Several viruses currently found in the maize (Zea mays L.) fields showed the primary and most crucial one is the maize dwarf mosaic virus (MDMV) in Uzbekistan. The use of a combination of serological testing and next-generation molecular-genetic approaches helped assess the current reservoir of viruses observed in past disease outbreaks. The MDMV has gained proof to infect cereal grasses and maize. The presented results provide important strategies for the management of viral diseases in the sweet corn maize crop, as well as identifying potential future virus threats. The following study explores the distribution, symptoms, and diagnostics of MDMV. A pure homogeneous preparation of the virus immunized in the body of the experimental rabbit became the antiserum (Figure 1). In the isolated serum, determining the titer used the DID method, the AS titer was 1:16, and the amount of isolated AS was 12.5 ml. In addition, the conduct of molecular-genetic diagnosis used PCR. According to PCR results, MDMV appeared as the most common virus affecting maize crops in Uzbekistan. The PCR analysis revealed the maize sample with mosaic signs along the leaf veins and dwarfism was the causative agent of MDMV. The RT-PCR method performed relied on a fragment of the gene responsible for the synthesis of the MDMV shell.
Maize (Zea mays L.), cultivars, maize dwarf mosaic virus (MDMV), phytoviruses, plant diseases, yield-related traits, productivity
The diagnostic results showed a considerable spread of MDMV in the maize (Z. mays L.) fields of Uzbekistan. The study obtained and identified a specific MDMV serum by molecular-genetic methods.