The Aglaonema cultivar Silver Bay is distinct with its highest cost compared with other cultivars. In previous years, the necessity of research aimed at optimizing plant propagation processes has been increasing. The consequent study sought to conduct experiments on the optimization of the different stages involved in microclonal propagation of this important plant. Initially, clarifying the sterilization conditions of plant materials succeeded. Sterilization of materials in Domestos for 20 minutes, in 70% ethanol for two minutes, and in 0.1% AgNO₃ solution for three minutes allowed the increase in viability to 87.6%. Moreover, to enhance the efficiency of multiplication and rooting stages through microclonal propagation, standards for enriching the Murashige and Skoog nutrient medium with benzylaminopurine (BAP) and metatopolin were options. This tested combinations of substrates that increase the viability of regenerants at the rooting stage. Under selected optimal conditions, the number of shoots formed by explants was 2.6, reducing the emergence of shoots to 8.2 days, having root length at the fifth week of rooting at 6.7 cm, and the viability of seedlings during acclimatization reaching 89.4%. The obtained results could serve to increase the diversity of methods used in the propagation of indoor plants.

Keywords: Aglaonema, benzylaminopurine (BAP), cultivar Silver Bay, indole-3-butyric acid (IBA), in vitro, microclonal propagation, Murashige and Skoog, sterilization

Key findings: Research into optimizing the stages of microclonal propagation of Aglaonema has provided scientific solutions for increasing the efficiency of seedling production. The results obtained will serve to improve methods for mass propagation of ornamental plants.