M.H. KALERI, W.A. JATOI, M. BALOCH, S.N. MARI, S. MEMON, S. KHANZADA, L. RAJPUT, and K. LAL
Citation: Kaleri MH, Jatoi WA, Baloch M, Mari SN, Memon S, Khanzada S, Rajput L, Lal K (2023). Heterotic effects in sunflower hybrids for earliness and yield traits under well-watered and stressed conditions. SABRAO J. Breed. Genet. 55(3): 609-622. http://doi.org/10.54910/sabrao2023.55.3.2.
Summary
The climate is continuously changing, consequently increasing the drought-affected areas. As such, it challenges breeders to develop adaptive and drought-tolerant sunflower (Helianthus annuus L.) cultivars through evaluation and inducing genes tolerant to drought. Hence, the recent study aimed to assess the sunflower hybrids during 2019–2020 under well-watered and stressed conditions in a splitplot design with four replications at the Sindh Agriculture University, Tandojam, Pakistan. The observed data determined heterotic effects among 15 F1 hybrids for days to 75% flowering, days to 75% maturity, stem diameter, head diameter, biological yield plant-1, seeds head-1, seed index, and seed yield plant-1. The mean squares due to genotypes, treatments, and genotype by treatment were significant for all the traits, which exhibited that genotypes performed significantly across the environments for the above-cited traits. The F1 hybrids, such as, Mehran × Pehawar-93, gave maximum negative heterotic effects for phonological traits which will benefit the development of short-duration sunflower hybrids. Further, F1 hybrids like Thatta × UC-666 displayed higher heterotic effects for head diameter, stem diameter, number of seeds plant-1, seed index, and biological plant-1 and PSF-025 × B2 and HO.1 × B2 gave higher heterotic effects for the number of seeds head-1 and seed yield plant-1 under stress environment.
D.M. TARIGAN, W.A. BARUS, A. MUNAR, and A. LESTAMI
Citation: Tarigan DM, Barus WA, Munar A, Lestami A (2023). Exploration and morphological characterization of phosphate-solubilizing and nitrogen-fixing bacteria in saline soil. SABRAO J. Breed. Genet. 55(2): 550-563. http://doi.org/10.54910/sabrao2023.55.2.27.
Summary
Phosphate-solubilizing and nitrogen-fixing bacteria are crucial in increasing soil fertility and restoring soil properties damaged by salinity and other abiotic environmental factors. The presented study aims to explore and identify the morphological characteristics of phosphate-solubilizing and nitrogen-fixing bacteria in saline soil. This study took place from August until November 2022 in the field and laboratory of the Faculty of Agriculture, Universitas Muhammadiyah Sumatera Utara, Medan, Indonesia. Isolation of potential microbes proceeded to characterize phosphate-solubilizing and nitrogen-fixing bacteria. Sampling began with the soil planted with rice and palm oil at the sampling location. Morphological parameters observed were color, form, margin, surface, and elevation of pure colonies. The result showed that exploring various species in saline soil revealed 19 colonies and 14 cells of phosphate-solubilizing bacteria and 16 colonies and 14 cells of nitrogen-fixing bacteria. The pure colonies of the phosphate-solubilizing and nitrogen-fixing bacteria showed differences in the morphological characteristics, i.e., color, form, margin, surface, and elevation. The potential microbes obtained sought to increase soil fertility and crop production.
Exploration and identification of morphological characteristics is a must action to analyze potential microbes in depth to improve the quality of saline soils to increase crop productivity.
AAMER AL-MATHIDY, ZERAVAN A.S. AL-DOSKEY, and MUNA O.M. SHEHAB
Citation: Al-Mathidy A, Al-Doskey ZAS, Shehab MOM (2023). Numerical taxonomy of the genus Rosa L. (Rosaceae) grown in the Kurdistan Region of Iraq. SABRAO J. Breed. Genet. 55(2): 442-452. http://doi.org/10.54910/sabrao2023.55.2.16.
Summary
The presented research aimed to study the numerical taxonomy of the genus Rosa L. and to identify and differentiate its various species grown in different regions in Kurdistan, Iraq. This study proceeded in the 2021–2022 season at the College of Education of Pure Science, University of Mosul, Mosul, Iraq. Forty morphological quantitative and qualitative characteristics, including vegetative and reproductive traits (leaves, flowers, fruits, seeds, and pollen grains), gained taxonomic analysis. From there, selection of 12 morphological features finally drew polygonal shapes for the concerned species. The analysis of the polygonal shapes revealed the species owned significant variations in these forms. The similarity among these species ranged between 0.5% to 91.0%, and the highest level of similarity (91.0%) occurred between the two species Rosa canina var. ‘canina,’ and Rosa canina var. ‘verticillacantha,’ and the lowest (0.05%) emerged from the species. R. elyamaitica and R. dumalis subsp. boissieri. From the cluster analysis, the UPGMA dendrogram separated the 13 species into three main groups and subgroups. The first main groups, divided into two subgroups, included the species R. canina var. ‘canina,’ R. canina var. ‘verticillacantha,’ R. canina var. ‘dumetorum,’ R. canina var. ‘deseglisei,’ and R. dumas subsp. boissieri. The second main groups included the species R. eiyamaitica and R. heckeliana subsp. orientalis. The second subgroup included the species R. gallica, R. centifolia, and R. damascena. Moreover, the third main group included the species R. foetida, R. foetida var. bicolor, and R. hemisphareaca.
Rosa L. (Rosaceae), numerical taxonomy, species, genetic variations, quantitative and qualitative traits, cultivars, UPGMA, cluster analysis
Using numerical taxonomy helped identify and differentiate the 13 taxa of the genus Rosa L. (Rosaceae), grown in the Kurdistan region of Iraq, based on quantitative and qualitative parameters, comprising vegetative morphological and reproductive traits.
Citation: Al-Haidari HAM, Al-Tamimi AJT (2023). Physical and chemical mutagenesis in catharanthus roseus plant using SCoT markers. SABRAO J. Breed. Genet. 55(2): 399-406. http://doi.org/10.54910/sabrao2023.55.2.12.
Summary
The current study comprising molecular identification of the mutagenic effects of gamma rays and sodium azide on Catharanthus roseus using scot markers took place at the Department of Biology, University of Kufa, Iraq. The seeds of two cultivars, i.e., ‘victory carmine’ and a local variety (pink variety) of Catharanthus roseus L (carmine var.) received irradiation with two doses of gamma rays (50 Gray and 75 Gray). Afterward, these got soaked in sodium azide with four concentrations (0.2%, 0.4%, 0.6%, and 0.8%) to induce the genetic variation in the cultivar. The non-treated seeds served as control treatments for comparison with treated ones. Accomplishing irradiation was at an average of 18 Gy/h using Cobalt-60. The effects of gamma rays and sodium azide treatments’ evaluation were through 10 start codon targeted (SCoT) DNA markers. The higher number of distinctive fingerprints was three produced by primers SCoT-26, while primer SCoT-60 provided a distinct fingerprint for only one treatment. However, primer SCoT-40 failed to recognize any treatment with a distinctive fingerprint. The highest molecular size (2488 bp) came from primer SCoT-54, whereas the lower molecular size (143 bp) resulted in primer SCoT-12. Primer SCoT-33 provided the highest value for chief and unique bands, reaching 33 and 18, respectively. The highest number of amplified bands was 77 bands in primer SCoT-54. Primer SCoT-60 produced the highest value for monomorphic bands, primer SCoT-12 gave the highest value for polymorphic bands, polymorphism, and discriminatory (15, 68.18, and 20.83, respectively), and primer SCoT-9 produced the highest value for efficiency.
In the presented molecular study, using ScoT markers were capable of assessing the genetic variations generated by gamma rays and sodium azide treatments with diverse doses and concentrations, respectively.
Citation: Khanishova MA, Azizov IV (2023). Evaluation of interspecific wheat hybrids (T. Durum × T. Aestivum) for spike-related traits. SABRAO J. Breed. Genet. 55(2): 291-297. http://doi.org/10.54910/sabrao2023.55.2.2.
Summary
The presented study analyzed the inheritance via heterosis of interspecific F1 hybrids between durum and bread wheat over better parents and ratios of dominance for spike-related traits affecting their productivity and transmission to future segregating populations. The research transpired in the crop seasons of 2017–-2019 at the Institute of Molecular Biology and Biotechnologies, Azerbaijan National Academy of Sciences, Baku, Azerbaijan. The hybridization program used eight durum wheat (Triticum durum Desf) genotypes, i.e., Shark, Karabagh, Barakatli-95, Tartar, Garagilciıg-2, Vugar, Mirbashir- 50, and Shiraslan-23, and 13 bread wheat (T. aestivum L.) cultivars, viz., Mirbashir-128, Kyrmyzygul-1, Gobustan, Layaqatli, Kyzyl Bughda, Gunashli, Taleh-38, Sheki-1, Murov, Nurlu-99, Aran, and two foreign cultivars Bezostaya-1 (Russia) and Dagdash (Turkey) to generate 24 interspecific F1 hybrids. Studying the heterosis over better parents and degree of dominance in these interspecific F1 hybrids compared their performance with parental genotypes for spike-related traits, i.e., spike length, spikelets per spike, grains per spike, and grain weight per spike. In the F1 interspecific hybrids’ evaluation, the recorded desirable positive heterosis over better parents and dominance ratios appeared in cross combinations, i.e., Barakatli-95 × Gobustan, Karabagh × Mirbashir-128, Karabagh × Shark, Sheki-1 × Gobustan, Murov × Daghdash, Nurlu-99 × Layaqatli, Gobustan × Sheki-1, and Mirbashir-50 × Shiraslan-23 for spike-related traits. However, the F1 hybrid Garabagh × Gobustan revealed negative heterosis and depression for yield-related traits.
Interspecific wheat hybrids (T. durum × T. aestivum), parental genotypes, heterosis, dominance, spike-related traits, productivity
The study identified the promising durum by bread wheat F1 hybrids in terms of heterotic and dominance effects over better parents for yield indicators that can benefit future breeding work to obtain high-yielding wheat genotypes.
M.M. JAVAID, N. AHMAD, A. JAVED, M. MAKHDOOM M. SALEEM, M. OWAIS, M. NADEEM, S. RAHMAN, S. MEHBOOB, S. NAZ, A. REHMAN, J. AHMED, and M.H. TANVEER
SUMMARY
The wheat blast caused by the Magnaporthe oryzae Triticum pathotype has become a serious threat to wheat production in Pakistan and India after its severe outbreak in Bangladesh in 2016 and intermittent occurrence since then. Thus, planned systemic surveillance of wheat crops in two phases in 2018–2019 and 2019–2020 began to determine the status of wheat blasts in Punjab, Pakistan. In the first phase, a survey of 160 wheat fields in districts in Punjab ensued for typical blast symptoms. The surveillance‟s second phase occurred after the threshing of the wheat crop, with composite grain samples collected from all over Punjab, then analyzed in the lab. The surveillance results indicated that all the wheat fields were free from the typical blast symptoms. Similarly, none of the analyzed samples showed the presence of wheat blast pathogens. Therefore, the study validates that this dreadful disease (blast) does not exist in Punjab, Pakistan. Hence, the reason to remain calm needs dissemination among the farming community and the training of technical agriculture extension staff for vigilance in the future for monitoring this disease in different agro-ecological zones of Punjab.
Key Findings: Thorough surveillance of fields during the years 2018–2019 and 2019–2020 wheat crop seasons, as well as seed analysis from all over Punjab, depicted no infestation of wheat blast in Punjab, Pakistan.
Papan P, Chueakhunthod W, Poolsawat O, Arsakit K, Tharapreuksapong A, Tantasawat PA
SUMMARY
Cercospora leaf spot (CLS) resistance is a highly desirable trait for mungbean (Vigna radiata [L.] Wilczek) production in Thailand. ‘V4718’ is a vital resistance source that shows high and stable resistance to CLS disease. A previous study identified a major quantitative trait locus (QTL) (qCLSC72V18-1) controlling CLS resistance and found the marker (I16274) that was located closest to the resistance gene by using F2:9 and F2:10 recombinant inbred line populations derived through a cross between ‘V4718’ and the susceptible variety ‘Chai Nat 72’ (‘CN72’). Here, we evaluated three newly reported simple sequence repeat (SSR) markers and one InDel marker together with six previously identified markers that were linked to qCLSC72V18-1 to further identify the markers that were located close to this QTL. By performing bulk segregant analysis on two validation populations, we found that two SSR markers (Vr6gCLS037 and Vr6gCLS133) and one InDel marker (VrTAF5_indel) were putatively associated with CLS resistance. Of these markers, only the VrTAF5_indel marker showed a significant association with the CLS resistance gene with a logarithm of odds score > 3 across the phenotypic data for 2016 and 2018. QTL analysis with inclusive composite interval mapping revealed that the VrTAF5_indel marker was integrated into the genetic map with other previously identified markers. The I16274 and VrTAF5_indel markers flanking the QTL of interest accounted for 41.56%-60.38% of the phenotypic variation with genetic distances of 4.0 and 5.0 cM from the resistance gene, respectively. Both markers together permitted only 0.40% recombination with the CLS resistance gene in markerassisted selection and thus could be useful in future breeding efforts for CLS resistance in mungbean.
Daryanto A, Syukur M, Sobir, Maharijaya A, Hidayat P
SUMMARY
The cotton aphid (Aphis gossypi), is one of the most devastating insect pests for chili pepper that damages the crop and transmits several viruses. Thus far, there is no commercial chili cultivar with effective resistance to cotton aphids (CA). The present study aimed to develop a reliable and practical screening protocol in chili peppers for aphids resistance in the tropical areas. Three no-choice test methods i.e., seedling cage, detached leaf, and clip cage tests have been developed. The experiment was conducted in a randomized complete block design (RCBD) with four replications in a greenhouse at the Gunung Putri, Bogor, West Java, Indonesia. Seven chili pepper genotypes belong to the species Capsicum annuum L. provided by the Department of Agronomy and Horticulture, IPB University, Bogor, Indonesia were used in this study. Chili pepper resistance to aphids was observed through the number of aphids progeny, and aphids fecundity among the genotypes. Two chili genotypes consistently showed low infestation while other genotypes showed the highest infestation with three screening methods. All the methods have a high heritability value (90%–91%), indicating that the evaluated chili genotypes could be used to determine effective screening methods. The correlation between the detached leaf and clip cage tests was significant (r = 0.84, P ≤ 0.05). Therefore, the clip cage test could be used as a reliable and practical screening test for the assay of chili peppers resistance to CA infestation. These information will be helpful in the development of aphid resistant cultivars in the future.
Cinchona species were widely used as ancient medicines for different diseases because they contain the active component quinine and its derivatives. However, studies on the molecular aspects of cinchona, including its genetic diversity, have not been reported because most previous works focused on the administration of the antimalarial cinchona alkaloid. Quinine is also being tested as alternative compound for the treatment of Covid-19. The Junghuhn Natural Reserve in Indonesia contains three different types of cinchona plants, namely, Cinchona calisaya, Cinchona pubescens, and Cinchona sp. Given that the genetic diversity and kinship of these species have never been studied, collecting data on the cinchona gene pool has become imperative. This study analyzed the genetic diversity of the cinchona species in the Junghuhn Natural Reserve, Indonesia, by using eight RAPD markers, i.e., OPA-2, OPA-9, OPB-02, OPB-03, OPB-04, OPB-05, OPB-7, and OPJ-07, during 2020 at the University of Jenderal Soedirman, Purwokerto Indonesia. Polymorphic band data were obtained. Then, phenogram analysis was conducted by using UPGMA and maximum parsimony with MEGA7. The RAPD profiles of Cinchona species (C. calisaya, C. pubescent, and Cinchona sp.) revealed polymorphism with different markers, i.e., OPA-2 (90%), OPB-2 (75%), OPB-5 (75%), OPB-3 (66.66%), OPB-4 (66.66%), OPB-7 (66.66%), OPJ-7 (66.66%), and OPA-9 (58.33%) sequentially with total polymorphism (70.62%). C. calisaya was identified as the most distinctive species. UPGMA yielded a coefficient of 0.200 and two distinctive groups: Group I, which comprised C. pubescens and Cinchona sp. with the pdistance value of 0.333, and Group II, which contained C. calisaya. Ixora sp. was treated as an outgroup plant. The topology of the dendrogram was consistent with that of the UPGMA dendrogram. Results may be used for the further exploration of the genetic diversity of cinchona species.