Based on isolating Gluconacetobacter diazotrophicus from the intercellular spaces of aseptically produced sugar beet root (Beta vulgaris L.) seedlings in sucrose-containing culture conditions, the following study determined the interaction of G. diazotrophicus, a non-nodulating endophytic nitrogen-fixing bacterium. The entire root system gained intracellular colonization by G. diazotrophicus after inoculating the plant with the bacterium. Sugar beet seedlings’ root tips and cotyledons inoculated with GUS-labeled genes bore scrutiny under a light microscope to examine the blue-stained G. diazotrophicus in the root cells’ cytoplasm. The favorable environment within the cell helped produce the nitrogenase nif gene. Novel inoculations with G. diazotrophicus underwent investigation for their ability to promote the non-nodular endosymbiotic nitrogen fixation. The inoculations’ viability as a plant model for investigating the endosymbiotic theory of organelle generation in eukaryotic organisms is another vital question requiring answers.

Keywords: Sugar beet (B. vulgaris L.), GUS gene, G. diazotrophicus, nif nitrogen fixation, interaction, eukaryotic organisms

Key findings: Successful intervention of sugar beet (B. vulgaris L.) seedlings with GUS-labeled G. diazotrophicus occurred for the first time, expressing the possibility of fixing atmospheric nitrogen (N₂) as an alternative to Rhizobium bacteria.