Plant breeding’s rewarding polyploidy analysis can happen by observing molecular characteristics. Previously obtained 21 sugarcane mutants had used colchicine induction on the GMP3 cultivar at the PT Gunung Madu Plantations, Lampung, Indonesia. However, little information emerged related to the informative molecular markers and analysis of molecular characteristics in sugarcane mutants. The presented research complements previous studies regarding the characterization of superior sugarcane mutants based on agronomic and anatomical attributes. The said research ran through PCR-RAPD amplification using five primers. The DNA band pattern diversity analysis ensued using MVSP program version 3.2. The cluster analysis through UPGMA also estimated the genetic distance. Polymorphic band assessment employing the polymorphism information content (PIC) also helps determine the informative level of the used primers. The PCR-RAPD markers’ screening results showed that four informative primers (OPN-07, OPB-19, OPA-04, and OPA-07) have PIC values ranging from 0.30 to 0.39. The molecular characterization showed that 21 GMP3 mutants had 35–60 DNA bands with 28 polymorphic bands and a similarity index of 0.47–1.00. The promising results would help increase sugar production and improve quality by inducing mutation in the sugarcane cultivar GMP3.
Sugarcane (Saccharum officinarum L.), sugarcane cultivar GMP3, cluster analysis, genetic distance, markers, molecular analysis, PCR-RAPD, polymorphism information content (PIC)
In analyzing the molecular traits in colchicine-induced sugarcane mutants, the PCRRAPD markers OPN-07, OPB-19, OPA-04, and OPA-07 appeared relatively informative, with a PIC value of 0.30–0.39. The genetic variation analysis of 21 GMP3 mutants had 35–60 DNA bands with 28 polymorphic bands. The phylogenetic analysis showed that all sugarcane mutants have a similarity index value ranging from 0.47 to 1.00, with groupings based on mutagen concentration and soaking time.