Microclonal propagation outcomes of various cherry (Prunus avium L.) cultivars and rootstocks achieved their evaluation and establishment of combinations of optimal nutrient media and growth regulators for each genotype. The rootstock Krimskiy-5 showed the best response with 2.95 shoots and 1.84 cm length using the Driver and Kuniyuki Walnut (DKW) medium supplemented with 0.01 and 0.75 mg/l BAP (benzylaminopurine). Cherry rootstocks Gisela-5 and Gisela-6 produced 2.77 and 2.99 shoots, respectively, with similar conditions. In rootstock Colt, the use of 0.02 and 1.0 mg/l BAP resulted in 2.92 shoots with a 1.78-cm length. The cherry cultivars Bahor, Volove Serdse, and Revershon with mT (meta-Topolin) combinations in DKW medium provided shoot numbers above 2.9 and lengths over 2.1 cm. The cherry cultivar Kara Geles showed better performance with naphthaleneacetic acid (NAA) and Thydizoronate (thidiazuron or TDZ), achieving 3.22 shoots. The cultivar Pink Napoleon exhibited the highest proliferation, with 3.42 shoots and 3.52-cm shoot length. Overall, the DKW nutrient medium combined with specific growth regulators demonstrated superior performance in shoot proliferation across most cultivars, whereas Murashige and Skoog (MS) and Woody Plant Medium (WPM) media were less efficient. These findings contribute to the development of improved protocols for the micropropagation of cherry species.
Cherry (P. avium L.), auxins (IBA, NAA), cytokinins (BAP, mT, TDZ), nutrient medium, shoot, microclonal propagation
The highest micropropagation results resulted in the cherry (P. avium L.) cultivar Pink Napoleon using 0.02 mg/l NAA and 1.50 mg/l TDZ in DKW medium, producing 3.42 shoots and 3.52 cm length. This highlights the considerable synergistic effect of NAA and TDZ and the superior efficiency of DKW medium compared with MS and WPM.