Veronica is a prominent genus belonging to the family Plantaginaceae. Six species samples came from different mountainous areas of AL-Sulaimaniyah, Northern Iraq, comprising V. anagalloides, V. anagallis-aquatica, V. beccabunga, V. minuta, Veronica persica, and V. polita. DNA genomic manually extracted from plant leaves, and the specific PCR fragments partially covering the internal transcribed spacer-1, internal transcribed spacer-2, and 5.8S rRNA, and internal transcribed spacer-2 sequences were options for the latest study that proceeded with primers ITS5 (5′ GGAAGTAAAAGTCGTAACAAGG-3′) and ITS4 (5′-TCCTCCGCTTATTGATATGC-3′) to amplify the samples of six different Veronica species. The electrophoresis migration for PCR analysis showed that the length of the amplified segment was about 600 bp for all the studied samples. An assumption from the generated tree was that the detected nucleic acid variants showed a noticeable effect on changing the evolutionary positioning of the investigated samples compared with the wild-type sequences. All the investigated rRNA sequences deposited in the NCBI web server acquired unique accession numbers for the analyzed S1 to S3 sequences. The deposited sequences received the GenBank accession numbers, i.e., OP363796.1, OP363795.1, OP363794.1, OP363793.1, OP363792.1, and OP363791.1, representing the six investigated isolates of the Veronica species, respectively.
Veronica species, Plantaginaceae, ITS primers, DNA sequences, genetic variations
The variation of the rRNA sequences can also help in Veronica characterization due to its possible ability to adapt to variable genetic diversity. The sequencing reactions indicated the precise identity after performing NCBI BLASTn for these PCR amplicons. Concerning the investigated ribosomal amplicons, the NCBI BLASTn engine showed 99% to 100% sequence similarities between the sequenced samples and the intended reference target sequences.