Q.S. AL-NEMA and R.M. ABDULLAH
Citation: Al-Nema QS, Abdullah RM (2023). Propagation protocol of the medicinal plant – aloe vera using tissue culture. SABRAO J. Breed. Genet. 55(1): 254-259. http://doi.org/10.54910/sabrao2023.55.1.23.
Aloe vera is one of the most popular cactus-type plants in the global market due to its widespread uses in pharmaceutical, cosmetic, food, and decorative purposes. The present study derived callus cultures from the Aloe vera plant leaves, then reproduced on agar-solidified MS medium from June to December 2021 at the University of Mosul, Iraq. Results revealed that the MS medium + 3.0 mg L-1 benzyl adenine (BA) proved suitable for induction of leaf callus up to 85%, while the MS medium supplement with 1.0 and 2.0 mg L-1 BA reached 70%. The MS medium with 1.0 mg L-1 BA showed the best results for growing apical shoots of A. vera plants and producing vegetative branches. The formation of roots emerged within two weeks after placing them on the rooting medium. The shoots regenerated from the growing apices and were rooted easily in agar-solidified MS medium. The obtained plants attained successful acclimatization in terms of their growth and length, afterward, transferred to the peat-moss mixture.
Keywords: Aloe vera, callus cultures, propagation, leaves, stems, apical shoot
Key finding: The study aimed to identify the behavior of Aloe vera plants in the culture medium represented by the formation of callus cultures and their differentiation.
SABRAO Journal of Breeding and Genetics
55 (1) 254-259, 2023
http://doi.org/10.54910/sabrao2023.55.1.23
http://sabraojournal.org/
pISSN 1029-7073; eISSN 2224-8978
Date published: February 2023
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