S. YURIYAH, L. HERLINA, H. AFZA, J. PRASETIYONO, A. DADANG, Y.N. ANDARINI, and A. MARUAPEY
Citation: Yuriyah S, Herlina L, Afza H, Prasetiyono J, Dadang A, Andarini YN, Maruapey A (2026). Development of stable and high-yielding rice (Oryza sativa L.) genotypes for suboptimal agroecosystems. SABRAO J. Breed. Genet. 58 (1) 12-22. http://doi.org/10.54910/sabrao2026.58.1.2.
Summary
Stable and high-yielding rice (Oryza sativa L.) cultivars are essential for improving productivity in marginal environments such as Papua, Indonesia. Ten rice genotypes, including elite and advanced breeding lines, underwent evaluation across two contrasting sites—Muara (favorable) and Papua (suboptimal)—to estimate genetic variability, heritability, stability, and tolerance indices. Significant genotype-by-environment interactions were evident for key agronomic traits, particularly plant height, productive tiller number, and 1000-grain weight. Moderate to high heritability (H² = 0.593–0.745) indicated a strong genetic control, with the productive tiller number showing the greatest variability (GCV = 14.68%, PCV = 23.80%). Stability analyses using the Eberhart-Russell and AMMI (additive main-effects and multiplicative interaction) models identified the genotype 12F as highly adaptable and high yielding, 1F and 54F as broadly stable, and 58F and 44F as consistently stable under stress-prone conditions. The stress tolerance index (STI) supported these findings, confirming combined yield potential and environmental resilience. Although based on two locations, this study provides valuable preliminary insights for developing rice cultivars adapted to suboptimal agroecosystems.
Significant genotype-by-environment interactions were noteworthy for primary agronomic traits, underscoring the importance of multi-environment assessment of rice (O. sativa L.). Stability analysis identified 12F as the most promising genotype, providing high yields and considerable adaptability.
R. LISTIYANTO, W.B. SUWARNO, Y. PULUNG, and H. ASWIDINNOOR
Citation: Listiyanto R, Suwarno WB, Pulung Y, Aswidinnoor H (2026). Rice breeding strategies for the multi-canopy system: Evidence from the evaluation of 37 two-genotype combinations. SABRAO J. Breed. Genet. 58 (1) 1-11. http://doi.org/10.54910/sabrao2026.58.1.1.
Summary
A multi-canopy cropping system—where short and tall rice (Oryza sativa L.) genotypes involve closer planting on the same hill—can considerably enhance the grain yield. Although the factors involved in this yield advantage have been the subject of intensive studies, the breeding of rice cultivars for such a cropping system remains at an early stage. The following study aimed to identify the effective breeding strategies for developing rice genotypes adapted to a multi-canopy system. Rice advanced lines totaling 35 from the F5 and F6 generations received evaluation under monoculture and multi-canopy systems. The field experiments, laid out in a randomized complete block design, had three replications in a multi-canopy and two in the monoculture. The multi-canopy system demonstrated more considerable sensitivity than monoculture in distinguishing genotypes’ performance and thus became identified as an ideal environment for selecting rice lines aimed for multi-canopy. For multi-canopy rice, the proposal for two primary selection strategies emerged, namely, sequential selection and simultaneous selection. For developing short genotypes, the key selection traits were plant height, the number of productive tillers, and filled grains per panicle. Under a multi-canopy system, the weighted index selection based on these traits proved to be effective in achieving the breeding objectives.
The multi-canopy system emerged to be effective in differentiating the rice (O. sativa L.) genotypes’ performance targeted for this system, and the weighted index selection was considerably reliable for selecting suitable rice genotypes.
Citation: Al-Najjar MAH, Alpresem WFF, Saleh AM (2025). Effect of polyamine compounds and zeolite on the anatomical traits of date palm offshoot leaves grown under heavy metal stress conditions. SABRAO J. Breed. Genet. 57(6): 2722-2730. http://doi.org/10.54910/sabrao2025.57.6.45.
Summary
Besides the control treatment, Jabjabb date palm (Phoenix dactylifera L.) offshoots received irrigation water contaminated with heavy metals, lead(II) nitrate (Pb[NO3]2) = 300 mg kg-1 and cadmium chloride (CdCl2) = 3 mmol. In addition to the control treatment, putrescine (500 mg L-1), coumaric acid (500 mg L-1), and 10 kg of palm-1 zeolite entailed application to seedling soil. After nine months of alternately treating the seedlings with pollutants and therapies, preparing tissue slices of the study palm leaves succeeded. Lead and cadmium treatment adversely affected leaf anatomy and decreased epidermal, parenchymal, and vascular bundle markers to the lowest values. The study showed putrescine, coumaric acid, and zeolite improved leaf tissue anatomical properties and recorded the highest values. The enhancers reduced pollution, but putrescine improved the anatomical properties of leaves impacted by heavy metals, most especially lead. This interaction had the highest epidermis thickness, parenchymal cell diameter, primary and secondary xylem diameter, phloem thickness, bundle sheath thickness, vascular bundle length, and width (145.53, 214.17, 90.88, 77.13, 62.12, 30.21, 36.11, and 11.00 micrometers, respectively). By modulating the thickness of leaf tissue cuticles, the heavy metal factor and enhancers boosted the plant’s pollution resistance.
Date palm (P. dactylifera L.), polyamines, zeolite, anatomical characteristics, palm offshoots, heavy elements, stress conditions
Heavy metal pollution negatively affected the anatomical characteristics and showed decreased values of epidermal cells, parenchymal cells, and vascular bundles in leaf tissue of the date palm (P. dactylifera L.). Bioenhancers, such as putrescine, coumaric acid, and zeolite, improved the anatomical properties of leaf tissues.
Citation: Ahmed AN (2025). Fusarium dimerum effect on chlorophyll content and morphological and anatomical traits of date palm. SABRAO J. Breed. Genet. 57(6): 2713-2721. http://doi.org/10.54910/sabrao2025.57.6.44.
Summary
This experiment transpired to determine the effect of the fungus Fusarium dimerum on the chlorophyll content and some phenotypic and anatomical morphological traits of the leaves of date palms (Phoenix dactylifera L.). The study results showed the ability of the fungus F. dimerum to cause infection on date palm leaves of five cultivars. The findings revealed that F. dimerum can secrete the enzymes cellulase, phenol oxidase, lipase, and amylases, as the area of their enzyme activity reached 6.1, 5.8, 6.2, and 12.4 mm, respectively. The results of histological analysis of the infected leaves also indicated the presence of F. dimerum spores in tissues of the infected leaves and the decomposition of cell walls and the loss of their integrated shape. The experiment showed significant differences in the chlorophyll content in healthy leaves and those infected with the fungus F. dimerum, wherein the percentage decreased in leaves infected with the fungus compared with the control treatment (healthy leaves).
Date palm (P. dactylifera L.), Fusarium dimerum, histological anatomy, chlorophyll content in leaves, enzymes, fungal disease
The results of the pathogenicity test for the fungus F. dimerum on the leaves of five cultivars of date palms (P. dactylifera L.) showed the ability of the fungus F. dimerum to cause infection and its ability to secrete the enzymes cellulase, phenol oxidase, lipase, and amylases. The ability to secrete enzymes plays a primary role in causing infection in the date palms.
Citation: Al-Doori SAMA (2025). Humic acid and plant density effect on growth, yield components, and quality of safflower (Carthamus tinctorius L.). SABRAO J. Breed. Genet. 57(6): 2701-2712. http://doi.org/10.54910/sabrao2025.57.6.43.
Summary
In determining the best level of humic acid foliar application and suitable plant density and their effect on growth traits, yield components, and the quality of three safflower (C. tinctorius L.) cultivars, an experiment commenced during the winter crop season of 2019–2020 at two locations. The first location was Zmmaar Village, Mosul City, and the second location was in the Sallamiya Region, south of Mosul City, Iraq. The experiments had a randomized complete block design (RCBD) layout with factorial arrangement and three replications. Each experiment included three levels of humic acid (HA1: 0, HA2: 4, and HA3: 8 mg L-1) with three plant densities (PD1: 46,296, PD2: 69,444, and PD3: 138,888 plants ha-1) and three safflower cultivars (C1: Goldasht, C2: Oleic Leed, and C3: Aswan). The safflower with a humic acid concentration of 8 mg L-1 resulted in a significant increase in growth, yield, and quality traits compared with the lowest concentration (4 mg L-1) and the control treatment in both locations. Plant density (46296 plants ha-1) was superior by giving the highest seed yield per plant, biological yield, harvest index, oleic and linoleic acid, and oil and protein percentages for both locations. The safflower cultivar Aswan achieved the highest rate of growth, productivity, and quality traits compared with cultivars Oleic Leed and Goldasht at both locations.
Safflower (C. tinctorius L.) cultivars, humic acid, plant density, seed yield, biological yield, harvest index, oleic and linoleic acid, oil and protein percentage
The safflower (C. tinctorius L.) cultivars with humic acid foliar application (8 ml L-1) resulted in a significant increase in growth, yield, and quality traits compared with the lowest concentration (4 ml L-1) and the control treatment in both locations.
Citation: Al-Doori SAMA (2025). Response of rapeseed genotypes to different tillage systems and foliar application of humic acid. SABRAO J. Breed. Genet. 57(6): 2691-2700. http://doi.org/10.54910/sabrao2025.57.6.42.
Summary
This study sought to measure the yield and quality of rapeseed (Brassica napus L.) and their response to different tillage systems and foliar spraying with humic acid within the conditions of Mosul City. A field experiment proceeded during the winters of 2020–2021 and 2021–2022 in the Al-Rashidia Region, Iraq (longitude 43.15° East and latitude 36.35° North), to study the response of three rapeseed genotypes (G1: Lirakotta, G2: Edita, and G3: Diamant) to three tillage levels. The first was conventional (CT: conventional tillage), which plowed the land with a perpendicular disc plow before field division; the second level was minimal tillage (MT: minimal tillage), leveling the soil and reducing tillage; and the third level was no-tillage (NT: no-till). Additionally, applying foliar spraying with three concentrations of humic acid (0, 5, and 10 mg L-1) emerged at the six-leaf stage. The experiment, carried out using a factorial experiment in a randomized complete block design, had three replicates for each experiment. The following results were notable. The Diamant genotype gave the highest mean for all growth, yield, and quality traits for both growing seasons. Tillage systems significantly affected all categories of growth, yield, and quality traits in two planting seasons (2020–2021 and 2021–2022). Foliar application of humic acid at the concentration of 5 mg L-1 gave a significant increase in all growth, yield, and quality traits for both growing seasons.
The rapeseed (B. napus L.) genotype Diamant gave the highest mean for all growth, yield, and quality traits for both growing seasons. Tillage systems had a significant effect on all categories for growth, yield, and quality traits in two planting seasons of 2020–2021 and 2021–2022.
Citation: Al-Miahy FHR, AL-Jaf IHM (2025). Molecular study of the olive (Olea europaea L.) grown in Central and Southern Iraq. SABRAO J. Breed. Genet. 57(6): 2684-2690. http://doi.org/10.54910/sabrao2025.57.6.41.
Summary
The research dealt with olive (Olea europaea L.) cultivars cultivated in the central and southern regions of Iraq by using the ISSR technique. It aimed to determine the degree of relatedness and draw the phylogenetic tree of the studied cultivars using nine primers. The study also used ISSR indicators in recognizing the relationships among the cultivars. The primers used gave 68 bands, showing the 56 formed had multiformats with an average of 82% for the multi-banding format. Moreover, the primers used succeeded in giving polymorphism among the resulting bandings, as the highest percentage of polymorphism reached 90% in the primers CT7CG, while the primer AG10G did not give more than 71.3% of polymorphism. It indicated that the highest percentage of similarity corresponding to the least genetic dimension (0.80) was between the two types—Shami and Qaisi, and Shami and Satakatrina—which were the most distant genotypes from the rest of the varieties. In conclusion, the study successfully utilized the ISSR technique to assess the genetic relationships among olive cultivars in central and southern Iraq. Hence, it provides useful information in understanding the genetic structure of olive cultivars in the region and could benefit as a guide for future breeding programs and the conservation of genetic resources programs.
The ISSR technology effectively distinguished olive (O. europaea L.) genotypes and revealed their genetic relationship and the degree of genetic kinship between the genotypes.
Citation: Abdhussian HM, Sarheed AF, Musa AKJ (2025). Genetic and phenotypic correlations in yield-related traits of rice hybrids using factorial hybridization. SABRAO J. Breed. Genet. 57(6): 2676-2683. http://doi.org/10.54910/sabrao2025.57.6.40.
Summary
The particular research aimed to evaluate the performance of 24 rice (Oryza sativa L.) genotypes comprising four local (Amber 33, Yasmine , Al-furat , and dajla) and four Egyptian cultivars (Giza 201, Giza 178, Giza 179, and Basmati 1). Completing the 24 were 16 hybrids obtained through the factorial hybridization method held in 2021 and 2022 at the Rice Research Station, Al-Mishkhab, Al-Najaf Al-Ashraf, Iraq. Significant differences were evident among the genotypes (varieties and their hybrids) in all studied traits. The hybrid Giza 201 × Al-furat excelled on traits of 1000-grain weight, biological yield, grain yield per plant, and harvest index (27.60 g, 429.33 g, 234.67 g, and 54.66%, respectively). The hybrid Basmati 1 × Dajla also excelled for biological yield, grain yield, and harvest index (405.33 g, 196.67 g, and 48.55%, respectively). Similarly, the cultivar Al-furat stood out for the above traits (367.67 g, 192.67 g, and 52.41%, respectively).
The rice (O. sativa L.) hybrid Giza 201 × Al-furat excelled for the traits of 1000-grain weight, biological yield, grain yield per plant, and harvest index (27.60 g, 429.33 g, 234.67 g, and 54.66%, respectively).
This study on hawk moths, belonging to the family Sphingidae found in different regions of Uzbekistan, was successful in its conduct during 1998–2024, as well as research of literature sources. In total, the work recorded 27 species that belong to 16 different genera in the republic. The study sample collection came from the different regions, as follows: 791 from the northwestern regions, 443 from the central and southern regions, and 342 from the eastern regions in the Fergana Valley. Analysis based on varied landscapes revealed the collection of 563 samples (35%) originated from deserts, 441 samples (27%) from mountainous regions, and 628 samples (38%) procured from the lowlands and agro-landscapes. Most samples collected took place during spring, summer, and autumn. Twenty-one out of 27 hawk moth species recorded emerged during the research, while the remaining six species identified were reliant on the literature sources and material obtained from the Zoology Institute, Academy of Sciences, Uzbekistan.
Sphingidae, hawk moths, biogeography, ecology, distribution, Northwestern and Central Uzbekistan
The promising faunal analysis revealed hawk moth species appeared widely distributed in three regions of Uzbekistan. The species composition also varies across the different regions.
S. NIZAMOV, N. ABDURAKHMONOV, J. KUZIEV, A. KORAEV, M. MIRSODIKOV, N. KALANDAROV, KH. NURIDDINOVA, N. XALILOVA, and Z. BAKHODIROV
Citation: Nizamov S, Abdurakhmonov N, Kuziev J, Koraev A, Mirsodikov M, Kalandarov N, Nuriddinova KH, Xalilova N, Bakhodirov Z (2025). Ecological state of irrigated soils and ways to improve it. SABRAO J. Breed. Genet. 57(6): 2659-2667. http://doi.org/10.54910/sabrao2025.57.6.38.
Summary
In the Chirchik oasis and Almalyk region of Uzbekistan, industrial activities and intensive agriculture have led to considerable contamination of irrigated soils with toxic elements. The presented study aimed to evaluate the agrochemical state of irrigated serozem and irrigated meadow soils under the influence of the JSC Maksam-Chirchik and the Almalyk Mining and Metallurgical Complex, respectively. For restoring soil fertility, a biological remediation technology including plowed-in tree leaf litter, inoculation with actinomycete strains, and introduction of earthworms took place through lysimetric experiment. Laboratory analyses over spring and autumn showed remediation treatment increased soil nutrient availability. The nitrate nitrogen rose from 16.0 mg/kg (spring) to 22.0 mg/kg (autumn), and phosphorus also increased in treated soils. The mobile potassium level decreased by autumn, likely due to crop uptake and leaching. The contaminated soils contained aluminum (up to 72.4 mg/kg) and water-soluble fluorine (up to 64.3 mg/kg) in spring, exceeding permissible limits; however, these toxic elements declined by autumn after remediation. Heavy metals (chromium and nickel) with trace amounts manifested in spring and declined to safe thresholds in autumn in polluted sites. The biological remediation technology significantly improved the soil’s health by enhancing macronutrient content, promoting humus formation, and reducing the concentrations of toxic elements in the soil. The results demonstrate the technology’s effectiveness in rehabilitating polluted irrigated soils and improving their ecological state.
The contaminated irrigated soils showed significant seasonal dynamics in nutrient content, with biological remediation increasing available nitrogen and phosphorus. The remediation technology effectively reduced the exchangeable aluminum and fluorine levels and accumulation of chromium, nickel, and persistent pesticides and improved soil fertility, demonstrating its potential for ecological restoration of irrigated soils.