The climatic conditions in Uzbekistan are highly favorable for cultivating leguminous crops, particularly the common bean (Phaseolus vulgaris L.). In the recent past, infections with the bean common mosaic virus (BCMV) have been evident in various crops across the country, leading to a decline in grain yield and quality. Phytoviruses infect bean plants and manifest through specific disease symptoms with considerable damage in leguminous crops, especially beans, mung beans, and peas. According to a monitoring conducted in various districts of the Tashkent Region, the PCR analysis revealed that BCMV was the most widespread virus. The collected BCMV-diseased plant samples underwent molecular-genetic identification based on the capsid protein (CP) gene. The PCR product succeeded in sequencing, with the resulting isolate being registered in the NCBI database under the name ‘Bean common mosaic virus isolate UZ-1’ with the accession number PQ442186.1. Phylogenetic analysis of this isolate revealed 97% similarity with the Chilean isolate (LI9539.1) and 99% similarity with isolates from phylogenetic lineages in Vietnam (LC775775.1), Nepal (MW620828.1), Russia (KF919300), and Africa (AF361337).
Common bean (P. vulgaris L.), RT-PCR, CP gene, bean common mosaic virus isolate ‘UZ-1,’ primer, phylogenetic analysis
Using RT-PCR, the bean common mosaic virus isolate ‘UZ-1’ attained identification in the common bean (P. vulgaris L.) plants. The nucleotide sequence of the CP gene, responsible for encoding the viral coat protein, sustained assessment. Bioinformatics analysis resulted in the phylogenetic tree for BCMV-‘UZ-1,’ which helped in determining the evolutionary origin of the virus.