E. RESIDA, T. CHIKMAWATI, N. ARIYANTI, and FITMAWATI
Citation: Resida E, Chikmawati T, Ariyanti N, Fitmawati (2023). Mangifera kemanga Blume (Anacardiaceae) taxonomic assessment for genetic diversity based on molecular substantiation. SABRAO J. Breed. Genet. 55(1): 175-186. http://doi.org/10.54910/sabrao2023.55.1.17.
Mangifera kemanga Blume is a wild relative of mango (Mangifera indica) and a local fruit with various potential uses. The community uses its fruit and leaves for food and its wood as a building material. However, the genetic diversity and taxonomic status of M. kemanga species still need more exploration and have a dispute with M. caesia Jack. The presented study aimed to determine the taxonomic status of M. kemanga and its relationship with M. caesia through a molecular approach and to recognize the species’ delimitation. The recorded data analysis used the trnL-trnF intergenic spacer sequence. Carrying out DNA isolation employed the Geneaid Genomic DNA Mini Kit (Plant) protocol, with the DNA sequences analyzed for kinship using Maximum Parsimony and Neighbor-Joining methods and genetic diversity analysis performed using DnaSP 6. A total of 27 trnL-trnF intergenic spacer sequence identification and authentication resulted from BLAST on NCBI as sequences derived from the genus Mangifera. The phylogenetic tree revealed that the accessions of M. kemanga and M. caesia are very similar, hence, considered not as independent species. Thus, based on the trnL-trnF intergenic spacer sequences, M. kemanga is a synonym of M. caesia with taxonomic status as a variety of M. caesia. This molecular-based taxonomic evidence is significant for determining the valid species status of M. kemanga so that it can provide basic information for further studies on biodiversity and germplasm conservation.
Keywords: Mangifera caesia, genetic diversity, molecular analysis, morphological traits, phylogenetic, trnL-trnF intergenic spacer marker
Key findings: Based on the accessions of M. kemanga and M. caesia collected from four islands in Indonesia, collecting molecular data used the trnL-trnF intergenic spacer marker, which provides several sequences that have yet to be published. The molecular analysis gave new taxonomic substantiation to determine the status of M. kemanga. Furthermore, the present data
SABRAO Journal of Breeding and Genetics
55 (1) 175-186, 2023
http://doi.org/10.54910/sabrao2023.55.1.17
http://sabraojournal.org/
pISSN 1029-7073; eISSN 2224-8978
Date published: February 2023
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