The process of selecting rice (Oryza sativa L.) tolerant to low-temperature stress through phenotype selection activities is a time-consuming process. However, in rice (Oryza sativa L.) genotypes, the genetic diversity can be assessed rapidly using molecular marker-based characterization. This study aimed to evaluate the genetic diversity and cold tolerance of 15 F4 rice lines derived from crosses and their six parental genotypes using five SSR markers. The genetic diversity analysis employed the NTSYS pc 2.1 software. Results showed 21 rice genotypes had allele sizes of 150 to 230 bp, with an average number of alleles (5.4) and a polymorphism level of 0.79. The genetic similarity coefficient level of 0.37 contained three clusters. Four promising rice lines (F4UKIT102R-2-100, F4UKIT102-2-024, F4UKIT102R-2-078, and F4UKIT102R-2-018) were classified as tolerant to low temperature and exhibited the best agronomic performance. The tolerant line F4UKIT102R-2-100 was notably very similar to the parental genotype Pare Bau. The three other lines, F4UKIT102-2-024, F4UKIT102R-2-078, and F4UKIT102R-2-018 were similar to the parental genotypes Pare Lallodo, Pare Kombong, and Pare Ambo, respectively.
Cold-tolerant rice (O. sativa L.) lines, molecular characterization, genetic similarity, new rice type
The category as tolerant to low temperature, along with the best agronomic performance, was achieved by four promising rice (O. sativa L.) lines (F4UKIT102R-2-100, F4UKIT102-2-024, F4UKIT102R-2-078, and F4UKIT102R-2-018))