MOLECULAR IDENTIFICATION OF THE POTATO VIRUS M ISOLATE PVM-UZ AY1 WITH COAT PROTEIN (CP) GENE AND PHYLOGENETIC ANALYSIS

MOLECULAR IDENTIFICATION OF THE POTATO VIRUS M ISOLATE PVM-UZ AY1 WITH COAT PROTEIN (CP) GENE AND PHYLOGENETIC ANALYSIS

A.A. YUSUBAKHMEDOV, V.B. FAYZIEV, B. ADILOV, T.KH. MAKHMUDOV, B.A. ABDUVALIEV, S.K. KURGANOV, Е.Е. BEKMATOVA, A.A. TEMIROV, SH.P. ERDANAEVA, and D.T. ATABAEVA

Citation: Yusubakhmedov AA, Fayziev VB, Adilov B, Makhmudov TKH, Abduvaliev BA, Kurganov SK, Bekmatova EE, Temirov AA, Erdanaeva SHP, Atabaeva DT (2024). Molecular identification of the potato virus M isolate PVM-UZ ay1 with coat protein (CP) gene and phylogenetic analysis. SABRAO J. Breed. Genet. 56(5): 1769-1777. http://doi.org/10.54910/sabrao2024.56.5.2.

Summary

Potato virus M (PVM) is one of the most widespread and economically damaging viruses worldwide, along with X, Y, S, A, and L. Therefore, outcomes of the monitoring surveys carried out in potato fields during this study showed numerous disease symptoms visible on potato plants, such as boat-shaped curling of leaf tips, mosaic twisting, leaf swelling, striped mosaic along the veins, and spotted mosaic. The diseased sample collections from potato plants and the molecular and genetic identification progressed based on the PVM protein coat (CP) gene. The results revealed that the boat-shaped curling and mosaic symptoms were PVM’s typical signs. The isolates’ identification continued through PCR product sequencing, and their submission in the NCBI database received a PP235793.1 tag and the name PVM-Uz-AY1. The phylogenetic analysis of the identified isolates showed that the said isolate was 98% related to the isolate GQ469609.1, and the rest to isolates, such as VF-Nevsky2 (LC511899.1), FE-Queen_anne10 (LC511893.1), and KC479342.1. Those located on other phylogenetic branches were KF561611.1, KF561603.1, and KF561608.1, which appeared to share 99% homology. In addition, the study identified the virus using the Real Time-PCR method, obtaining reliable results.

Potato (Solanum tuberosum L.), PVM, isolate, RT-PCR, CP gene, PVM-Uz-AY1, primer, phylogenic analysis, Uzbekistan

PVM-Uz-AY1 isolate of the PVM infecting potato (Solanum tuberosum L.) succeeded in identification using RT-PCR. The nucleotide sequence study of the CP gene responsible for this virus’ protein coat was intensive. The construction of a phylogenetic tree of the PVM-Uz-AY1 isolate occurred based on the bioinformatic analysis, determining the evolutionary origin of the virus.

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SABRAO Journal of Breeding and Genetics
56 (5) 1769-1777, 2024
http://doi.org/10.54910/sabrao2024.56.5.2
http://sabraojournal.org/
pISSN 1029-7073; eISSN 2224-8978

Date published: October 2024

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