GENETIC ANALYSIS AND EXPRESSION PROFILING OF TAHSP90A TRANSCRIPTS CONFER HEAT TOLERANCE IN WHEAT

GENETIC ANALYSIS AND EXPRESSION PROFILING OF TAHSP90A TRANSCRIPTS CONFER HEAT TOLERANCE IN WHEAT

A. AMMAR, Z. ALI, M.A.B. SADDIQUE, M. HABIB-UR-RAHMAN, and I. ALI

Citation: Ammar A, Ali Z, Saddique MAB, Habib-Ur-Rahman M, Ali I (2023). Genetic analysis and expression profiling of TaHSP90A transcripts confer heat tolerance in wheat. SABRAO J. Breed. Genet. 55(3): 653-670. http://doi.org/10.54910/sabrao2023.55.3.5.

Summary

Heat stress has emerged as a chief problem impeding wheat crop productivity. In several crops, specific HSP90A genes have intensively managed induced fluctuations in temperature. A wheat plant with TaHSP90A transcripts had the potential to cope with temperature stress. It enables plants to survive in transient extremes of temperature and under heat stress. The presented study design enhanced temperature tolerance plasticity with high yield in wheat through a line × tester mating design containing lines (12) and testers (4) having a differential expression of TaHSP90A transcripts (TraesCS2A02G033700.1, TraesCS5B02G258900.3, and TraesCS5D02G268000.2), then hybridized to get the F1 (48) wheat hybrids. For heat treatment, temperature raising was only in the daytime, through the tunnel at anthesis (for two weeks). Data recording for several morphological and physiological parameters went along with the relative expression of TaHSP90A transcripts for hybrid evaluation. After one hour of heat treatment, the relative expression of TaHSP90A transcripts’ determination in the flag leaf followed. The manifestation of TaHSP90A transcripts’ upregulation was two folds in several hybrids after heat treatment. Best lines, testers, and selected crosses having TaHSP90A transcripts with high yield and heat tolerance compared with parents can further benefit breeding programs aiming toward tolerance against heat stress in changing climate scenarios.

Triticum aestivum L., climate change, expression, TaHSP90A, food security

This study mainly focuses on the upregulation of TaHSP90A transcripts showing a contribution to heat tolerance in wheat. Heat-tolerant wheat genotypes with TaHSP90A transcripts can produce significant yields under changing climate scenarios. The importance of this work lies in the potential for these genes by breeders to improve the plant’s natural defenses against heat stress.

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SABRAO Journal of Breeding and Genetics
55 (3) 653-670, 2023
http://doi.org/10.54910/sabrao2023.55.3.5
http://sabraojournal.org/
pISSN 1029-7073; eISSN 2224-8978

Date published: June 2023

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