EVALUATION OF DOUBLED HAPLOID RICE LINES DERIVED FROM ANTHER CULTURE OF INPARI-42 GSR AGRITAN MUTANT IN ADVANCED YIELD TRIALS

EVALUATION OF DOUBLED HAPLOID RICE LINES DERIVED FROM ANTHER CULTURE OF INPARI-42 GSR AGRITAN MUTANT IN ADVANCED YIELD TRIALS

B.S. PURWOKO, A.P. NADIA, I.S. DEWI, R. HERAWATI, and M.F. ANSHORI

Citation: Purwoko BS, Nadia AP, Dewi IS, Herawati R, Anshori MF (2025). Evaluation of doubled haploid rice lines derived from anther culture of Inpari-42 GSR Agritan mutant in advanced yield trials. SABRAO J. Breed. Genet. 57(3): 1030-1040. http://doi.org/10.54910/sabrao2025.57.3.15.

Summary

Combining mutation and anther culture technology could accelerate the rice breeding program. This research evaluated the agronomic performance of doubled haploid (DH) lines derived from anther culture of Inpari 42 GSR Agritan mutant in advanced yield trials. The research was conducted in Bogor and Indramayu, Indonesia from December 2023 to April 2024. The material used was 18 DH lines and two check varieties (Inpari 42 GSR Agritan and Inpari 45 Agritan). The experiment used a randomized complete block design with three replications and the rice genotype as a factor. The result indicated a variation in agronomic characters, yield components, and productivity of the DH rice lines. The interaction of genotype by location was notable in the vegetative plant height, the number of vegetative tillers, the percentage of filled and empty grains, the weight of 1000 grains, and productivity. Highly significant and positive correlations to productivity were evident in total grain, percentage of filled grains, the number of filled grains, grain filling period, and days of harvesting. Selection used the index selection method based on productivity, percentage of filled grains, total grains, and days of harvesting, which indicated the 12 DH lines need further evaluation in multi-location trials for their potential yield and stability.

anther culture, correlation, green super rice, mutation, selection index, yield

The combined mutation and anther culture technology, followed by the index selection method, can accelerate in obtaining advanced rice lines with good agronomic and productivity characteristics.

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SABRAO Journal of Breeding and Genetics
57 (3) 1030-1040, 2025
http://doi.org/10.54910/sabrao2025.57.3.15
http://sabraojournal.org/
pISSN 1029-7073; eISSN 2224-8978

Date published: June 2025

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