The Barley Yellow Dwarf Virus (BYDV) is one of the most economically significant viruses from the Luteoviridae family that infects most crop plants of the family Poaceae. The presented study sought to molecularly identify BYDV in proso millet (Panicum miliaceum L.) and evaluate the genetic diversity of its CP (coat protein) gene concerning other strains worldwide. Plant samples collected from proso millet fields in the Navoiy Region, Uzbekistan, incurred BYDV-PAV RT-PCR for the CP gene. The results authenticated the presence of a BYDV-PAV strain in the collected samples. The identified nucleotide sequence of BYDV-PAV isolate T-UZB2 proceeded to deposit in the GenBank database. BLASTN analysis of this sequence showed the highest genetic similarity (98.75%) with the Pakistan PAV-type isolate PK3 (JX473288.1). Phylogenic analysis and multiple sequence alignment revealed that four mutation events (268G>A, 440A>C, 450A>C, and 464C>T) involving the BYDV-PAV isolate Pk3 Pakistan have considerably contributed to the evolutionary history of PAV T-UZB2 Uzbekistan haplotype. SMV identification can help control pathogens and reduce their impact on the soybean crop in Uzbekistan.
Proso millet (Panicum miliaceum L.) survey, barley yellow dwarf virus (BYDV), RT-PCR, CP gene, phylogenic analysis, Navoiy Region, Uzbekistan
The BYDV-PAV isolate detection in Uzbekistan occurred by RT-PCR in proso millet (Panicum miliaceum L.). The nucleotide sequence of the coat protein gene of this isolate reached recognition. Phylogenic analysis revealed its close relationship with the Pakistan PAV-type isolate PK3 (JX473288.1).