase soybean productivity is to use rhizobia inoculation. Although, using industrial rhizobia-based biofertilizers is often ineffective due to their lack of adaptability to salinity. Injecting soybeans with salt-tolerant and growth-promoting rhizobia helps mitigate the effects of salt stress harmful to crop plants. The recent study sought to isolate local strains of salt-tolerant rhizobia, studying its ability to increase soybean tolerance to salt stress conditions. Twenty-four local salt-tolerant rhizobium isolates underwent isolation from root nodules of soybean grown on saline soils. Studying their basic morphological and biochemical characteristics and ability to withstand salt stress led to the final selection of five salt-tolerant strains. The rhizobium strains were able to synthesize metabolites that stimulate growth and help reduce salt stress in plants. The study of rhizobia nodulation ability under saline conditions resulted in selecting the three most efficient strains from the Bradyrhizobium japonicum species. Inoculation of soybean seeds with salt-tolerant rhizobia proved to mitigate the adverse effects of salinity on plant growth by increasing the root size and the number of nodules in the roots. Thus, the study establishes that inoculation of soybean seeds with local salt-tolerant rhizobia enhances soybean tolerance to salt stress and improves crop growth and adaptation to soil salinity. Using isolated local strains of salt-tolerant rhizobia will help provide a key and environmentally friendly approach to solving the problem of salt stress for sustainable agriculture.

Keywords: Soybean (Glycine max [L.] Merr.), salt-tolerant rhizobia, inoculation, salt stress conditions, salt tolerance, growth and yield traits

Key findings: Soybean inoculation with salt-tolerant rhizobia significantly reduced the salt stress effects on the plants. Substantial differences also showed between the variants with inoculation and the control (untreated). Inoculating leads to a considerable increase in root nodules, nitrogen fixation, plant growth, and stimulation of root development, which is proof of the ecological adaptation of soybean plants to soil salinity.

The presented study, held at the Research cum Instructional Farm of the College of Horticulture and Research Station, Jagdalpur, Chhattisgarh, India, ran during the Rabi season of 2021, to investigate superior radish cultivars for selection having high yield potential and better quality root for Bastar plateau. The experiment, laid out in randomized block design (RBD), had 15 treatments and three replications. Observing high genotypic and phenotypic coefficient of variation was for the following traits: dry and fresh weights of roots, plants, and leaves; root and leaf yields; days to 50% germination; the diameter and length of the root, North-South and East-West spread of the plant; plant height, and the number of leaf plant^-1, having recorded significant heritability and high genetic advance. Similarly, high heritability showed for root yield and fresh and dry weights of roots. Moderate heritability levels appeared for the traits, viz., the plant’s dry weight and the root diameter. The genetic gain resulted high for characters, viz., root yield and fresh and dry weights of roots. High heritability followed by high genetic advance indicates that selection may be effective for improving such characteristics. Root yield (t ha^-1) exhibited a highly significant positive correlation with the fresh weight of the plant at the phenotypic and genotypic levels, followed by dry weight of roots, dry weight of the plant, East-West spread of the plant, the diameter of the root, and leaf yield plot^-1. The fresh weight of roots had the maximum positive direct effect on root yield in the genotypic path, followed by the dry weight of roots and the plant, the East-West spread of the plant, the diameter of the root, and leaf yield in radish. The selection of these characters can benefit in improving the yield of radishes.

Keywords: radish, path coefficient, root yield, genotypic, phenotypic and correlation

Key findings: The results indicated that a genetic variation of Raphanus sativus L. can further serve the species’ conservation, characterization, and usefulness for future breeding purposes. Cultivars exhibiting higher yield potential and desirable qualities may undergo testing in different agro-climatic locations.

Contemporary agriculture is a fertile ground for the effective use of economic and mathematical models, which can be evaluated to unwind several problems with characteristic optimization features: multiple solution opportunities and freedom of choice, limited production resources, and efficiency valuation. The presented study aims to develop a model of optimal crop production structure under the existing weather risks in the agricultural management system. The article reviews the basic theoretical concepts in optimizing the production structure of agricultural enterprises, examines the specific features of crop production, proves the influence of weather and climate conditions on forming the production structure, demonstrates the use of correlation and regression analysis for trend modeling and forecasting of crop yields, and offers suggestions for determining the optimal production structure. The study concludes that multivariate forecasting helps optimize management in economic organizations and ensure their development under the variability of natural conditions. Natural and climatic conditions significantly impact the development of production structures in agricultural enterprises, along with nature acting as an innate participant in the game.

Keywords: Agricultural enterprise, economic and mathematical models, natural conditions, agricultural production structure, optimization model, weather risks

Key findings: The proposed mathematical model helps maximize the net profit and predicts the maximum possible profit gain under different production structures depending on the existing weather conditions and risks. The presented model enables agricultural enterprise management to build business strategies based on profit maximization. The said model also provides a better justification of the managerial decision-making process for optimal planning to find the reserves to improve agricultural enterprises‘ production efficiency and marketing activities in times of crisis and dynamic changes in the external environment.

Assessment of botanical and genetic collection of soybean cultivars transpired during 2021–2022, for morphological and yield-related traits and their impact on nodule bacteria and soil fertility, at the Dormon Scientific Experimental Station, Institute of Genetics, Academy of Sciences of the Republic of Uzbekistan, District Kibray, Tashkent region, Uzbekistan. The soybean cultivars, grown in a randomized complete block design, had a factorial arrangement with three replications. Results revealed that in the botanical collection, the highest seed yield per plant came from the soybean cultivar K-2600 (48.3 ± 1.30 g), 1000-grain weight from Bk-105 (215.9 ± 0.15 g), and the number of grains per plant from the genotype K-2600 (346.4 ± 6.48). Moreover, in the genetic collection, the high oil content resulted in the genotypes Gen-15 (20.11%), Gen-13 (19.46%), and Gen-11 (19.40%). The higher values of the total protein content emerged in the soybean cultivars, Ehtiyozh (34.09%) and Gen-9 (33.74%), Gen-19 (34.72%), and Gen-26 (33.08%). Among the two types of soybean collections studied, the most substantial number of root nodules appeared in the cultivar Sochilmas (96.65 ± 0.11) during the pod formation phase. The recorded highest amount of soil microelements, according to soil analysis, was at the sowing site of the soybean cultivar, Genetiс-1 and on average, the soil samples contained N-NO₃ (28 mg/kg), mobile P₂О (212.3 mg/kg), and volatile trace element K₂О₅ (36 mg/kg), found to remain in the soil after crop harvesting. The conclusion based on the results indicates that the local soybean cultivar Genetik-1 has a more positive effect on improving soil fertility versus the other genotypes in both collections.

Keywords: Soybean (Glycine max L.), soil composition, macro-elements, botanical and genetic collections, morphology, yield attributes, oil and protein content, productivity

Key findings: Based on the presented findings, in the botanical collection, the highest grain yield per plant came from soybean cultivar K-2600, 1000-grain weight from Bk-105, and the highest number of grains per plant from the genotype K-2600. In the genetic collection, the highest oil content occurred in the genotypes Gen-15, Gen-13, and Gen-11. The highest total protein content emerged in the soybean cultivars Gen-19 (34.72%), Ehtiyozh (34.09%), Gen-9 (33.74%), and Gen-26 (33.08%). The cultivar Sochilmas collected the most nodule bacteria in the soil.

Developing high-yielding wheat varieties tolerant to abiotic and biotic stresses is a challenge wheat breeder currently face. Wheat Research Institute (WRI), Ayub Agricultural Research Institute (AARI), Faisalabad, Pakistan, has released 65 wheat varieties, including MH-21, for several zones since its establishment in 1906. MH-21, with the varietal code V-12304, gave 11.2% and 10.28% more grain yield than the check variety (Faisalabad-08), respectively, in preliminary (2011–2012) and regular yield trials (2012–2013). Then, testing it in outstation yield trials proceeded over 21 locations in Punjab province (2013–2014) and 18 locations all over Pakistan (2014–2015, 2017–2018, and 2019–2020) for yield and disease constancy, simultaneous with agronomic field trials and quality lab testing during 2014–2018 and 2014–2016, respectively. It exhibited an amber seed color, semi-erect growth habit at booting, erect flag leaf attitude, hairiness auricle, awned yellowish ear, and elevated shoulder and beak shape of glumes traits. DNA fingerprinting of MH-21 ensued to compare its genetic background with previously registered 21 wheat varieties. Given the promising stable performance in multi-locations over the years for better grain yield, nutritional quality, and resistance against rusts, the Punjab Seed Council approved V12304 in 2021 with the name ‘MH-21’ for general cultivation in irrigated areas of Punjab, Pakistan.

Keywords: MH-21, high yielding, rusts resistant, bread wheat irrigated areas, Punjab

Key findings: The approval of MH-21 gives fresh courage to redesign food security for the growers of irrigated areas overall Punjab, particularly in-explicit zones, by wheat yield maximization and tolerating the unpredicted disease threat.

Research carried out in 2021 at the Jeser Al-Musayyab Company, Baghdad, Iraq, determined the molecular variations among 10 inbred lines of maize (Zea mays L.), for use in the future production of promising F₁ hybrids. DNA sequencing random polymorphism technology using Inter Simple Sequence Repeats (ISSR) with five primers (UBC 834, UBC 835, UBC 856, UBC 866, and UBC 897) assessed the genetic variations among the 10 inbred lines of maize. All five primers produced 29 bands, averaging 5.8 strips each primer. The four primers, UBC 834, UBC 835, UBC 866, and UBC 897, had produced the same and highest number of proliferators with molecular weights ranging from 100 to 2000 bp. The primer UBC 856 showed the lowest number, with a molecular weight of 100–500 bp. The total number of polymorphs was 24, with a percentage of 82.4%, with the lowest (80%) polymorphs recorded in the primer UBC 856. The least discriminatory efficiency and ability values were 17.24% and 16.66%, respectively. Relying on binary data and genetic divergence using the UPGMA method to create a genetic kinship tree, the maize lines were separated into two groups, i.e., A, B, secondary, and sub-secondary groups. The highest genetic similarity (with the lowest genetic divergence) was estimated according to the Nei and Li coefficient of 0.23 between the two lines HS and S-10. The presented findings can provide further guidance and help analyze the genetic diversity among the maize populations.

Keywords: maize, ISSR, molecular, DNA, primer, cluster analysis

Key findings: The presented results authenticated the efficiency of ISSR markers in diagnosing genetic divergence among the inbred lines of maize (Zea mays L.).

The family Cactaceae comprises many genera, has taxonomic controversies at the species level, and yet has not been studied at the genetic level in Iraq. The presented study focused on species genotyping based on the RAPD-PCR method. The classification of six species, i.e., Hylocereus undatus, Aloe vera, Opuntia ficus-indica, Espostoa guentheri, Echinocactus grusonii, and Mammillaria elongata, ensued based on phenotypic characters to determine their precise taxonomic names and evaluate kinship by constructing the phylogeny tree RAPD-PCR. The short oligo primers showed the highest polymorphic bands (100%), with no monomorphic and basal bands correlated among these species. This highly polymorphic relationship indicated that each species has a superior identity and unique evolutionary trend. The oligo primers were considered productive by showing highly distinct and sharp bands, while others showed faint bands. This research confirmed the efficacy of RAPD primers in measuring polymorphism, comparing genotypes, and identifying Cacti species using specialized RAPD markers.

Keywords: Cactaceae, genotyping, RAPD-PCR, Opuntia, Mammillaria

Key findings: Using RAPD molecular markers, the study revealed a highly polymorphic relationship among the species of the family Cactaceae and has a superior identity and unique evolutionary trend.