MOHAMMED A.J. and A.S.A. AL-JANABI
SUMMARY
The existent research aimed to study the effects of irrigation with liquefied water, saline-well water, and foliar application of aspartic and ascorbic acids on the chemical properties of grafted orange seedlings in the years 2020–2021 at the Horticulture and Forestry Division, Najaf Agriculture Directorate, Iraq. The main plot was the irrigation water (liquefied and saline-well water). Meanwhile, foliar application of aspartic acid (0, 100, and 150 mg.L-1) and ascorbic acid (0, 4000 mg.L-1) served as the second and third factors in subplots. Compared with the saline-well water, regular liquefied water had a significant positive impact on the improvement of chemical traits, i.e., the liquefied water attained the highest rate of nitrogen content in leaves (2.600%) compared with the saline-well water (2.239%). Ascorbic acid (4000 mg.L-1) also had a significant effect on the leaf’s contents, providing the highest percentage of phosphorus (0.4060%) and reduced sodium (0.5277%) compared with the control in the leaves of orange seedlings. The saline-well water with no addition of ascorbic acid (control) provided the highest average content of the amino acid proline (132.2 μg.g-1 fresh weight) in the sweet orange leaves compared with the other treatments and their interactions. Sweet orange seedlings with foliar application of aspartic and ascorbic acids authenticated that the peroxidase activity rate appeared more effective than all other treatments and the control.
Keywords: Sweet orange transplants, liquefied water, saline-well water, aspartic and ascorbic acids
Key findings: Salinity impacts agriculture, thus the need to induce crop plants with salt tolerance to sustain their economic output. Antioxidants, such as, aspartic and ascorbic acids, have auxinic action and a synergistic effect on salinity tolerance and plant growth.
Date published: December 2022
DOI: http://doi.org/10.54910/sabrao2022.54.5.25
N. FATIMA, A. SAEED, A. SHAKEEL, and F.S. AWAN
SUMMARY
Salinity consists of critical abiotic stress adversely affecting tomato growth and development. Given the increase in saline areas, breeders endeavor to develop crops that can tolerate salinity. It indicates the importance of genotypes that can grow in salt-affected soil to cope with the problem. This study focused on identifying salt-tolerant and salt-sensitive genotypes using Principal Component Analysis (PCA). This study used a two-factor factorial under a complete randomized design, with three replications and three levels (T0 = control, T1 = 6 dS/m, T2 = 12 dS/m) of salt (NaCl) treatment. Data collection ensued at the seedling stage. Data for various morphological and biochemical attributes were recorded and subjected to analysis of variance and PCA to check the variation in germplasm and identification of suitable genotypes. Analysis of variance showed significant results for all attributes indicating the presence of variability in germplasm. Using PCA identified tolerant and non-tolerant tomato genotypes. Based on the results obtained from PCA analysis, genotypes AUT-318, CLN-2498A, 17884, Picendanto, 17260, 17256, 17263, and 17266 showed as salt tolerant, whereas the 19903, 19908, Target-66, H-24, 17255, Nadir, and Peelo displayed as salt-sensitive genotypes. Selected genotypes suit further use for the development of breeding material.
Keywords: Tomato germplasm, salinity, principal component analysis, osmolytes, screening, selection
Key findings: Based on results obtained from the analysis, the genotypes AUT-318, CLN-2498-A, 17884, Picendanto, 17260, 17256, 17263, and 17266 proved salt-tolerant. These selected genotypes suit future use for salt-tolerant varieties and hybrid development.
Date published: December 2022
DOI: http://doi.org/10.54910/sabrao2022.54.5.17
J.M. AL-KHAYRI, A.A.H. ABDEL LATEF, H.S.A. TAHA, A.S. ELDOMIATY, M.A. ABD-ELFATTAH, A.A. REZK, W.F. SHEHATA, M.I. ALMAGHASLA, T.A. SHALABY, M.N. SATTAR, M.F. AWAD, and A.A. HASSANIN
SUMMARY
Advancements in DNA sequencing technologies with decreasing costs have sparked the generation of larger gene expression datasets generated at an accelerating rate. The study aimed to visualize the spatiotemporal profiles of the tomato (Solanum lycopersicum L.) genes involved in L-proline biosynthesis and to show their potential functions. Increasing L-proline accumulation, through upregulation and downregulation of genes responsible for L-proline biosynthesis and degradation, plays an essential role in tomato plants suffering abiotic and biotic stress. Understanding the possible mechanism of L-proline biosynthesis and degradation needs an urgent study of the expression pattern and function of genes involved in these physiological processes. The study identified the genes governing the L-proline biosynthesis and degradation pathways and their expression profiles in 10 stages of tomato fruit development using the Tomato Expression Atlas (TEA) bioinformatic tool. The analysis showed that L-proline biosynthesis resulted from three pathways governed by six genes, while its degradation occurred in two pathways managed by three genes. The bioinformatics analysis also showed the expression of proline synthesis/degradation-related genes in fruit parts at various developmental stages. However, proline degradation-related genes showed higher expression levels than biosynthesis-related genes. This study sheds light on a recent bioinformatics tool, which will pave the way to detect early plant performance by analyzing the expression profiles of genes.
Date published: September 2022
Keywords: Tomato, bioinformatics, L-proline accumulation genes, gene expression, proline biosynthesis genes, salinity, drought
DOI: http://doi.org/10.54910/sabrao2022.54.3.8