Rodent tuber (Typhonium flagelliforme Lodd.) has anticancer bioactive compounds, including beta-sitosterol, stigmasterol, octadecanoic, and hexadecenoic acids. Its mutant plants showed a higher cytotoxic effect on breast cancer cells than its wild-type plants. In rodent tuber mutants, the genebased molecular markers associated with anticancer compounds have not reached identification. Designing the primer pairs at SNP sites observed in rodent tuber mutant lectin genes led to the latest study developing SNAP molecular markers. The exon part region of previous lectin gene sequences at 500 bp long also underwent analysis. In such a sequence, a three-point mutation helped analyze the amino acid transformation. A successful design of a pair of primers based on non-synonymous SNP sites was specifically for SNP sites that cause variations in amino acids. A non-synonymous SNP at the base of 183 bp changes threonine to arginine. Lec183 distinguished rodent tuber mutant plants better from their wild type in the amplification. The Lec183 marker detected a lectin gene SNP’s G allele and analyzed rodent tuber plants, which produced an amplification that determined the G allele level. The Lec183 is an effective marker for selecting rodent tuber-lectin mutation sites in large populations. This way helped obtain the rodent tubers with the highest anticancer bioactive compound more precisely and rapidly.

Keywords: Rodent tuber (Typhonium flagelliforme Lodd.), lectin gene, anticancer bioactive compound, specific primer design, SNAP, allele-specific marker

Key findings: Specific molecular markers designed with gene-based markers associated with anticancer bioactive compounds in rodent tuber (Typhonium flagelliforme Lodd.) plants are yet for study. Through primer lec183, the specific alleles in lectin genes can help differentiate the mutant and wild-type plants. The SNAP marker based on the lectin gene sequence could probably improve the accuracy of the selection of lectin anticancer compounds in rodent tuber mutant plants.