The article presents data on the study of in vitro growth efficiency of regenerated plants and tuber yield of minitubers of potato (Solanum tuberosum L.) cultivars under greenhouse conditions during 2022–2024. The shoot biomass growth and productivity of minitubers depended on the temperatures and biological parameters of potato genotypes. According to the results, the recorded maximum tuber yield formation appeared in cultivars Babayev, Tamasha, and Zhanaisan (84.6, 67.21, and 65.66 g/plant). The cultivar Babayev showed the best tuber yield, averaging over three years (72.5 g per plant and 1087.5 g/m²). Moreover, the cultivar Babayev displayed the highest multiplication rate in the experiment (6.0 minitubers per plant in 2024 and 5.4 pieces on average for three years). The said promising cultivar can be effective as a donor parent for tubers in future breeding. Similarly, the bioorganic fertilizers’ effect succeeded in determining the growth and development of virus-free test tube plants and the formation of potato minitubers. The findings revealed bioorganic fertilizers have a positive effect on the productivity of potato minitubers grown in the greenhouse. The biohumus in combination with liquid humic fertilizer increased the multiplication factor of the source material and its tuber yield in the primary seed production.

Keywords: Potato (S. tuberosum L.), cultivar, seed production, minitubers, biotechnology, greenhouse, bioorganic fertilizers, growth, productivity

Key findings: A series of experiments continued to ensure the maximum tuber yield of minitubers per unit area of potato (S. tuberosum L.) on protected soil. The organic fertilizers also revealed a positive effect on the growth and development of virus-free test tube plants and the formation of potato minitubers.