Wheat (Triticum aestivum L.) is the most widely cultivated cereal worldwide, a staple food for 40% of the world’s population that contributes 20% of total dietary requirements. Yellow dwarf viruses are viruses affecting the grain yield and its quality and damaging the cereal crops economically. Yellow dwarf viruses are the most economically important plant viruses impacting cereal production worldwide, which include viruses from the genus Luteovirus BYDV-PAV, MAV, and Polerovirus CYDV-RPV. The Cereal Yellow Dwarf Virus (CYDV-RPV) is one of the most dangerous YDV viral species. Barley/cereal yellow dwarf viruses cause yellow dwarf disease, which is a continuous risk to cereal crop production globally. These viruses cause leaf yellowing and stunting, resulting in yield reduction of up to 80%. They are the most critical viral diseases of cereals worldwide. They have a wide host range, which includes wheat, barley, oats, and over 150 grass species. These viral infections have the potential to spread epidemics in Uzbekistan’s wheat fields during specific seasons and play a crucial role in the epidemiological process. Using the KibrayT1 isolate of the CYDV-RPV strain, the following study aimed to determine the prevalence of CYDV-RPV in wheat fields in the District of Kibray, Tashkent Region, Uzbekistan. The use of reverse transcription-polymerase chain reaction succeeded in making the diagnosis.
Wheat (T. aestivum L.), cereal yellow dwarf virus (CYDV), RT-PCR, CP gene, phylogenetic analysis, KibrayT1
The CYDV-RPV isolate detection was successful by RT-PCR in wheat (T. aestivum L.). The determination of the nucleotide sequence of the coat protein gene prevailed in this isolate. The phylogenetic analysis revealed its close relatedness to the Jordan CYDV-RPV isolate ‘Jordon’ (HQ206716.1).