B. AMANOV, K. MUMINOV, S. SAMANOV, F. ABDIEV, D. ARSLANOV, and N. TURSUNOVA
SUMMARY
The conducted study, with focus on the earliness and opening rate of bolls, and other economic traits of introgressive cotton lines, was carried out from 2017 to 2019 at the Institute of Genetics and Plant Experimental Biology, and Genetics and Evolution Biology Department, in Chirchik State Pedagogical Institute, Tashkent, Uzbekistan. Overall, the introgressive cotton lines were found to ripen two to 13 days earlier than a standard cotton cultivar C-6524. Particularly, in line L-РСМ, the vegetation period took 109 days and matured 12.3 days earlier than a standard cultivar check. According to the boll weight, the selected cotton lines were identified to have the best performance, as well as, good germination ability of seeds. The cotton line, Т-138, was found highly resistant to strains of Fusarium oxysporum f. sp. vasinfectum pathogenic fungi, while the lines, L-РСМ and L-138, had 80% to 100% resistance to Verticillium dahliaе fungi. The cotton lines, L-РСМ and L-141, were not infected with Fusarium solani fungi. The cluster analysis revealed that according to the economic traits, the first cluster included lines L-24 and L-138 and were found to be closely related to each other. The line, LРСМ, was included in the third cluster, and it was found that this cotton line was among the cultivars with the best performance for all the valuable economic traits. The comparative analysis of cotton lines resulted in a creation of a new medium-fiber cotton cultivar “Niso,” with the productivity of 3,830 to 5,650 kg ha-1, boll weight of 6.0 to 6.7 g, boll opening at 109.0 to 112.0 days, type IV fiber, 1000seed weight of 118.0 to 120.0 g, fiber percentage ranging from 36.9% to 41.4%, fiber length at 34.0 to 35.9 mm, and relative tensile strength (34.6 cN/tex). Cotton lines obtained on the basis of intergenomic introgression methods were included in the leading “Cotton gene pool” (Reference of the Academy of Sciences of the Republic of Uzbekistan №4/1255-2473 dated November 12, 2020). These introgressive cotton lines made it possible to enrich the collection of cotton, to evaluate wild cotton species, and to form a system of information and analysis of the electronic database of adaptable stress factors and genetically enriched samples.
Citation: Fernandez ECJ, Nuñez JPP, Gardoce RR, Manohar ANC, Bajaro RM, Lantican DV (2023). Genetic purity and diversity assessment of parental corn inbred lines using ssr markers for Philippine hybrid breeding. SABRAO J. Breed. Genet. 55(3): 598-608. http://doi.org/10.54910/sabrao2023.55.3.1.
Summary
Accurate data and relevant insights on parental corn inbred lines’ and hybrids’ genetic purity and diversity are essential for hybrid development and seed production. Here, the genetic purity and diversity of 19 parental yellow corn inbred lines were assessed using SSR markers. A total of 91 SSR markers were utilized, of which 61 were highly polymorphic and had high polymorphism information content value (PIC = 0.379). Genetic purity and diversity parameters were calculated from the generated SSR marker data. Observed pairwise genetic distances ranging from 0.257 to 0.808 implied high genetic dissimilarities among the accessions. Cluster analysis separated the parental lines into three distinct subclusters, which can potentially be a basis for generating heterotic groupings among the parental lines. Eight (8) out of the 19 parental lines showed considerable residual heterozygosity of ≤10%. Inbred line CML 431 displayed complete homozygosity across all 61 SSR markers. Inbred lines that have residual heterozygosity of >15% need purification through further breeding and selection. Out of the resultant F1 hybrids analyzed, only four (4) showed genetic impurity of ≤10%. It may be attributed to the intrinsic genetic impurity of parental line CML 452 (21.67%). In contrast, hybrids generated from two genetically pure parents (e.g., CML 431 and CML 575) showed low to no off-types. Overall, genetic purity and diversity determination of promising parental lines can be valuable for future yellow corn breeding programs in the Philippines.
corn, SSR markers, genetic purity, genetic diversity, hybrid development
The genotyping strategy employed in the study proved cost-efficient and effective in
terms of identifying whether the corn genotypes are genetically pure or contaminated. Corn breeders
can utilize the generated clusters as a basis for designing cross-combinations. Finally, the achieved set
of SSR markers determined can help further assess successful hybridization among the corn
genotypes used in this study.
Two field experiments were carried out on a private farm at Wadi El-Natrun (latitude of 30.48° N and longitude of 30.50° E), Beheira Governorate, Egypt, in the 2019–2020 and 2020–2021 seasons, to find out the effect of adding zeolite as a soil conditioner and potassium fertilizer on growth, yield, and quality of sugar beet crop (Beta vulgaris L. var. saccharifera) grown in sandy soil conditions. The present work included 12 treatments, which were the combinations of four zeolite levels (Zero, 476, 952, and 1,428 kg ha-1), and three levels of potassium in the form of potassium sulfate (119, 178.5, and 238 kg K2.SO4 ha-1), which were added as a soil application. The treatments were arranged in a complete block design in a split plot with four replications. The results showed that higher values of the photosynthetic pigments, root diameter, fresh and foliage weights plant-1, as well as, sucrose and extracted sugar percentages, quality index, yields of the root, top, and sugar ha-1, were obtained by adding 1,428 kg zeolite, compared with the other levels of zeolite, in both seasons. However, sodium, alpha-amino N contents in the root, and sugar lost to molasses% were insignificantly affected by zeolite rates in both seasons. Application of 238 kg K2.SO4 ha-1significantly resulted in the highest values of photosynthetic pigments, root dimensions, sucrose%, and root potassium content. In addition, extracted sugar %, quality index, root, top, and sugar yields ha-1 were increased compared with the other lower K-sulphate levels in both seasons. On the contrary, sugar lost to molasses% was insignificantly affected by applied potassium sulfate in both seasons. The maximum values of root diameter, fresh weight, yields of root and top ha-1 in both seasons, and also sugar yield in the second season were produced from the interaction between applying 1,428 kg zeolite and 238 kg potassium sulfate ha1.
N.A.R. MERZA, H.A. ATAB, Z.H. AL-FATLAWI, and S.K.A. ALSHARIFI
Citation: Merza NAR, Atab HA, Al-Fatlawi ZH, Alsharifi SKA (2023). Effect of irrigation systems on rice productivity. SABRAO J. Breed. Genet. 55(2): 587-597. http://doi.org/10.54910/sabrao2023.55.2.30.
Summary
In light of the scarcity of irrigation water, extreme wastage of water, saline soils, and the dominance of traditional water management methods, the presented research transpired in 2020 in the Directorate of Agriculture, ALHashimiya area, Hilla City, Iraq. The latest study aimed to investigate the effects of subsurface drip irrigation system (SDIS) and flood irrigation system (FIS), with three drip irrigation distances of 18, 20, and 22 cm on two rice (Oryza sativa L.) cultivars, i.e., ‘Tarm Hashemi’ (TH) and ‘Daillman Mazandarani’ (DM). The irrigation systems (SDIS and FIS) significantly impacted the growth and productivity traits of the rice crop. The SDIS ensures the addition of an appropriate amount of water to the plants by keeping wet the root zone without wasting water compared with the flood irrigation system. The interaction of rice cultivar Tarm Hashemi, SDIS, and drip irrigation distance (DID of 18 cm) resulted in the best performance for root growth traits, i.e., root length and root fresh and dry weight (17.66 cm and 1.80 and 0.85 g, respectively). It also provided the highest plant vigor index (PVI), biological, and grain yield (71.15 cm, 1,747.23, and 608.26 g.m-2, respectively). Cultivar Tarm Hashemi proved superior to cultivar Daillman Mazandarani in managing the morpho-yield traits. The subsurface drip irrigation system during the growing season helped increase the productivity of the rice crop compared with the traditional irrigation system. Compared with FIS, the SDIS not only improved and enhanced the growth and grain yield, but also saved abundant water and nutrients.
Rice (Oryza sativa L.), cultivars Tarm Hashemi (TH) and Daillman Mazandarani (DM), subsurface drip irrigation system (SDIS), flood irrigation system (FIS), drip irrigation distance (DID)
The study focused on achieving the best growth results for the rice (Oryza sativa L.) crop, with the highest productivity emerging with the subsurface drip irrigation system and the planting distance of 18 cm. The reaction of rice cultivar Tarm Hashemi (TH) to the subsurface drip irrigation system gave the highest studied characteristics compared with the rice cultivar Daillman Mazandarani (DM).